Respiratory burst response of peritoneal leukocytes adhering to titanium and stainless steel

Abstract

Titanium sheets, made hydrophilic by oxidative cleaning or hydrophobic by treatment with butanol, and stainless steel sheets with different patterns of pores (straight phi = 0.8 mm) were implanted into the peritoneal cavity of mice. The implants were removed after 2 h, and the surface-adhering leukocytes were stained with propidium iodide and fluorescein diacetate to quantitate cell adhesion and to indicate the presence of leaks in the cell membrane. The ability of the surface-adhering leukocytes to mount a respiratory burst response after stimulation with PMA or zymosan was measured by chemiluminiscence. The results show that stainless steel without pores induces membrane leakage in 80% of the surface-adhering leukocytes compared with 65% of cells adhering to porous steel. Hydrophilic titanium induces membrane leakage in 48% of the surface-adhering leukocytes compared with 19% of cells adhering to hydrophobic titanium. The respiratory burst response of the surface-adhering leukocytes stimulated with PMA was attenuated on stainless steel and hydrophilic titanium compared with hydrophobic titanium. Thus, butanol treatment of titanium and pores in stainless steel increase the biocompatibility of the materials.

Free Radical Production in Immune Cell Systems Induced by Ti, Ti6Al4V and SS Assessed by Chemiluminescence Probe Pholasin Assay

“…Cells adherent to titanium were not active but have the ability to respond to a microorganism when challenged with the correct stimuli. In another work by Nygren et al [29], titanium and stainless steel sheets implanted in vivo were analysed by chemiluminescence and the ability of the surface-adhering leukocytes to mount a respiratory burst after stimulation with PMA or zymosan was measured. The results from their study showed higher ROS production for stainless steel when challenged with opsonised zymosan when compared with titanium or PMA challenge.…”

Adhesion of human leukocytes to biomaterials: An in vitro study using alkanethiolate monolayers with different chemically functionalized surfaces

“…Lectins are proteins of non‐immune origin that agglutinate cells and/or precipitate complex carbohydrates. These activators have been used in several studies to assess the response of activated blood cells to biomaterials 21, 23–27…”

Fabrication of Arrays of Polymer Gradients Using Inkjet Printing

“…In this case, the amount and distribution of adsorbed proteins depends on the artificial surface and has an impact on platelet adhesion. 212,213 In the second step of platelet activation, self-association of platelets into a plug can be observed -the so called platelet aggregation step. Thereby, fibrinogen interacts with the fibrinogen receptor complex gpIIb/IIIa on the platelet surface.…”