Respiration of steelhead trout sperm: sensitivity to pH and carbon dioxide

Ingermann, Rolf L.; Robinson, Mary Lou; Cloud, J. G.

doi:10.1046/j.1095-8649.2003.00003.x

Cited by 32 publications

(28 citation statements)

References 29 publications

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“…The inhibition of motility of sperm in semen is mainly due to osmotic pressure in most species (Morisawa and Suzuki 1980, Stoss 1983, Linhart et al 1991, Billard et al 1995a) but K þ plays a major role in salmonids (Schlenk and Kahmman 1938, Stoss 1983, Billard et al 1995b) and in sturgeons (Gallis et al 1991, Billard 2000. Several parameters of the swimming medium, such as ion concentration (K þ , Na þ , Ca 2þ , Mg 2þ ), osmotic level, pH and dilution rate, affect the motility duration of fish spermatozoa (Morisawa and Suzuki 1980, Stoss 1983, Linhart et al 1991, Billard et al 1995b, Tvedt et al 2001, Ingermann et al 2003. Optimum sperm motility was observed in alkaline pH and with a low dilution rate (at 1:50) in Acipenseridae (Gallis et al 1991, Alavi et al 2002.…”

Section: Introductionmentioning

confidence: 99%

Spermatozoa motility in the Persian sturgeon, Acipenser persicus: effects of pH, dilution rate, ions and osmolality

Alavi¹,

Cosson²,

Karami³

et al. 2004

Reproduction

102

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Sperm motility is a prerequisite factor determining semen quality and fertilizing capacity. The effects of environmental factors including pH, cations and osmolality as well as the role of dilution rate on sperm motility parameters in Acipenser persicus were studied. The best pH and dilution rate for activation of spermatozoa were pH 8.0 and dilution ratio 1:50. Ionic factors can stimulate the initiation of sperm activation. The maximum percentage of motile sperm and total duration of sperm motility were observed in solutions containing 25 mM NaCl, 0.2 mM KCl, 3 mM CaSO 4 , 10 mM MgSO 4 and sucrose with an osmolality of 50 mosmol kg 21. The present study provides us with some basic knowledge about sturgeon spermatozoa biosensitivity to ionic and osmolality effects. A sensitivity of A. persicus sperm was observed after induction of activation of sperm motility in solution containing cations or sucrose with high osmolality. Concentrations more than 50 mM Na 1 , more than 1 mM K 1 , more than 3 mM Ca 21 and more than 10 mM Mg 21 had negative effects on sperm motility. Also, osmolality more than 100 mosmol kg 21 had an inhibitory effect. It is clear that ions and osmolality stimulate the motility of spermatozoa by changes in the properties of the plasma membrane including its potential and its ionic conductance. The inhibitory role of high osmolality of the swimming medium (more than 100 mosmol kg 21 ) and insufficient osmolality of the seminal plasma to inhibit semen motility suggested that osmolality is not the principal factor preventing sperm motility in seminal fluid but that K 1 is a major inhibitory factor of sperm motility in seminal plasma.

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Section: Introductionmentioning

confidence: 99%

Spermatozoa motility in the Persian sturgeon, Acipenser persicus: effects of pH, dilution rate, ions and osmolality

Alavi¹,

Cosson²,

Karami³

et al. 2004

Reproduction

102

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“…External pH in a wide range does not influence freshwater fish sperm motility (Alavi and Cosson, 2005). On the other hand, acidification of extracellular environments suppresses sperm motility in white sturgeon and trout (Ingermann et al, 2002(Ingermann et al, , 2003Alavi and Cosson, 2005). It is possible that sperm motility initiation regulation in A. burtoni accommodates different ionic environments of lake and river water with respect to Na 1 and K 1 , but not H 1 .…”

Section: Discussionmentioning

confidence: 95%

“…In contrast, the rivers that flow into the lake are acidic (pH 6.5). It has been reported that pH impacts have little effect on sperm motility activation (Ingermann et al, 2002(Ingermann et al, , 2003Alavi and Cosson, 2005). Among Tanganyikan cichlids, A. burtoni can inhabit and reproduce in both the lake and rivers.…”

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confidence: 97%

Sperm motility adaptation to ion‐differing aquatic environments in the Tanganyikan cichlid, Astatotilapia burtoni

et al. 2010

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The cichlid fish, Astatotilapia burtoni, can acclimate and reproduce in both the K(+)-, Na(+)-, and Ca(2+)-rich waters of Lake Tanganyika (pH 8.9-9.2) and the surrounding rivers where K(+), Na(+), and Ca(2+) concentrations are low (pH 6.5). In this study, we compared sperm motility features among A. burtoni inhabiting the lake, those living in the surrounding rivers, and those from rivers that acclimates to lake water. Sperm of all three groups required extracellular Ca(2+) ([Ca(2+)]o) for sperm motility activation. However, involvement of K(+) and Na(+) were different. In sperm motility initiation of lake population, the decrease in Na(+) and increase in K(+) concentrations corresponding to a decrease in [Na(+)]o/[K(+)]o was required. In contrast, sperm motility in both the river population and those acclimated to lake water were activated only by a decrease in [Na(+)]o, suggesting that motility initiation cues regarding Na(+) and K(+) are different among populations within same species. One probable reason why the difference of initiation cues is that the concentration of K(+) in the river water (40 mM) is lower than that in the lake water (110 mM). These results suggest that sperm flagellar activation cascades of river and lake populations within this species differ as a result of adaptations to different ionic environments.

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“…glycine, glucose or lactate plus pyruvate) the lactate plus pyruvate seems to be the most favourable to maintain ATP concentration. ATP level in spermatozoa of different species have been positively correlated with good motility (Ingermann et al 2003). It means that good ATP level is strictly related to good motility and finally to better fertilising potential.…”

Section: Discussionmentioning

confidence: 98%

ATP Content and Adenine Nucleotide Catabolism in African Catfish Spermatozoa Stored in Various Energetic Substrates

Ziętara

Słomińska

Świerczyński

et al. 2004

Fish Physiol Biochem

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Influence of various energetic substrates on ATP content and adenine nucleotide catabolism in African catfish, Clarias gariepinus spermatozoa was investigated. Present data indicate that sperm storage at 4°C in the presence of different energetic substrates leads to time dependent ATP depletion. Among the substrates studied (i.e. glycine, glucose, or lactate plus pyruvate), the lactate plus pyruvate seems to be the most favourable substrate to maintain ATP concentration. ATP depletion is associated with increase of the AMP content, while ADP concentration is changing only slightly. Moreover, significant increase in hypoxanthine content both in the sperm and a storage medium was found. Adenine nucleotide energy charge (AEC) decreased significantly during the first 24 h when sperm was stored in the presence of glycine. During the same period, only slight decrease in AEC was observed in spermatozoa stored in the presence of glucose and no significant changes were found in the presence of lactate plus pyruvate. A further decrease in AEC was observed until 120 h of storage in the medium containing glycine, lactate plus pyruvate or glucose. However, the changes were relatively small when spermatozoa were stored in the presence of lactate plus pyruvate. Important finding is that in contrast to the results reported recently, AEC value in fresh milt (at 0 time of storage) was in the normal range. The present study shows that African catfish spermatozoa are able to maintain physiological level of ATP and AEC when they are stored in the presence of favourable energetic substrates. In conclusion, the results of this investigation suggest that successful short-term storage of African catfish milt depends on energetic substrates supply.Abbreviations: AEC -adenine nucleotide energy charge; ADP -adenosine diphosphate; AMP -adenosine monophosphate; ATP -adenosine triphosphate; cAMP -cyclic AMP; HPLC -high performance liquid chromatography; IMP -inosine monophosphate; TAN -total adenine nucleotide.

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Respiration of steelhead trout sperm: sensitivity to pH and carbon dioxide

Cited by 32 publications

References 29 publications

Spermatozoa motility in the Persian sturgeon, Acipenser persicus: effects of pH, dilution rate, ions and osmolality

Spermatozoa motility in the Persian sturgeon, Acipenser persicus: effects of pH, dilution rate, ions and osmolality

Sperm motility adaptation to ion‐differing aquatic environments in the Tanganyikan cichlid, Astatotilapia burtoni

ATP Content and Adenine Nucleotide Catabolism in African Catfish Spermatozoa Stored in Various Energetic Substrates

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