RespiFinder: a New Multiparameter Test To Differentially Identify Fifteen Respiratory Viruses

Reijans, Martin; Dingemans, Gijs; Klaassen, Corné H. W.; Meis, Jacques F.; Keijdener, Judith; Mulders, Brit; Eadie, Kimberly; Leeuwen, Willem van; Belkum, Alex van; Horrevorts, A. M.; Simons, Guus

doi:10.1128/jcm.02294-07

Cited by 118 publications

(108 citation statements)

References 35 publications

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“…The assay is designed to detect up to 15 different agents in a single sample and has a very high specificity (99.2% for HMPV). An alternative multiplex detection method for respiratory pathogens is the RespiFinder technology (Pathofinder, Maastricht, The Netherlands), which is based on multiplex PCRs that are analyzed by subsequent capillary gel electrophoresis (192). This technology make use of multiplex ligation-dependent probe amplification (MLPA) and employs two probes which ligate exclusively in the presence of target-specific complementary sequences (34,192).…”

Section: Diagnostics Molecular Diagnosticsmentioning

confidence: 99%

“…An alternative multiplex detection method for respiratory pathogens is the RespiFinder technology (Pathofinder, Maastricht, The Netherlands), which is based on multiplex PCRs that are analyzed by subsequent capillary gel electrophoresis (192). This technology make use of multiplex ligation-dependent probe amplification (MLPA) and employs two probes which ligate exclusively in the presence of target-specific complementary sequences (34,192). This assay was shown to have high sensitivity (98.2%) and specificity (100%) for HMPV in a study that investigated 144 clinical samples (192).…”

Section: Diagnostics Molecular Diagnosticsmentioning

confidence: 99%

See 1 more Smart Citation

Human Metapneumovirus: Lessons Learned over the First Decade

Schildgen

Hoogen²,

Fouchier³

et al. 2011

Clin Microbiol Rev

181

187

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SUMMARY It has been 10 years since human metapneumovirus (HMPV) was identified as a causative agent of respiratory illness in humans. Since then, numerous studies have contributed to a substantial body of knowledge on many aspects of HMPV. This review summarizes our current knowledge on HMPV, HMPV disease pathogenesis, and disease intervention strategies and identifies a number of areas with key questions to be addressed in the future.

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Section: Diagnostics Molecular Diagnosticsmentioning

confidence: 99%

Section: Diagnostics Molecular Diagnosticsmentioning

confidence: 99%

Human Metapneumovirus: Lessons Learned over the First Decade

Schildgen

Hoogen²,

Fouchier³

et al. 2011

Clin Microbiol Rev

181

187

View full text Add to dashboard Cite

show abstract

“…MLPA technology was shown to allow amplification of up to 40 different targets simultaneously through the use of one universal primer set in the final amplification (12). Recently, the MLPA technology was applied for the first time for detection of infectious agents causing respiratory tract infections (11). The goal of the current study was to design and evaluate the MeningoFinder assay ( Fig.…”

mentioning

confidence: 99%

“…Viral RNA/DNA from clinical samples (200-l cerebrospinal fluid specimens) for PCR or MLPA was isolated using either the Nuclisense easyMAG (bioMerieux, Boxtel, The Netherlands) or the MagnaPure LC nucleic acid system (Roche Diagnostics) according to the manufacturers' instructions. Before starting the extraction, a competitive internal control (IAC) was added; IAC was constructed as described previously (11). The MeningoFinder probes were designed against conserved viral genomic regions, i.e., the CMV UL89 gene (gb DQ48009.1, positions 1767 to 1824), the EBV nuclear antigen 1 gene (gb AY825078.1, positions 280 to 346), the enterovirus polyprotein gene (emb AJ295206.1 HEC295206, positions 469 to 525), the HSV-1 and HSV-2 US6 genes (gi 30961596 gb AY240830.1, positions 89 to 155, and gb EU018091.1, positions 154 to 220) and the VZV immediate-early 62 gene (gb AY253719.1, positions 1996 to 2062).…”

mentioning

confidence: 99%

Evaluation of MeningoFinder, a Novel Multiplex Ligation-Dependent Probe Amplification Assay for Simultaneous Detection of Six Virus Species Causing Central Nervous System Infections

et al. 2009

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“…The different HPV typing methods that were used to generate results for the WHO LabNet proficiency study to detect HPV DNA (11,(16)(17)(18)(19)(20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30)(31)(32)(33)(34) are summarized in Table 2.…”

mentioning

confidence: 99%

Global Improvement in Genotyping of Human Papillomavirus DNA: the 2011 HPV LabNet International Proficiency Study

Eklund

Forslund

Wallin³

et al. 2014

J Clin Microbiol

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Cervical cancer is the third most common cancer among women worldwide, with an estimated 530,000 new cases diagnosed annually. Human papillomavirus (HPV) infection is linked to Ͼ99% of cervical cancers (1). The most important highrisk types (HPV16 and HPV18) account for about 70% of all invasive cervical cancers worldwide (2).The introduction of HPV vaccines has highlighted the need for accurate and internationally comparable HPV DNA detection and genotyping methodologies. This is an essential component in both the development and evaluation of HPV vaccines, as well as in the effective implementation and monitoring of HPV vaccination programs. The genotyping assays used today differ in their analytical performances with regard to type-specific sensitivities and specificities. Several studies have compared different HPV typing assays to assess their performances in screening and epidemiology using clinical samples (3-5). However, evaluations of assay performance in different laboratories need to be performed in a standardized manner, such that different assay performance measures can be evaluated and the results can be compared over time against a known and accepted standard (4). The use of regularly issued global proficiency studies is an essential tool for establishing comparable and reliable laboratory services (6). International proficiency panels for quality assurance of laboratory testing are being widely distributed for a number of infectious agents. Recently, the World Health Organization (WHO) issued new reference genotype panels for both parvovirus B19 and hepatitis B virus (7,8).With the objective of facilitating the development and implementation of HPV vaccines by improving and standardizing the quality of HPV laboratory services, the WHO established a global HPV laboratory network (HPV LabNet) in 2005. The main activities within the HPV LabNet were the harmonization and standardization of the laboratory procedures used for HPV vaccine research and HPV vaccination program impact monitoring by the development of internationally comparable quality assurance methods, international standards, and reference reagents, as well as a laboratory manual for vaccinology (9-12). In 2008, recombinant HPV DNA plasmids were used to establish international standards (ISs) for HPV16 and HPV18 DNA, with an assigned potency in international units (IU) (13). That same year, WHO HPV LabNet conducted the first proficiency study that was open for participation from laboratories worldwide based on HPV DNA plasmids containing the genomes of 14 oncogenic and 2 benign HPV types (12,14). In 2010, the proficiency study was repeated, demonstrating that it is possible to perform continuous global studies based on plasmid DNA with unitage traceable to ISs, and that such studies can provide an overview of the status of HPV detection and typing methodologies worldwide (6). The international HPV LabNet proficiency study described herein was designed for the genotyping needs in HPV vaccinology, and the proficiency criteria are not inten...

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RespiFinder: a New Multiparameter Test To Differentially Identify Fifteen Respiratory Viruses

Cited by 118 publications

References 35 publications

Human Metapneumovirus: Lessons Learned over the First Decade

Human Metapneumovirus: Lessons Learned over the First Decade

Evaluation of MeningoFinder, a Novel Multiplex Ligation-Dependent Probe Amplification Assay for Simultaneous Detection of Six Virus Species Causing Central Nervous System Infections

Global Improvement in Genotyping of Human Papillomavirus DNA: the 2011 HPV LabNet International Proficiency Study

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