Resorcinol monophosphate ester as a new substrate for fluorometric detection of alkaline phosphatase activity in milk products

Capsulation of EBTAC into ZIF-8 for the development of a signal-on fluorescent biosensor to detect alkaline phosphatase

“…1–3 As a key diagnostic biomarker, its abnormally high level usually means the occurrence of a certain disease, such as liver cancer, osteocarcinoma, leukemia, jaundice, skeletal illness and hypothyroidism. 4–7 Therefore, the development of a simple and convenient assay for the determination of ALP is urgent. Up to now, many analytical assays for ALP have been designed based on colorimetric, 8,9 electrochemical, 10–13 ICP-MS 14 and surface-enhanced Raman scattering techniques.…”

“…To fabricate an ultrasensitive fluorescence biosensor, one of the key factors lies in discovering highly efficient and robust materials as the fluorescence probe. Traditionally, semiconductor quantum dots, 4,21 carbon nanomaterials, 22 noble metal nanomaterials 23 and organic conjugated polymers 24 are employed as the luminescent materials to fabricate the fluorescence biosensor for detecting ALP activity. However, some unavoidable objective factors will affect the fluorescence properties of the luminescent materials, and then interfere with the detection accuracy of the biosensor.…”