Using TRIUMFs off-line laser ion source test stand with a system of tunable titanium sapphire lasers, the polarization dependence of laser resonance ionization has been investigated using beryllium. A significant polarization dependence was observed for the excitation pathwhich are typical transitions for alkaline and alkaline-like elements. This polarization dependence was further verified on Be radioactive isotopes at TRIUMF's isotope separator and accelerator facility (ISAC). Laser polarization was proven to be an important parameter in operating resonance ionization laser ion sources (RILIS).The polarization spectroscopy was preformed off-line both on the 2p 2 1 S 0 autoionizing (AI) state and high-n Rydberg states of the 2sns 1 S 0 and 2snd 1 D 2 series. The energy of the 2p 2 1 S 0 AI state and ionization potential (IP) of beryllium were extracted as 76167(6) cm −1 and 75192.59(3) cm −1 .Polarization spectroscopy can be used to determine the J values of newly found states in in-source spectroscopy of the complex/radioactive alkaline-like elements such as Ra, Sm, Yb, Pu and No.