Resorption und Einbau radioaktiv markierter Aminosäuren bei Verabreichung unterschiedlicher Proteinträger an Ratten

Abstract

Male Albino rats (90-100 g) were fed ad libitum (with limited periods of feeding) for 14 days. The diets were adjusted to a crude protein content of 10%. Powdered whole egg, fish meal, yeast and gelatine were used as protein sources. Additionally, one group of rats was fed a protein-free diet. On the 15th day of experiment the rats were fed a test diet at a level of 2 g per 100 g of body weight. 2 hrs after that the rats received 25 muCi of 3H glycine and 5 muCi of 14C-L-Leucine per 100 g of body weight administered by way of intragastric infusion. It was found that a large proportion of the radioactive amino acids were absorbed as early as after 0.5 hr. The highest rate of absorption was observed in animals fed dietary proteins of poor quality or a protein free diet, so that in animals receiving a gelatine diet or a protein-free diet only 68.4% or 56.4% of the administered amount of 14C activity were detected inside the gastro intestinal tract after 0.5 hr. Analogous data for the 3H activity were 52.4% and 25.3%. Maximum absorption occurred after 3-7 hrs. Following this the level of radioactivity in the intestinal contents again increased reaching a peak value after 14-24 hrs; in the case of 14C activity this peak value amounted to 25.4% of the administered dose in animals fed the gelatine diet and 32.8% in the group receiving the protein-free diet. It was established that the major proportion of the resecreted amount of 14C activity was present in leucine. Until 72 hrs after the intake of 14C activity the level of radioactivity was again found to decline, a processes which was induced by processes occurring in the large intestines. Moreover, evidence was obtained in confirmation of previous findings, indicating that the composition of faecal amino acids was constant and unaffected by dietary proteins.

Methodische Untersuchungen zur Verwendung von [14C]‐Leucin und [15N]‐Leucin zur Ermittlung des Resorptionsverlaufes von Aminosäuren bei der Versuchsratte

“…Die Verabreichung des 14C-marherten Leucins in Form einer Mageninfusion (2 h nach Aufnahme von 2 g Diat) in unseren friiheren Untersuchungen [7] fuhrte zu einer analogen , Abb. 1.…”