Respiratory-deficient mutants of Torulopsis glabrata, a yeast with circular mitochondrial deoxyribonucleic acid of 6 mu m

O'Connor, R M; McArthur, C. R.; Clark-Walker, G. D.

doi:10.1128/jb.126.2.959-968.1976

Cited by 47 publications

(15 citation statements)

References 22 publications

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“…The contour length of S. cerevisiae mtDNA ranges from 21 to 25 um (51,96), corresponding closely to the molecular mass determination of 46 x 106 daltons derived from sedimentation data (15). The mtDNA of several other yeasts has been reported (89,90) to range in contour length from one-quarter to one-half that of S. cerevisiae.…”

Section: Nucleic Acid Studies In Yeastsupporting

confidence: 60%

Genome comparison in yeast systematics: delimitation of species within the genera Schwanniomyces, Saccharomyces, Debaryomyces, and Pichia.

Price¹,

Fuson²,

Phaff³

1978

Microbiological Reviews

168

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Section: Nucleic Acid Studies In Yeastsupporting

confidence: 60%

Genome comparison in yeast systematics: delimitation of species within the genera Schwanniomyces, Saccharomyces, Debaryomyces, and Pichia.

Price¹,

Fuson²,

Phaff³

1978

Microbiological Reviews

168

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“…In addition, conflicting results have led to the lack of agreement concerning the effects of EB on nucleic acids in living organisms (McMurray and van Holde, 1991). One of the best known applications of EB is the irreversible destruction of mitochondrial genomes in some yeasts and fungi (Slonimski et al, 1968;Goldring et al, 1970;Whittaker et al, 1972;O'Connor et al, 1976;Niagro and Mishra, 1989). Although the molecular mechanism underlying this phenomenon remains unclear, it has been established that EB inhbits de novo synthesis of mitochondrial DNA (mtDNA) as well as stimulating a rapid destruction of pre-existing mtDNA molecules, resulting in a 100% conversion of a wild-type culture into a respiratory-deficient one.…”

Section: Introductionmentioning

confidence: 99%

The in vivo effects of ethidium bromide on mitochondrial and ribosomal DNA in Candida parapsilosis

Maleszka

1994

Yeast

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The ability of Candida parapsilosis to grow in the presence of high levels of ethidium bromide (EB) has been explored to study the effects of this intercalating dye on DNA in vivo. By employing confocal microscopy we have determined that EB penetrates the cellular membranes and binds rapidly to the nucleolus, whereas mitochondrial DNA becomes stained after a longer exposure to this dye. No detectable staining of the nucleus has been detected under these conditions. Electrophoretic studies of both undigested and restricted DNAs confirm that the nuclear DNA is unaffected by high levels of EB, with the exception of the rDNA-bearing chromosome that undergoes significant structural alterations in the presence of EB. Moreover, the hybridization signal with the rDNA probe is proportionally reduced in samples obtained from cultures grown in the presence of EB, suggesting that the average copy number of rRNA genes in these cultures may be affected. In striking contrast to other fungal species, the linear organelle genome in C. parapsilosis retains its structural and functional integrity in the presence of high concentrations of EB.

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“…The medium for the screening of respiratory-deficient mutants was prepared according to O'Connor et al (1976). The complete medium (GYP) contained (per litre) 10 g of Bacto peptone, 20 g of glucose, 1 g of KH 2 PO 4 and 1 g of MgSO 4 Á 7H 2 O. Glycerol medium (GlyYP) was made using 20 g of glycerol instead of 20 g of glucose in medium GYP.…”

Section: Mediamentioning

confidence: 99%

“…Cytochrome absorption spectra were analyzed according to the methods of De Deken (1966) andO'Connor et al (1976).…”

Section: Assay Of Key Enzymes In the Etcmentioning

confidence: 99%

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Significant increase of glycolytic flux inTorulopsis glabrataby inhibition of oxidative phosphorylation

Liu

et al. 2006

FEMS Yeast Research

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This study was aimed at increasing the glycolytic flux of the multivitamin-auxotrophic yeast Torulopsis glabrata by disturbing oxidative phosphorylation. We examined two different strategies to impede oxidative phosphorylation. The first strategy was disruption of the activity of the electron transfer chain (ETC), by either of two approaches. One was separately adding, at 10 mg L1, specific inhibitors of complex I (rotenone) or of the bc1 complex (antimycin A) to the culture broth of T. glabrata CCTCC M202019, which resulted in significantly decreased intracellular ATP levels (43% and 27.7%) and significantly increased rates of glucose consumption (qs) and pyruvate production (qp); another approach was breeding a respiratory-deficient mutant RD-16, in which cytochromes aa3 and b in the ETC were deleted after ethidium bromide mutagenesis, to reduce the ETC activity constitutively. The second strategy was inhibiting F0F1-ATP synthase with 0.05 mM oligomycin. Also, a neomycin-resistant mutant with 65% decreased F0F1-ATPase activity was studied. With the two strategies, the specific activity of phosphofructokinase (R2=0.9971), the average specific glucose consumption rate (R2=0.9967) and the average specific pyruvate production rate (R2=0.965) were closely correlated with the intracellular ATP level, all of them being increased at a lower intracellular ATP level.

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Respiratory-deficient mutants of Torulopsis glabrata, a yeast with circular mitochondrial deoxyribonucleic acid of 6 mu m

Abstract: and maintained on GYP slopes (see below). Media. The basic yeast extract, peptone medium (YP), contains per liter: 3 g of KH2PO4, 5 g of yeast 959

Cited by 47 publications

References 22 publications

Genome comparison in yeast systematics: delimitation of species within the genera Schwanniomyces, Saccharomyces, Debaryomyces, and Pichia.

Genome comparison in yeast systematics: delimitation of species within the genera Schwanniomyces, Saccharomyces, Debaryomyces, and Pichia.

The in vivo effects of ethidium bromide on mitochondrial and ribosomal DNA in Candida parapsilosis

Significant increase of glycolytic flux inTorulopsis glabrataby inhibition of oxidative phosphorylation

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