Resorption of bone by inflammatory cells derived from the joint capsule of hip arthroplasties

Athanasou, N A; Quinn, J; Bulstrode, C.

doi:10.1302/0301-620x.74b1.1732267

Cited by 141 publications

(64 citation statements)

References 13 publications

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“…A previous in vitro study showed resorption pits on bone slices incubated with inflammatory cells exposed to particles for 4 days [3]. We performed pre-and postexperimental microCT scans in vivo to detect changes in BMD.…”

Section: Methodsmentioning

confidence: 99%

“…Macrophages can resorb bone directly or differentiate into osteoclasts, the primary bone-resorbing cells, to undermine the prosthetic bed [3,33]; furthermore, the increased levels of reactive oxygen species produced during phagocytosis of wear debris will impair periprosthetic bone formation as a result of the increased cytotoxicity to osteoprogenitor cells [46]. Numerous in vivo and in vitro models have been developed to investigate the interactions among macrophages and other cells, wear debris, and osteolysis [1, 3, 8, 12, 16-20, 22, 29, 30, 33, 34, 42].…”

Section: Introductionmentioning

confidence: 99%

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Continuous Infusion of UHMWPE Particles Induces Increased Bone Macrophages and Osteolysis

Ren

Irani

Huang

et al. 2011

Clinical Orthopaedics &Amp; Related Research

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Background Aseptic loosening and periprosthetic osteolysis resulting from wear debris are major complications of total joint arthroplasty. Monocyte/macrophages are the key cells related to osteolysis at the bone-implant interface of joint arthroplasties. Whether the monocyte/macrophages found at the implant interface in the presence of polyethylene particles are locally or systemically derived is unknown. Questions/purposes We therefore asked (1) whether macrophages associated with polyethylene particle-induced chronic inflammation are recruited locally or systemically and (2) whether the recruited macrophages are associated with enhanced osteolysis locally.Methods Noninvasive in vivo imaging techniques (bioluminescence and microCT) were used to investigate initial macrophage migration systemically from a remote injection site to polyethylene wear particles continuously infused into the femoral canal. We used histologic and immunohistologic staining to confirm localization of migrated macrophages to the polyethylene particle-treated femoral canals and monitor cellular markers of bone remodeling.Results The values for bioluminescence were increased for animals receiving UHMWPE particles compared with the group in which the carrier saline was infused. At Day 8, the ratio of bioluminescence (operated femur divided by nonoperated contralateral femur of each animal) for the UHMWPE group was 13.95 ± 5.65, whereas the ratio for the saline group was 2.60 ± 1.14. Immunohistologic analysis demonstrated the presence of reporter macrophages in the UHMWPE particle-implanted femora only. MicroCT scans showed the bone mineral density for the group with both UHMWPE particles and macrophage was lower than the control groups. Conclusions Infusion of clinically relevant polyethylene particles, similar to the human scenario, stimulated systemic migration of remotely injected macrophages and local net bone resorption.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Continuous Infusion of UHMWPE Particles Induces Increased Bone Macrophages and Osteolysis

Ren

Irani

Huang

et al. 2011

Clinical Orthopaedics &Amp; Related Research

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“…However, attributable in part to PEEK's relatively inert and hydrophobic surface, recent evidence has demonstrated that smooth PEEK can exhibit poor osseointegration [9,25] and fibrous capsule formation around the implant [23,34]. Lack of bone-implant contact can induce micromotion and inflammation that leads to fibrous layer thickening, osteolysis, and implant loosening [2,13,29,37,48]. Previous studies [1,4,15,16,18,36] have shown that surface modifications such as plasma treatments, coatings, and composites can improve PEEK implant integration, yet many suffer practical limitations such as delamination, instability, and mechanical property tradeoffs.…”

Section: Introductionmentioning

confidence: 99%

“…(2) What is the effect of pore size on the mechanical properties of PEEK-SP? (3) Do surface porosity and pore size influence the cellular response to PEEK?…”

Section: Introductionmentioning

confidence: 99%

Do Surface Porosity and Pore Size Influence Mechanical Properties and Cellular Response to PEEK?

Torstrick

Evans

Stevens

et al. 2016

Clinical Orthopaedics &Amp; Related Research

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Background Despite its widespread use in orthopaedic implants such as soft tissue fasteners and spinal intervertebral implants, polyetheretherketone (PEEK) often suffers from poor osseointegration. Introducing porosity can overcome this limitation by encouraging bone ingrowth; however, the corresponding decrease in implant strength can potentially reduce the implant's ability to bear physiologic loads. We have previously shown, using a single pore size, that limiting porosity to the surface of PEEK implants preserves strength while supporting in vivo osseointegration. However, additional work is needed to investigate the effect of pore size on both the mechanical properties and cellular response to PEEK. Questions/purposes (1) Can surface porous PEEK (PEEK-SP) microstructure be reliably controlled? (2) What is the effect of pore size on the mechanical properties of PEEK-SP? (3) Do surface porosity and pore size influence the cellular response to PEEK? Methods PEEK-SP was created by extruding PEEK through NaCl crystals of three controlled ranges: 200 to 312, 312 to 425, and 425 to 508 lm. Micro-CT was used to characterize the microstructure of PEEK-SP. Tensile, fatigue, and interfacial shear tests were performed to compare the mechanical properties of PEEK-SP with injectionmolded PEEK (PEEK-IM). The cellular response to PEEK-SP, assessed by proliferation, alkaline phosphatase activity, vascular endothelial growth factor production, and calcium content of osteoblast, mesenchymal stem cell, and preosteoblast (MC3T3-E1) cultures, was compared with that of machined smooth PEEK and Ti6Al4V. Results Micro-CT analysis showed that PEEK-SP layers possessed pores that were 284 ± 35 lm, 341 ± 49 lm, and 416 ± 54 lm for each pore size group. Porosity and

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“…This is largely because direct evidence of bone resorption by these cells has not clearly been demonstrated. However, there is now increasing evidence to show that mononuclear phagocytes derived from inflammatory and neoplastic lesions are capable of bone resorption (Athanasou et al, 1989;Athanasou et al, 1991;Athanasou et al, 1992), and that osteoclast-like cells can form directly from tissue macrophages (Udagawa et al, 1990). …”

mentioning

confidence: 99%

Human tumour-associated macrophages are capable of bone resorption

Athanasou

Quinn²

1992

Br J Cancer

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Summary Cellular mechanisms of bone resorption associated with skeletal metastasis are poorly understood. Human tumour-associated macrophages (TAMs) isolated from primary lung carcinomas were incubated on bone slices where they formed resorption lacunae after 14 days co-culture with a mouse marrow-derived stromal cell line (ST2) with added la, 25-dihydroxy Vitamin D3 and dexamethasone. These co-cultures were associated with the formation of increased numbers of tartrate resistant acid phosphatase positive mononuclear and multinucleated cells. (Galasko, 1976), but the contribution of other cells, principally tumour cells and tumour-associated macrophages (TAMs) within the tumour deposits in bone, is uncertain (Galasko, 1976;Galasko, 1982;Eilon & Mundy, 1978; Koeffier et al., 1978;Novak et al., 1984). This is largely because direct evidence of bone resorption by these cells has not clearly been demonstrated. However, there is now increasing evidence to show that mononuclear phagocytes derived from inflammatory and neoplastic lesions are capable of bone resorption (Athanasou et al., 1989;Athanasou et al., 1991;Athanasou et al., 1992), and that osteoclast-like cells can form directly from tissue macrophages (Udagawa et al., 1990). Materials and methodsTAMs were isolated from five pneumoonectomy specimens for lung carcinoma (three adenocarcinomas; two squamous carcinomas) by collagenase digestion and substrate adherence from 2 x 2 x 2 cm portion of tumour (McGee & Myrvik, 1981), and alveolar macrophages (AMs) from equal portions of lung uninvolved by tumour (Haskill, 1981). TAM or AM-containing cell suspensions in alpha minimal essential medium plus 10% foetal calf serum (Gibco) (MEM/FCS) were added to 6mm wells (500cells/well) containing either bone slices or glass coverslips, half of which had been seeded (1,000 cells/well) 24 h earlier with the mouse marrow stromal cell line ST2 (Riken cell bank, Japan) (Athanasou et al., 1989). Four bone slices (half previously seeded with ST2 cells) were studied for each variable, and cell cultures were incubated at 37°C (5% CO2). After settling for 1 h, these were washed vigorously in MEM/FCS and placed in 16 mm wells containing aMEM/FCS. Dexamethasone (Sigma, UK) (10-7 M) and 1,25 dihydroxy Vitamin D3 (Roche, UK) (10-8 M) were added at the beginning of co-cultures with ST2 cells and at each change of medium (every 3 days). Adherent cells on bone slices were cultured for 24 h, 3, 7 and 14 days after which evidence of bone resorption was sought by scanning electron microscopy (SEM) (Chambers et al., 1984). The effect of PTH (NIBSC, UK) (10 IU ml), PGE2 (Sigma, UK) (10-5 M) and conditioned medium (derived from culture of ST2 cells alone) on bone resorption by TAMs and AMs isolated from two of the above specimens was also examined. Cells cultured on glass coverslips for similar periods were assessed for several osteoclast characteristics viz. morphological response to salmon calcitonin (Rorer Pharmaceuticals, UK) 1 tg ml-' (Chambers & Magnus, 1982), acid phosphatase (AP) and tart...

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Resorption of bone by inflammatory cells derived from the joint capsule of hip arthroplasties

Cited by 141 publications

References 13 publications

Continuous Infusion of UHMWPE Particles Induces Increased Bone Macrophages and Osteolysis

Continuous Infusion of UHMWPE Particles Induces Increased Bone Macrophages and Osteolysis

Do Surface Porosity and Pore Size Influence Mechanical Properties and Cellular Response to PEEK?

Human tumour-associated macrophages are capable of bone resorption

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