Resorbable Mg2+-Containing Phosphates for Bone Tissue Repair

Kazakova, G. K.; Сафронова, Т. В.; Golubchikov, Daniil; Shevtsova, Olga; Rau, Julietta V.

doi:10.3390/ma14174857

Cited by 42 publications

(33 citation statements)

References 132 publications

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“…In conclusion, the material described in this work successfully overcomes the main limitations of conventional MPCs, i.e., lack of macroporosity and poor drug release properties [15], by including templating ALN-loaded gelatin microparticles in the cement matrix.…”

Section: Discussionmentioning

confidence: 88%

“…Despite the great number of appealing features of phosphate-based cements for biomedical applications, one of the major limitations of both CPCs and MPCs is their lack of macroporosity [6,[14][15][16]. While such cements display intrinsic microporosities, which allow for protein adsorption, cell attachment and permeability of the material to body fluids, they typically lack of interconnected macropores over 100 lm which would allow for angiogenesis and bone ingrowth, promoting the fast resorption of the cement [17].…”

Section: Graphical Abstract Introductionmentioning

confidence: 99%

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Alendronate-loaded gelatin microparticles as templating agents for macroporous magnesium phosphate-based bone cements

et al. 2022

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Magnesium phosphate-based cements (MPCs) have recently attracted great attention as materials for bone repair. However, the lack of macroporosity, fundamental for cells permeation and bone ingrowth, is one of the main limitations hampering MPCs full exploitation. In this work gelatin microparticles are exploited as templating agents for the creation of macroporosities in MPCs. In addition, gelatin particles were loaded with a well-recognized drug for the treatment of osteoporosis, alendronate, to locally release the therapeutic agent. Gelatin microparticles of different size were prepared with a simple water-in-oil emulsion method and included in MPCs at various concentrations. The properties of both the MPCs and the final material were characterized by assessing the composite in terms of injectability, setting time, infrared spectroscopy, scanning electron microscopy and confocal Raman microscopy. The MPC-gelatin composites were then incubated in water at physiological temperature, to promote the dissolution of the gelatin, obtain a macroporous cement, and release gelatin and alendronate. The obtained results show that gelatin microparticles have a twofold action as they allow for the formation of MPC with an interconnected and hundreds of µm-sized porosity and the local release of alendronate, resulting in a material with ideal features for bone repair. Graphical abstract

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Section: Discussionmentioning

confidence: 88%

Section: Graphical Abstract Introductionmentioning

confidence: 99%

Alendronate-loaded gelatin microparticles as templating agents for macroporous magnesium phosphate-based bone cements

et al. 2022

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“…Although various mechanisms by which Mg is absorbed/incorporated during hydroxyapatite crystallization have already been studied [12], the influence of this dopant on the EPR signal of substituted hydroxyapatite is still not thoroughly understood, particularly at temperatures above room temperature. Moreover, the exact location of the CO − 2 and CO − 3 radicals in biological and synthetic apatites is still under debate.…”

Section: Thermal Behaviour-accelerated Ageingmentioning

confidence: 99%

“…In biological apatites, Ca + ions can be substituted with Na + , K + , Mg 2+ , Sr 2+ , PO 3− 4 or HPO 2− 4 ions with CO 2− 3 and OH − ions can be substituted with F − , Cl − , CO 2− 3 [6]. Among the different possible substitute ions, Mg 2+ attracts special attention as it is an important trace element in bone and teeth [1,11,12]. It plays a key role in bone metabolism since it influences osteoblast and osteoclast activity, and thereby bone growth.…”

Section: Introductionmentioning

confidence: 99%

A Comparative EPR Study of Non-Substituted and Mg-Substituted Hydroxyapatite Behaviour in Model Media and during Accelerated Ageing

et al. 2022

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To assess the application potential of novel biomaterials, their behaviour in model media and upon sterilization should be investigated, as well as the stability related to their storage conditions. Such data are lacking for Mg-substituted HAP (Mg-HAP). Therefore, the changes in the local structure of non-substituted and Mg-HAP after irradiation and immersion in corrected simulated fluid and saline solution for 28 days were followed by electron paramagnetic resonance (EPR) spectroscopy for the first time. To better understand the stability of radical species induced by sterilization, EPR spectra of samples kept for 2 h at temperatures up to 373 K were recorded to provide an insight into the stability of the sample storage conditions by the accelerated aging method. Samples were characterized by PXRD, FTIR, SEM, EDS, AAS and TGA. Results confirmed that irradiation does not induce changes in the composition or the structure of any of the investigated materials. Fading or the complete disappearance of radical signals in the EPR spectra after immersion in both media was accompanied by the disappearance of other phases formed as a minor byproduct in the synthesis of substituted HAP, as confirmed by PXRD and FTIR analysis. Obtained results confirm the great potential of Mg-HAPs for biomedical applications, although closer attention should be given to the processes related to sample storage stability at different temperatures.

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“…It is also essential for the DNA and RNA synthesis processes and has been found to be significantly reduced in the serum of RA patients [ 15 ]. For all these reasons, these cations, among others, have been extensively incorporated into multiple biomaterials envisioned for regenerative applications [ 16 , 17 , 18 ]. In this regard, our research group has prepared metallic complexes of folic acid with Ca, Sr, Zn, Mg, and Mn, and studied them in the context of musculoskeletal regeneration.…”

Section: Introductionmentioning

confidence: 99%

Development of Methotrexate Complexes Endowed with New Biological Properties Envisioned for Musculoskeletal Regeneration in Rheumatoid Arthritis Environments

Fernández‐Villa

Ramírez-Jiménez

Aranaz

et al. 2022

IJMS

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Methotrexate (MTX) administration is the gold standard treatment for rheumatoid arthritis (RA), but its effects are limited to preventing the progression of the disease. Therefore, effective regenerative therapies for damaged tissues are still to be developed. In this regard, MTX complexes of general molecular formula M(MTX)·xH2O, where M = Sr, Zn, or Mg, were synthesized and physicochemically characterized by TGA, XRD, NMR, ATR–FTIR, and EDAX spectroscopies. Characterization results demonstrated the coordination between the different cations and MTX via two monodentate bonds with the carboxylate groups of MTX. Cation complexation provided MTX with new bioactive properties such as increasing the deposition of glycosaminoglycans (GAGs) and alternative anti-inflammatory capacities, without compromising the immunosuppressant properties of MTX on macrophages. Lastly, these new complexes were loaded into spray-dried chitosan microparticles as a proof of concept that they can be encapsulated and further delivered in situ in RA-affected joints, envisioning them as a suitable alternative to oral MTX therapy.

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Resorbable Mg2+-Containing Phosphates for Bone Tissue Repair

Cited by 42 publications

References 132 publications

Alendronate-loaded gelatin microparticles as templating agents for macroporous magnesium phosphate-based bone cements

Alendronate-loaded gelatin microparticles as templating agents for macroporous magnesium phosphate-based bone cements

A Comparative EPR Study of Non-Substituted and Mg-Substituted Hydroxyapatite Behaviour in Model Media and during Accelerated Ageing

Development of Methotrexate Complexes Endowed with New Biological Properties Envisioned for Musculoskeletal Regeneration in Rheumatoid Arthritis Environments

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