Resorption time and tissue reactions with magnesium rods in rats and rabbits

Hussl, H.; Papp, Christoph; Höpfel-Kreiner, I; Rumpl, E.; Wilflingseder, P

doi:10.1007/bf00289636

Cited by 13 publications

(4 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Mg implants have not previously been used to bridge and repair nerve gaps as described in this study, although one earlier study described inserting an Mg filament in a rabbit nerve. 29 Research on Mg implants in general is hampered by the fact that resorption of Mg in cell culture systems or in vitro in aqueous solutions occurs much faster than resorption in vivo. 30 Similarly, we observed little resorption after 6 weeks in vivo, while nothing remains intact past 1 week when similar wires are placed in cell culture medium (our lab, data not shown).…”

Section: Discussionmentioning

confidence: 99%

Initial observations on using magnesium metal in peripheral nerve repair

et al. 2014

View full text Add to dashboard Cite

Biodegradable magnesium metal filaments placed inside biodegradable nerve conduits might provide the physical guidance support needed to improve the rate and extent of regeneration of peripheral nerves across injury gaps. In this study, we examined basic issues of magnesium metal resorption and biocompatibility by repairing sub-critical size gap injuries (6 mm) in one sciatic nerve of 24 adult male Lewis rats. Separated nerve stumps were connected with poly(caprolactone) nerve conduits, with and without magnesium filaments (0.25 mm diameter, 10 mm length), with two different conduit filler substances (saline and keratin hydrogel). At 6 weeks after implantation, magnesium degradation was examined by micro-computed tomography and histological analyses. Magnesium degradation was significantly greater when the conduits were filled with an acidic keratin hydrogel than with saline (p < 0.05). But magnesium filaments in some animals remained intact for 6 weeks. Using histological and immunocytochemical analyses, good biocompatibility of the magnesium implants was observed at 6 weeks, as shown by good development of regenerating nerve mini-fascicles and only mild inflammation in tissues even after complete degradation of the magnesium. Nerve regeneration was not interrupted by complete magnesium degradation. An initial functional evaluation, determination of size recovery of the gastrocnemius muscle, showed a slight improvement due to magnesium with the saline but not the keratin filler, compared with respective control conduits without magnesium. These results suggest that magnesium filament implants have the potential to improve repair of injured peripheral nerve defects in this rodent model.

show abstract

Section: Discussionmentioning

confidence: 99%

Initial observations on using magnesium metal in peripheral nerve repair

et al. 2014

View full text Add to dashboard Cite

show abstract

“…The assessment of in vivo biocompatibility of Mg alloys in a soft tissue environment is rare, with very few articles available for the comparison of results. The majority of those that have been performed rely on brief descriptive results of the histology to indicate the tissue reaction to the materials [13,[48][49][50]. One study however, has thoroughly assessed the reaction of muscle tissue to a Mg-0.8Ca implant, using a semi-quantitative scale to assess fibrous encapsulation, tissue cavity formation, necrosis and the infiltration of macrophages, giant cells, heterophilic granulocytes, B-lymphocytes and T-lymphoctyes [51].…”

Section: Comparison Between the In Vivo Biocompatibility Of Mg Alloysmentioning

confidence: 99%

The in vitro and in vivo evaluation of the biocompatibility of Mg alloys

et al. 2013

View full text Add to dashboard Cite

Magnesium (Mg) and its alloys are being widely investigated for their potential use as resorbable biomaterials for orthopaedic applications. However, the natural corrosion of the metals results in potentially harmful perturbations to the physiological environment, which requires a comprehensive understanding of their biocompatibility. Currently, most investigations proceed directly from in vitro biocompatibility studies to intraosseous implantation. However, this can result in the unnecessary elimination of appropriate materials due to over sensitive in vitro methods or the implantation of potentially harmful materials. This study involved the development of a relevant in vitro cell culture method, and an in vivo soft tissue implantation technique to provide an intermediate step between basic cell culture methods and large animal intraosseous investigations. A Live/Dead fluorescent assay was used to investigate the viability of both L929 and SaOS-2 cells exposed to Mg alloys, with the results compared to those seen with the intramuscular implantation of the same materials in Lewis rats. These methods were able to successfully provide data on the corrosion of Mg alloys, allowing the identification of slowly and safely corroding materials that may be used in future intraosseous investigations.

show abstract

“…To investigate the safety of Mg implants, they implanted subcutaneously and intramuscularly pure Mg wires (99.8%) with a diameter of 0.25 and 0.50 mm in rats and rabbits [44]. They found that the Mg wires were totally absorbed within 20 weeks under extended hydrogen gas cavity formation, but the wires corroded even faster if pretreated with 10% acetic acid before implantation.…”

Section: Nerve and Muscle Tissuementioning

confidence: 98%

“…In 1981, the research group of the plastic surgeon Dr. Wilflingseder in Insbruck, Austria revisited Payr's work on haemangioma treatment [3,7,44]. To investigate the safety of Mg implants, they implanted subcutaneously and intramuscularly pure Mg wires (99.8%) with a diameter of 0.25 and 0.50 mm in rats and rabbits [44].…”

Section: Nerve and Muscle Tissuementioning

confidence: 99%