Respiratory effects of cyclic AMP following injection into the nucleus tractus solitarius of rats

Barraco, Robin A.; Schoener, Eugene P.; Polasek, Petra M.; Simpson, Lori; Janusz, C. J.; Parizon, M.

doi:10.1016/0028-3908(88)90032-9

Cited by 16 publications

(1 citation statement)

References 13 publications

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“…In this particular study, in order to identify the involvement of the intracellular signal transduction pathway in the induction of REM sleep, we chose a specific inhibitor, SQ22536, to block AC-dependent cAMP (Fabbri et al, 1991;Shi and Bunney, 1992;Johnson et al, 1997;Natsume and Kometani, 1999;Froehlich and Wand, 1997;Marks and Birabil, 2000). The local microinjection of SQ22536 and other signal transduction-altering compounds into the brain of freely moving animals has already been shown to be an important technique when studying the involvement of signal transduction pathways modulating behaviors (Kantak et al, 1981;Barraco et al, 1988;De Lima and Davis, 1995;Cook et al, 1995;Capece and Lydic, 1997;Marks and Birabil, 2000). However, a major limitation of the microinjection method relates to the diversity in the neurochemical nature of the neuronal population affected by the drug application.…”

Section: Discussionmentioning

confidence: 99%

Novel Role of Brain Stem Pedunculopontine Tegmental Adenylyl Cyclase in the Regulation of Spontaneous REM Sleep in the Freely Moving Rat

Datta¹,

Prutzman²

2005

Journal of Neurophysiology

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Physiological activation of kainate receptors and GABA(B) receptors within the pedunculopontine tegmentum (PPT) is involved in regulation of rapid-eye-movement (REM) sleep. Because these two types of receptors may also directly and/or indirectly activate the intracellular cyclic adenosine monophosphate (cAMP) signaling pathway, we hypothesized that this signaling pathway may be involved in the PPT to regulate spontaneous REM sleep. To test this hypothesis, four different doses (0.25, 0.50, 0.75, and 1.0 nmol) of a specific adenylyl cyclase (AC) inhibitor, 9-(tetrahydro-2-furanyl)-9H-purin-6-amine (SQ22536), were microinjected bilaterally (100 nl/site) into the PPT, and the effects on REM sleep in freely moving chronically instrumented rats were quantified. By comparing alterations in the patterns of REM sleep after control injections of vehicle or one of the four different doses of SQ22536, the contributions made by each dose of SQ22536 to REM sleep were evaluated. The results demonstrated that the local microinjection of AC inhibitor SQ22536 into the PPT decreased the total amount of REM sleep for 3 h and increased slow-wave sleep (SWS) for 2 h in a dose-dependent manner. This reduction in REM sleep was due to increased latency and decreased frequency of REM sleep episodes. These results provide evidence that inhibition of AC within the PPT can successfully reduce REM sleep. These findings suggest that activation of the cAMP-signaling pathway within the cholinergic cell compartment of the PPT is an intracellular biochemical/molecular step for generating REM sleep in the freely moving rat.

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Section: Discussionmentioning

confidence: 99%

Novel Role of Brain Stem Pedunculopontine Tegmental Adenylyl Cyclase in the Regulation of Spontaneous REM Sleep in the Freely Moving Rat

Datta¹,

Prutzman²

2005

Journal of Neurophysiology

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Purinergic receptors in the nucleus tractus solitarius mediate distinct cardiorespiratory response patterns

Barraco¹,

Ergene²,

El-Ridi³

1993

Drug Development Research

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The purpose of the study was to examine the role of brainstem purinergic receptors in mechanisms of cardiorespiratory control mediated by the nucleus tractus solitarius (NTS), the major integrative site in the brainstem involved in the regulation of cardiorespiratory function and the reflex coordination of cardiorespiratory response patterns. Microinjections of selective agonists for purinergic receptor subtypes were made into the caudal NTS of anesthetized, spontaneous breathing rats. CGS 21680, a selective agonist for adenosine A , , receptors, elicited pronounced, dose-related decreases in blood pressure, whereas cyclopentyladenosine (CPA), a selective agonist at adenosine A, receptors, elicited dose-related increases in blood pressure. These depressor and pressor response patterns were completely and selectively blocked, respectively, by pretreatment with CGS 15943A, a selective A,, receptor antagonist, and by 1,3-dipropyl-8-cyclopentylxanthine (DPCPX) a selective A, receptor antagonist. 5'-N-ethylcarboxamidoadenosine (NECA), an analog exhibiting mixed agonist actions at adenosine receptor subtypes, elicited both depressor and pressor responses depending on the dosage. Additionally, in contrast to the findings with CGS 21680 and CPA, only NECA elicited very pronounced, dose-related decreases in respiratory rate at dosages which did not affect blood pressure. Related lines of evidence suggested that NECAinduced depression of respiration was mediated via receptors. Microinjections of a,P-MeATP, a selective agonist for P , , receptors, elicited apnea, and these effects were blocked by suramin, a selective antagonist at P , , receptors, but not by DMPX, a methylxanthine exhibiting mixed antagonist properties at P, adenosine receptors. Moreover, similar microinjections of 2-MeSATP, a selective agonist at P , , receptors, were ineffective in eliciting cardiorespiratory responses even at 50-fold higher dosages. These results indicate that selective in vivo activation of purinergic receptors in the NTS may elicit distinct cardiorespiratory response patterns and it i s likely that brainstem purinergic receptors may therefore play a crucial role under both physiologic and pathophysiologic conditions. o 1993 WiIey-Liss, Inc.

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P1 Receptors in the Respiratory System

Biaggioni

Feoktistov

2001

Purinergic and Pyrimidinergic Signalling II

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Respiratory effects of cyclic AMP following injection into the nucleus tractus solitarius of rats

Cited by 16 publications

References 13 publications

Novel Role of Brain Stem Pedunculopontine Tegmental Adenylyl Cyclase in the Regulation of Spontaneous REM Sleep in the Freely Moving Rat

Novel Role of Brain Stem Pedunculopontine Tegmental Adenylyl Cyclase in the Regulation of Spontaneous REM Sleep in the Freely Moving Rat

Purinergic receptors in the nucleus tractus solitarius mediate distinct cardiorespiratory response patterns

P1 Receptors in the Respiratory System

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