Respiratory activity of beef thyroid mitochondria

Gonze, Jeanine; Tyler, D.D.

doi:10.1016/0006-291x(65)90120-8

Cited by 5 publications

(1 citation statement)

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“…More recently, phosphorylating mitochondria have been isolated from thyroid homogenates (Turkington & Nordwind, 1962). These preparations contain the phosphorylating respiratory-chain system present in other types of mammalian mitochondria, and oligomycin, antimycin and rotenone have been found to exert their usual effects on the electronand energy-transfer reactions (Lamy, Rodesch & Dumont, 1964; Gonze & Tyler, 1965;Degroot, Dunn & Jaksina, 1966).…”

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confidence: 99%

Influence of mitochondrial inhibitors on the respiration and energy-dependent uptake of iodide by thyroid slices

Tyler¹,

Gonze²,

Lamy³

et al. 1968

Biochemical Journal

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The influence of mitochondrial inhibitors, including oligomycin, antimycin and rotenone, on the iodide and oxygen uptake and the nucleotide content of incubated sheep thyroid slices was investigated. Each inhibitor strongly suppressed both iodide and oxygen uptake, and decreased the nucleoside triphosphate content of the slices. In most cases the addition of glucose or mitochondrial substrates restored iodide uptake in inhibitor-treated slices. Inhibitor concentrations sufficient to inhibit iodide uptake strongly had only slight effects on the thyroidal Na(+)+K(+)-activated adenosine triphosphatase. It is concluded that the inhibitors produce their effects by the inhibition in vivo of mitochondrial oxidative phosphorylation. ATP synthesis appears to be essential for iodide uptake to occur, and the high-energy intermediates (or energized state) of oxidative phosphorylation cannot be used to energize the uptake process. To a limited extent glycolytic ATP synthesis can support iodide uptake, which is therefore not exclusively dependent on aerobic metabolism. The mechanism of energy-linked iodide uptake is discussed.

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confidence: 99%