Resorption of Bone

Loutit, J. F.; Nisbet, N. W.

doi:10.1016/s0140-6736(79)90186-7

Cited by 55 publications

(9 citation statements)

References 17 publications

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“…This causes continuous increase of metaphyseal bone, giving with time an appearance of osteopetrosis ( Figure 1). A further morphologic resemblance between the bone of bisphosphonate treated rats and the bones in humans, and some forms of murine osteopetrosis (19,20), is that osteoclasts remain present even though they cease to function. Ineffective proliferation of osteoclasts seems to occur because despite loss of function, their number as well as the number of nuclei (1 3,2 1,22) is increased, and the metaphyseal bone has an increased content of lysosomal enzymes (13,23).…”

Section: Animal Pharmacologymentioning

confidence: 99%

“…A perhaps too simple explanation is that bisphosphonates, by binding to hydroxyapatite and slowing its dissolution, have a stabilizing action on bone and so alter its local microenvironment (13,21). There is evidence that bone resorbing cells belong to the hematopoietic system (19). The movement of such cells toward bone may be controlled by a chemotactic gradient from bone margins (25).…”

Section: Animal Pharmacologymentioning

confidence: 99%

“…There is reason to construe an alternative hypothesis, starting from the morphologic similarity between bone in osteopetrosis and that found in rats treated with bisphosphonates. There is evidence to suggest that cells from the immune system are involved in osteoclastic resorption, in particular that osteoclasts interact with the mononuclear phagocyte system (MPS) (19,20,26,27). Further, a substance has been found (osteoclast activating factor, OAF), produced by human peripheral mononuclear phagocytes in response to mitogens, that stimulates bone resorption in vitro (28).…”

Section: Animal Pharmacologymentioning

confidence: 99%

See 2 more Smart Citations

Apd in paget's disease of bone role of the mononuclear phagocyte system?

Bijvoet

Frijlink

Jie

et al. 1980

Arthritis & Rheumatism

125

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Section: Animal Pharmacologymentioning

confidence: 99%

Section: Animal Pharmacologymentioning

confidence: 99%

Section: Animal Pharmacologymentioning

confidence: 99%

See 1 more Smart Citation

Apd in paget's disease of bone role of the mononuclear phagocyte system?

Bijvoet

Frijlink

Jie

et al. 1980

Arthritis & Rheumatism

125

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“…When it was established that osteoclasts took origin from haemopoietic tissue, the mononuclear phagocyte was the first candidate for precursor (see Loutit and Morris, 1979;Marks, 1983;. However, it has not been possible so far to induce mature mononuclear phagocytes to differentiate into osteoclasts (Schneider and Byrnes, 1983;Burger et al, 1984;Chambers and Horton, 19841, and although osteoclasts show some similarities with cells of the mononuclear phagocyte lineage, there are some notable differences (see .…”

mentioning

confidence: 99%

Human macrophage colony‐stimulating factor inhibits bone resorption by osteoclasts disaggregated from rat bone

Hattersley

Dorey

Horton

et al. 1988

Journal Cellular Physiology

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Colony stimulating factors (CSFs) regulate the survival, proliferation and differentiation of haemopoietic progenitor cells, as well as the functional activity of mature cells. Because the osteoclast is derived from haemopoietic tissue, and because osteoblastic cells produce CSFs, we tested the effects of several CSFs on bone resorption by osteoclasts disaggregated from neonatal rat long bone. We found that recombinant macrophage (M)-CSF was a potent inhibitor of bone resorption, causing significant inhibition at concentrations similar to those required to support the growth of macrophage colonies in agar. Unlike other inhibitors of osteoclastic resorption, M-CSF did not alter cytoplasmic motility in time-lapse recordings, suggesting that M-CSF may inhibit osteoclasts through a different transduction mechanism. None of the remaining cytokines tested (granulocyte-macrophage CSF, interleukin 3, interleukin 6, or interferon gamma) influenced bone resorption. M-CSF production may be a mechanism by which osteoblastic cells, which produce M-CSF, may regulate osteoclastic function. Alternatively, inhibition of osteoclastic resorption by a CSF that is responsible for amplification of the macrophage compartment may reflect a close lineage relationship between mononuclear phagocytes, in which M-CSF induces a diversion of lineage resources away from osteoclastic function.

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“…The osteoclast is thought to be derived from immature marrow cells of the monocyte/macrophage lineage (1)(2)(3). However, the osteoclast has been difficult to study directly because ofits relative inaccessibility.…”

mentioning

confidence: 99%

1,25-Dihydroxyvitamin D3 causes formation of multinucleated cells with several osteoclast characteristics in cultures of primate marrow.

Roodman¹,

Ibbotson²,

MacDonald³

et al. 1985

Proc. Natl. Acad. Sci. U.S.A.

254

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The osteoclast is thought to be derived from immature marrow cells of the monocyte/macrophage lineage (1-3). However, the osteoclast has been difficult to study directly because ofits relative inaccessibility. Therefore investigators have used other cells of the monocyte/macrophage series, such as peritoneal macrophages (4), alveolar macrophages (5), HL-60 cells (6), and U937 cells (7), as models for osteoclasts or their precursors. Recently, it has been shown that the most potent biologically active metabolite of vitamin D, la,25-dihydroxyvitamin D3, causes fusion of alveolar macrophages (5) and differentiation of mouse leukemic cells and HL-60 and U937 human leukemic cells in culture into macrophages (6)(7)(8). Since the normal osteoclast precursor is believed to be present in the marrow mononuclear cell population, we cultured normal primate marrow mononuclear cells with 1,25-dihydroxyvitamin D3. We utilized the long-term culture system of Testa et al. (9), which they and we have used to examine formation of multinucleated cells with biologic and morphologic characteristics of osteoclasts in cultures of normal feline marrow (9, 10). We found that in the presence of 1,25-dihydroxyvitamin D3, a subpopulation of primate marrow mononuclear cells formed multinucleated cells with some of the characteristics of osteoclasts. MATERIALS AND METHODSCollection and Processing of Baboon Marrow Cells. Bone marrow was aspirated from the sternum or posterior iliac crest of adult baboons anesthetized with ketamine (10 mg/kg). Marrow was collected in syringes containing minimal essential medium alpha (a-MEM, GIBCO) supplemented with 5% (vol/vol) fetal bovine serum (Sterile Systems, Logan, UT) and preservative-free heparin (100 units/ml; Sigma). Marrow mononuclear cells were harvested from the interface of Ficoll/Hypaque density gradients (11) that had been centrifuged at 400 x g for 30 min at 12TC. The marrow mononuclear cells were washed twice with medium and then cultured in 24-well plates (Linbro) at 106 cells per ml, in a-MEM with 20o horse serum. In some experiments marrow mononuclear cells were incubated for 1 hr in a-MEM containing 20% fetal calf serum in 35-mm plastic tissue culture dishes, and the nonadherent cells were collected. The nonadherent cells were cultured in a similar fashion as the unfractionated marrow mononuclear cells. All cultures were maintained in a humidified 4% CO2 atmosphere at 370C. In selected experiments, various concentrations of osteotropic hormones were added at the initiation ofthe cultures and with each feeding. Cultures were fed weekly by removing half of the medium and nonadherent cells and replacing it with an equal volume of fresh medium. No attempt was made to recover the nonadherent cells. After culture, cells were fixed with 5% (vol/vol) glutaraldehyde (Sigma) and then stained with Wright stain. Cells were examined with an inverted phase-contrast microscope; those cells containing more than three nuclei were counted as multinucleated.Assay of Acid Phosphatase Activity. Marrow cells w...

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Resorption of Bone

Cited by 55 publications

References 17 publications

Apd in paget's disease of bone role of the mononuclear phagocyte system?

Apd in paget's disease of bone role of the mononuclear phagocyte system?

Human macrophage colony‐stimulating factor inhibits bone resorption by osteoclasts disaggregated from rat bone

1,25-Dihydroxyvitamin D3 causes formation of multinucleated cells with several osteoclast characteristics in cultures of primate marrow.

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