Respiration: comparison of the Winkler technique, O2 electrodes, O2 optodes and the respiratory electron transport system assay

Bondyale-Juez, Daniel R.; Packard, Theodore T.; Viera-Rodríguez, María Ascensión

doi:10.1007/s00227-017-3271-1

Cited by 11 publications

(5 citation statements)

References 70 publications

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“…As a result, many methods have been developed to assess oxygen consumption by heterotrophs or production by photosynthetic microbes (see review in Renger and Hanssum, 2009 ). Indeed, oxygen concentration can be measured in solution using electrodes (electrochemical sensors), optodes (optical-based sensors), or chemical assays such as the Winkler titration or directly in the headspace of sealed vials using laser spectrometry ( Bondyale-Juez et al, 2017 ).…”

Section: Electron Acceptor Consumption Ratementioning

confidence: 99%

“…Therefore, the Winkler titration is difficult to apply serially to assess the rapid consumption of oxygen. As a result, optical and electrochemical sensors have been preferred in many cases ( Kemker, 2014 ; Bondyale-Juez et al, 2017 ), especially as some systems have been introduced as 96-well plates ( Guarino et al, 2004 ; Dike et al, 2005 ). Nowadays, both optical- and electrochemical-based sensors have very good performance and can sense dissolved oxygen in an aqueous system easily and accurately, usually with an error below 1% ( Briand et al, 2004 ; Tengberg et al, 2006 ; Renger and Hanssum, 2009 ).…”

Section: Electron Acceptor Consumption Ratementioning

confidence: 99%

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A Review of Methods to Determine Viability, Vitality, and Metabolic Rates in Microbiology

et al. 2020

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Viability and metabolic assays are commonly used as proxies to assess the overall metabolism of microorganisms. The variety of these assays combined with little information provided by some assay kits or online protocols often leads to mistakes or poor interpretation of the results. In addition, the use of some of these assays is restricted to simple systems (mostly pure cultures), and care must be taken in their application to environmental samples. In this review, the necessary data are compiled to understand the reactions or measurements performed in many of the assays commonly used in various aspects of microbiology. Also, their relationships to each other, as metabolism links many of these assays, resulting in correlations between measured values and parameters, are discussed. Finally, the limitations of these assays are discussed.

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Section: Electron Acceptor Consumption Ratementioning

confidence: 99%

Section: Electron Acceptor Consumption Ratementioning

confidence: 99%

A Review of Methods to Determine Viability, Vitality, and Metabolic Rates in Microbiology

et al. 2020

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“…In principle, there is a number of conventional techniques capable of determination of highly electronegative nonmetals in a liquid phase, including spectrometry (Wang & Wolfbeis, 2014), the use of CNO‐analysers (Kirsten, 1983), Winkler's titration (Bondyale‐Juez et al, 2017; Shriwastav et al, 2017), pyrohydrolysis (Banks, Burke, & O'Laughlin, 1958; Shekhar et al, 2003), ion chromatography (Kapinus et al, 2004; Sawant et al, 2007; Zhang et al, 2012; Mukai et al, 2019; Wang et al, 2020), high‐performance liquid chromatography (Xu et al, 2004), and electrochemical approaches (ion‐selective electrodes and voltammetry) (Lupo et al, 2012; Mao et al, 2013; Bektas et al, 2018). In the case of analyzing solids, those techniques are limited by the aforementioned dissolution problems.…”

Section: Introductionmentioning

confidence: 99%

Mass Spectrometry‐based Techniques for Direct Quantification of High Ionization Energy Elements in Solid Materials—challenges and Perspectives

Gubal

Chuchina

Sorokina

et al. 2020

Mass Spectrometry Reviews

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The determination of nonmetals, first of all, the most electronegative ones—nitrogen, oxygen, fluorine, chlorine, and bromine, poses the highest challenge for element analysis. These elements are characterized by high reactivity, volatility, high ionization energy, and the absence of intensive spectral lines in the optical spectral range. Conventional techniques of their quantification include considerable “wet chemistry” stages so the application of these techniques for the solid sample is highly laborious and prone to uncontrollable uncertainties. Additionally, current development in material science and other areas requires the quantification of the elements at lower levels with good sensitivity. Owing to their robustness and flexibility, mass spectrometry techniques provide vast possibilities for the quantification, spatial and isotopic analysis, including the solutions for direct analysis of solids. The current review focuses on the application of major mass spectrometric techniques for the quantification of N, O, F, Cl, and Br in solid samples. The following techniques are mainly considered: thermal ionization mass spectrometry (TIMS), isotope‐ratio MS (IRMS), secondary ion MS (SIMS), inductively coupled plasma MS (ICP‐MS), and glow discharge MS (GDMS); as the most accessible and widely applied for the purpose. General ionization issues, advantages, limitations, and novel methodological solutions are discussed. © 2020 John Wiley & Sons Ltd.

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“…In the case of V. velella, the presence of endosymbiotic photosynthetic dinoflagellates (Blank and Trench, 1986) may allow it to survive without prey and live with an even lower R: ratio (Bondyale-Juez et al, 2017). However, we realize that our measurements need confirmation and replication in order to strengthen them (Schmidt, 2009).…”

Section: Respiratory Metabolismmentioning

confidence: 88%

“…These upside-down jellyfish have a more benthic lifestyle with a strong reliance for food on its symbiotic dinoflagellate (Lampert, 2016). Hence, their pulsation is less locomotive and the presence of these autotrophs may increase the ETS activity measured, leading to a higher (Bondyale-Juez et al, 2017). A low R: (0.19 ± 0.10) was also found in sessile polyps of A. aurita (Purcell et al, 2019).…”

Section: Respiratory Metabolismmentioning

confidence: 96%

Wind Drifting vs. Pulsating Swimming Jellyfish: Respiratory Metabolism and Composition Differences in Physalis physalis, Velella velella, Aurelia aurita, and Pelagia noctiluca

et al. 2022

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Physalia physalis and Velella velella, are among the few marine organisms that harness the wind for their locomotion, whereas other cnidarian jellyfish make use of their pulsating bell-shaped bodies to propel themselves through the seas. We investigate their composition and metabolism compared with two species of pulsating scyphozoan jellyfish, Aurelia aurita and Pelagia noctiluca. Protein (P), lipid (L), carbohydrate (K), and derived energy content (Ec), provided information on the biochemical composition of these species and their relevance as prey. Physiological respiration (R) from oxygen consumption. As well as potential respiration (Φ) from the electron transport system (ETS) activity and the derived respiratory carbon demand (RCD) and heterotrophic energy transformation (HET), allow the comparison of the impact of these two types of propulsion on the metabolism, along with the impact of these organisms as predators. In this study it was found that these hydrozoans depicted a different biochemical composition relative to other gelatinous zooplankton. Lower water content at around 90% was observed, while WM-specific P, L, K, and Ec were higher, showcasing new aspects of these species as prey. The lower R/P in P. physalis and V. velella (1.8 ± 0.7 and 2.9 ± 1.1 μL O2 h–1 mg Prot–1, respectively) and the low R/Φ, around 0.1, indicate lower respiration in wind-driven propulsion compared to pulsation-driven propulsion. Additionally, these results encourage the use and research on enzymatic techniques that are particularly useful for gelatinous research, and the calculation of RCD and HET helps in understanding the physiology and role played by the organisms as predators from carbon and energy perspectives.

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Respiration: comparison of the Winkler technique, O2 electrodes, O2 optodes and the respiratory electron transport system assay

Cited by 11 publications

References 70 publications

A Review of Methods to Determine Viability, Vitality, and Metabolic Rates in Microbiology

A Review of Methods to Determine Viability, Vitality, and Metabolic Rates in Microbiology

Mass Spectrometry‐based Techniques for Direct Quantification of High Ionization Energy Elements in Solid Materials—challenges and Perspectives

Wind Drifting vs. Pulsating Swimming Jellyfish: Respiratory Metabolism and Composition Differences in Physalis physalis, Velella velella, Aurelia aurita, and Pelagia noctiluca

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