Respiratory Functions of Mitochondria Isolated from Stress-Susceptible and Stress-Reistant Pigs

Ga, Brooks; Rg, Cassens

doi:10.2527/jas1973.373688x

Cited by 24 publications

(8 citation statements)

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“…But Cheah (1973) found no differences in the respiratory function of muscle mitochondria isolated from Pietrain and Land-.race pigs when measured over a wide range of pH values. Brooks and Cassens (1973) measured the performance of muscle mitochondria of stress-susceptible and stress-resistant pigs at different temperatures and found no significant differences between the two types of pigs in either state 3 respiration rate or RCI values. also found no differences in respiratory rate or RCI between muscle mitochrondria isolated from halothane-sensitive and normal pigs.…”

Section: Mitochondriamentioning

confidence: 98%

“…Extrapolating to the in vivo situation suggests that the initial increase in heat production does not arise primarily by activation of mitochrondrial ATPase by halothane. The increased 02 consumption observed by Berman et al (1970) may have arisen secondarily to an increase in muscle temperature (Brooks and Cassens, 1973) as well as to an increase in sarcoplasmic calcium (Ca 2+) levels.…”

Section: Mitochondriamentioning

confidence: 99%

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A Review of the Role of Swine Skeletal Muscle in Malignant Hyperthermia

Campion

Topel

1975

Journal of Animal Science

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The syndrome of malignant hyperthermia (MHS) has been visualized from the point at which sarcoplasmic Ca 2+ concentration becomes sufficient to stimulate phosphorylase kinase and, indirectly, myosin ATPase. Elevated lv 2+ e els of cellular Ca may cause a functional uncoupling of oxidative phosphorylation as the mitochondria utilize high energy intermediates to sequester Ca 2+. Consequently, ATP is consumed, and the cellular levels of ADP and inorganic phosphorus increases, further stimulating glycolysis as the MHS develops. As the mitochondria sequester Ca 2+, 02 consumption is increased and this, in addition to the stimulating effect of temperature, may explain the observed increase in O2 consumption.However, the triggering mechanisms that allow the various body functions, when exposed to halothane, to combine in an abnormal manner to produce what we recognize as malignant hyperthermia remain obscure. (

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Section: Mitochondriamentioning

confidence: 98%

Section: Mitochondriamentioning

confidence: 99%

A Review of the Role of Swine Skeletal Muscle in Malignant Hyperthermia

Campion

Topel

1975

Journal of Animal Science

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“…In all these studies, MRA was analysed with permeabilised muscle fibres. Considering studies that investigated the respiratory activities of isolated mitochondria, no differences of Pyr/Mal-or glutamate/malate (Glu/Mal)-MRA values in pig muscle samples with different pH values could be found pH effect on structure and mitochondria in pork (Brooks and Cassens, 1973;Campion et al, 1975;Rasmussen et al, 1996). However, Eikelenboom and van den Bergh (1973) found lower Glu/Mal-MRA and Cheah (1973) found lower Succ-MRA results in isolated muscle mitochondria from stress-susceptible (Pietrain) compared with -resistant pigs (Dutch Landrace, Large White).…”

mentioning

confidence: 99%

The relationship of pork longissimus muscle pH to mitochondrial respiratory activities, meat quality and muscle structure

Popp

Wicke

Klein

et al. 2015

Animal

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The way the pH falls post-slaughter has an impact on meat quality. Pork longissimus muscles (n = 48) were sorted in fast-(FG) (n = 15) and normal glycolysing (NG) (n = 33) muscles according to the post-slaughter pH 45 min values (FG < 6.0; NG > 6.0). FG muscles (5.84 ± 0.04) compared with NG muscles (6.27 ± 0.04) were accompanied with higher temperature, electrical conductivity, lightness and yellowness, and reduced grill loss and shear force values ( P < 0.05), but there were no pH-dependent changes of the drip loss and redness results. FG muscles had higher ( P < 0.05) percentages of fast-twitch glycolytic and lower proportions of fast-twitch oxidative and slow-twitch oxidative ( P < 0.05) muscle fibres. The study confirms the relationship of pH value to meat quality and muscle fibre characteristics also showing that pH values have no impact on intrinsic mitochondrial respiratory function.

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“…On the other hand, Nelson et aL (1972) have described normal calcium regulation by the sarcoplasmic reticulum in malignant hyperthermia-prone pigs. Also, Brooks and Cassens (1973) and Cheah (1973) did not observe any mitochondrial defect as a primary factor in the porcine stress syndrome reported by Topel et al, 1968. The purpose of this investigation was to study the ATPase activity of two skeletal muscle organelles, mitochondria and sarcoplasmic reticulum, isolated from pigs of similar breeding that were identified as stress susceptible (SS) or stress resistant (SR) at weaning age, depending on their response to halothane anesthesia. In addition, mitochondrial respiratory activity was measured and evaluated.…”

mentioning

confidence: 59%

In Vitro Studies of Mitochondrial and Sarcoplasmic Reticulum Function in Muscle of Stress Susceptible Pigs

Campion

Topel

Christian

1976

Journal of Animal Science

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Seven stress susceptible (SS) and six stress resistant (SR) pigs were identified by response to halothane anesthesia. Mitochondria and heavy sarcoplasmic reticulum fragments were isolated from biopsies of the semimembranosus muscle. The muscle pH was significantly lower (P<.01) in the SS pigs compared to the SR pigs at biopsy. The mitochondrial respiratory traits, state 3 and state 4 respiration, respiratory control index and ADP/0 ratio, however, were not significantly different (P>.05) for the two groups of pigs. Mitochondrial ATPase activity and sarcoplasmic reticulum Mg-ATPase activity were not significantly different (P>.05) for the muscles of SS and SR pigs. The MgCa-ATPase activity of sarcoplasmic reticulum isolated from the muscle of SS pigs was significantly higher (P<.01) than that observed for SR pigs. No differences (P>.05) in mean values for pH, percentage reflectance and percentage transmission values of the longissimus muscle were observed between the SS and SR pigs, but greater variation in values for these traits was observed in muscle from SS pigs. (

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Respiratory Functions of Mitochondria Isolated from Stress-Susceptible and Stress-Reistant Pigs

Cited by 24 publications

References 0 publications

A Review of the Role of Swine Skeletal Muscle in Malignant Hyperthermia

A Review of the Role of Swine Skeletal Muscle in Malignant Hyperthermia

The relationship of pork longissimus muscle pH to mitochondrial respiratory activities, meat quality and muscle structure

In Vitro Studies of Mitochondrial and Sarcoplasmic Reticulum Function in Muscle of Stress Susceptible Pigs

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