Resonator design for in vivo ESR spectroscopy

Sotgiu, A.

doi:10.1016/0022-2364(85)90002-2

Cited by 29 publications

(26 citation statements)

References 8 publications

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“…While first demonstrated to show significantly improved sensitivity at X-band, constructing the lower frequency L-band analog was straightforward; in fact, the tolerances were more forgiving than for X-band. Subsequently the loop-gap resonator and a closely related reentrant cavity developed by Sotgiu and colleagues [83] became quite common to in-vivo EPR instrumentation. The L-band reentrant cavity resonator, which was quite suitable for whole body experiments on mice, was developed at the University of L'Aquila, Italy and then later at the Milwaukee EPR Center [38,83].…”

Section: L-band In-vivo Eprmentioning

confidence: 99%

“…Subsequently the loop-gap resonator and a closely related reentrant cavity developed by Sotgiu and colleagues [83] became quite common to in-vivo EPR instrumentation. The L-band reentrant cavity resonator, which was quite suitable for whole body experiments on mice, was developed at the University of L'Aquila, Italy and then later at the Milwaukee EPR Center [38,83]. We invited Antonello Sotgiu from L'Aquila to our laboratory where he developed a reentrant resonator for our pharmacokinetic studies on the rates of biodestruction/elimination of several aminoxyl/nitroxide probes in mice and rats [15].…”

Section: L-band In-vivo Eprmentioning

confidence: 99%

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The evolution of biomedical EPR (ESR)

Berliner

2017

BSI

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Abstract. Since the first EPR/ESR spectrum of a paramagnetic substance was published over 70 years ago, the technical improvements did not occur until after/during World War II with the advent of radar technology. The approaches to biomedical problems started somewhat later with the real burst of activity starting after the birth of the spin label technique about 50 years ago. The applications to proteins, then membranes and nucleic acids, and later applications to cells and eventually in-vivo on small animals and now humans has led EPR/ESR to finally being recognized as a uniquely powerful technique in the toolbox of techniques probing macromolecules and their interactions, free radical biology and its eventual value as a diagnostic technique. This article gives an overview of EPR/ESR studies of biomedically related systems, including proteins and enzymes. It presents a very personal historical perspective, briefly reviews the origins of the technique and reflects on possible future directions. As with NMR, advances in molecular biology and technology drastically changed the nature and focus of the technique, particularly the site directed spin labeling method that has been invaluable in determining protein and macromolecular structure by both EPR and NMR.

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Section: L-band In-vivo Eprmentioning

confidence: 99%

Section: L-band In-vivo Eprmentioning

confidence: 99%

The evolution of biomedical EPR (ESR)

Berliner

2017

BSI

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“…Re-entrant resonators (RER) do not require a shield; however, since they were constructed from milled and silver plated plastic they had low rigidity and consequently microphonics, and poor thermal stability. 68 Ceramics, being a rigid material with high structural strength and stable mechanical and thermal parameters, are thus a good choice for resonator construction. Several RER sample resonators have been designed and fabricated using ceramics.…”

Section: Resonators For Biological Samplesmentioning

confidence: 99%

Cardiac applications of EPR imaging

Kuppusamy

Zweíer

2004

NMR in Biomedicine

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This review summarizes the development and application of a variety of EPR imaging modalities including spatial, spectral-spatial (spectroscopic), gated-imaging and oxygen mapping to cardiovascular studies. It has been hypothesized that free radical metabolism, oxygenation and nitric oxide generation in biological organs such as the heart may vary over the spatially defined tissue structure. We have developed instrumentation optimized for 3D spatial and 3D or 4D spectral-spatial imaging of free radicals at 1.2 GHz. Using this instrumentation high quality 3D spectral-spatial imaging of nitroxyl (nitroxide) metabolism was performed, as well as spatially localized measurements of oxygen concentrations, based on the oxygen-dependent line-broadening of the EPR spectrum. Both exogenously infused probes and endogenous radicals were used to obtain the images. It is demonstrated that the EPR imaging is a powerful tool which can provide unique information regarding the spatial localization of free radicals, oxygen and nitric oxide in biological organs and tissues.

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“…The development of low frequency EPR instrumentation at L-band, 1-2 GHz, or lower frequencies, and lumped circuit resonators has made it possible to perform EPR measurements on these lossy samples (6)(7)(8)(9)(10)(11)(12). Previous in vivo or ex vivo EPR spectroscopy studies have focused on global measurements of free radical metabolism and measurements of tissue oxygenation (9)(10)(12)(13)(14)(15).…”

Section: Introductionmentioning

confidence: 99%

Three‐dimensional spatial EPR imaging of the rat heart

Kuppusamy

Wang

Zweíer

1995

Magnetic Resonance in Med

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While instrumentation capable of performing three-dimensional EPR imaging of free radicals in whole tissues and isolated organs has been developed at L-band, important questions remain regarding the resolution and image quality that can be obtained in practice using the presently available free radical labels. Therefore, studies were performed applying three-dimensional spatial EPR imaging at L-band to image the distribution of free radical labels in the isolated heart and in phantoms of similar size. With nitroxide labels the obtainable resolution is limited by the presence of hyperfine structure in the EPR absorption function that in turn limits the maximum applicable gradient. The authors observed that with the nitroxide labels, resolutions in the range of 1-2 mm are possible, while with a single line glucose char label, resolutions of 0.2 mm are obtained. With the nitroxides, images were of sufficient resolution to resolve the overall global shape of the heart and the location of the left and right ventricular cavities; however, finer structures could not be resolved. With the glucose char much finer resolution could be obtained enabling visualization of the ventricles, aortic root, and proximal coronary arteries.

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Resonator design for in vivo ESR spectroscopy

Cited by 29 publications

References 8 publications

The evolution of biomedical EPR (ESR)

The evolution of biomedical EPR (ESR)

Cardiac applications of EPR imaging

Three‐dimensional spatial EPR imaging of the rat heart

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