Respiratory complex I deficiency results in low nitric oxide levels, induction of hemoglobin and upregulation of fermentation pathways

Shah, Jash; Cochrane, Devin W.; Paepe, Rosine De; Igamberdiev, Abir U.

doi:10.1016/j.plaphy.2012.11.022

Cited by 21 publications

(14 citation statements)

References 42 publications

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“…), and was reported recently in Nicotiana sylvestris plants with an altered respiratory complex I (Shah et al . ). Usually, a decrease in the energy state of the tissue correlates well with fermentation induction under low‐oxygen conditions.…”

Section: Resultsmentioning

confidence: 97%

Both foliar and residual applications of herbicides that inhibit amino acid biosynthesis induce alternative respiration and aerobic fermentation in pea roots

et al. 2015

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The objective of this work was to ascertain whether there is a general pattern of carbon allocation and utilisation in plants following herbicide supply, independent of the site of application: sprayed on leaves or supplied to nutrient solution. The herbicides studied were the amino acid biosynthesis-inhibiting herbicides (ABIH): glyphosate, an inhibitor of aromatic amino acid biosynthesis, and imazamox, an inhibitor of branched-chain amino acid biosynthesis. All treated plants showed impaired carbon metabolism; carbohydrate accumulation was detected in both leaves and roots of the treated plants. The accumulation in roots was due to lack of use of available sugars as growth was arrested, which elicited soluble carbohydrate accumulation in the leaves due to a decrease in sink strength. Under aerobic conditions, ethanol fermentative metabolism was enhanced in roots of the treated plants. This fermentative response was not related to a change in total respiration rates or cytochrome respiratory capacity, but an increase in alternative oxidase capacity was detected. Pyruvate accumulation was detected after most of the herbicide treatments. These results demonstrate that both ABIH induce the less-efficient, ATP-producing pathways, namely fermentation and alternative respiration, by increasing the key metabolite, pyruvate. The plant response was similar not only for the two ABIH but also after foliar or residual application.

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Section: Resultsmentioning

confidence: 97%

Both foliar and residual applications of herbicides that inhibit amino acid biosynthesis induce alternative respiration and aerobic fermentation in pea roots

et al. 2015

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“…However, despite their significant increase in both CMSII and NMS1 mutants, absolute flux values were small (< 0.05 μmol m −2 s −1 in Fig. ; see also Shah et al ., ).…”

Section: Discussionmentioning

confidence: 97%

“…S10), with a considerable decrease in their phosphorylation (Abadie et al ., ). Indeed, complex I mutants exhibit a metabolic syndrome similar to hypoxia, with, for example, the synthesis of alanine, lactate and GABA (present results and Shah et al ., ). The effect on mitochondrial protein phosphorylation in mutants would be consistent with a decrease in mTOR (Mammalian Target of Rapamycin) activity, as already observed in mammalian cells (Schieke et al ., ).…”

Section: Discussionmentioning

confidence: 97%

Mitochondrial complex I dysfunction increases CO₂ efflux and reconfigures metabolic fluxes of day respiration in tobacco leaves

2018

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Mutants affected in complex I are useful to understand the role played by mitochondrial electron transport and redox metabolism in cellular homeostasis and signaling. However, their respiratory phenotype is incompletely described and a specific examination of day respiration (R ) is lacking. Here, we used isotopic methods and metabolomics to investigate the impact of complex I dysfunction on R in two respiratory mutants of forest tobacco (Nicotiana sylvestris): cytoplasmic male sterile II (CMSII) and nuclear male sterile 1 (NMS1), previously characterized for complex I disruption. R was higher in mutants and the inhibition of leaf respiration by light was lower. Higher R values were caused by increased (phosphoenol)pyruvate (PEP) metabolism at the expense of anaplerotic (PEP carboxylase (PEPc) -catalyzed) activity. De novo synthesis of Krebs cycle intermediates in the light was larger in mutants than in the wild-type, although numerically small in all genotypes. Carbon metabolism in mutants involved alternative pathways, such as alanine synthesis, and an increase in amino acid production with the notable exception of aspartate. Our results show that the alteration of NADH re-oxidation activity by complex I does not cause a general inhibition of catabolism, but rather a re-orchestration of fluxes in day respiratory metabolism, leading to an increased CO efflux.

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“…In this method the reaction between NO and ozone (O 3 ) generates nitrogen dioxide (NO * 2 ) in the excited-state which then emits a photon and reaches its ground state, a photomultiplier counts the light generated in the amount proportional to NO content. Chemiluminescence has been used to measure NO emissions from leaves, roots and from isolated mitochondria (Planchet et al, 2005; Gupta et al, 2011; Shah et al, 2013). This method is highly sensitive and can detect NO in the range of parts per billion which corresponds to picomolar concentrations in the tissue.…”

Section: Overview Of No Detection Methods: Advantages and Technical Pmentioning

confidence: 99%

Recommendations of using at least two different methods for measuring NO

Gupta¹,

Igamberdiev²

2013

Front. Plant Sci.

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Respiratory complex I deficiency results in low nitric oxide levels, induction of hemoglobin and upregulation of fermentation pathways

Cited by 21 publications

References 42 publications

Both foliar and residual applications of herbicides that inhibit amino acid biosynthesis induce alternative respiration and aerobic fermentation in pea roots

Both foliar and residual applications of herbicides that inhibit amino acid biosynthesis induce alternative respiration and aerobic fermentation in pea roots

Mitochondrial complex I dysfunction increases CO₂ efflux and reconfigures metabolic fluxes of day respiration in tobacco leaves

Recommendations of using at least two different methods for measuring NO

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Respiratory complex I deficiency results in low nitric oxide levels, induction of hemoglobin and upregulation of fermentation pathways

Cited by 21 publications

References 42 publications

Both foliar and residual applications of herbicides that inhibit amino acid biosynthesis induce alternative respiration and aerobic fermentation in pea roots

Both foliar and residual applications of herbicides that inhibit amino acid biosynthesis induce alternative respiration and aerobic fermentation in pea roots

Mitochondrial complex I dysfunction increases CO2 efflux and reconfigures metabolic fluxes of day respiration in tobacco leaves

Recommendations of using at least two different methods for measuring NO

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Mitochondrial complex I dysfunction increases CO₂ efflux and reconfigures metabolic fluxes of day respiration in tobacco leaves