Respiration of homogenates and crude mitochondrial fractions of bovine attached gingiva

Respiration of bovine gingival epithelium Action of diphenylhydantoin

“…The endogenous oxygen consumption by bovine gingival epithelium slices presented in this paper is low compared with the results showed by Lainson & Fisher (1966), Morgan & Wingo (1966) and Rosett et al (1972). The values reported by these authors were derived by techniques somewhat different from those used in the present study and this may account for the difference-Concerning the intermediates of Krebs cycle, only succinate and malate were oxidized by bovine gingival epithelium slices-…”

“…The endogenous oxygen consumption by bovine gingival epithelium mitochondrial fraction had a QO2 of 4-40 and 3.00, respectively. There have been numerous studies dealing with respriration of gingival tissues; apparently only Rosett et al-(1972) worked with mitochondrial fraction. A comparison between the results presented here with that of Rosett et al (1972) is very difficult since the authors did not determined the amount of protein per Warburg flask.…”

Preparation of Rat Gingival Mitochondria with an Improved Isolation Method

“…However, neither the damage to fibroblasts in Mt disease [16] nor the effect of nicotine on Mt in periodontal tissues [17] has ever been reportedly investigated by the measurement of respiratory activity. Moreover, Rosett et al [2] have suggested that it is impossible to prepare Mt from attached gingival tissue, because of the presence of collagen, which disturbs the isolation of Mt. In the present study of gingival Mt preparation, the changes of the duration of the homogenization time and the concentration of collagenase were investigated.…”

“…There are a few reports in the literature on the preparation of mitochondria (Mt) from epithelial tissue for the purpose of the direct measurement of respiratory activity [2–7, 16, 17]. However, neither the damage to fibroblasts in Mt disease [16] nor the effect of nicotine on Mt in periodontal tissues [17] has ever been reportedly investigated by the measurement of respiratory activity.…”

“…However, oxidative phosphorylation (ATP synthesis) and electron transport in oral tissues, for example, the oral mucosa and gingival tissue, were extensively studied in a period of the late 1970s to early 1980s by several investigators [2–8]. Mitochondria in the oral tissues of animals and humans have been isolated by differential centrifugation of tissue homogenates with or without collagenase treatment [2–5]. Moreover, to avoid the destruction of mitochondria or the loss of respiratory activity, chilled buffer solutions of an isotonic tension have generally been adopted.…”