Resonance Rayleigh-scattering method for the determination of proteins with gold nanoparticle probe

Liu, Shaopu; Yang, Zhuo; Liu, Zhongfang; Kong, Lingqian

doi:10.1016/j.ab.2006.03.012

Cited by 81 publications

(24 citation statements)

References 33 publications

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“…Moreover, the decrease in the amount of protein differed when varying the TMX concentrations at all pH. The phenomenon further proved that TMX could induce protein aggregation and even lead to precipitation at pH = 5.4 [66].…”

Section: Resonance Light Scattering Spectra Of the Systemmentioning

confidence: 84%

Investigation on the interaction between tamoxifen and human holo-transferrin: Determination of the binding mechanism by fluorescence quenching, resonance light scattering and circular dichroism methods

Sarzehi¹,

Chamani²

2010

International Journal of Biological Macromolecules

154

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Section: Resonance Light Scattering Spectra Of the Systemmentioning

confidence: 84%

Investigation on the interaction between tamoxifen and human holo-transferrin: Determination of the binding mechanism by fluorescence quenching, resonance light scattering and circular dichroism methods

Sarzehi¹,

Chamani²

2010

International Journal of Biological Macromolecules

154

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“…Furthermore, the increase of the molecular volume and the enhancement of surface hydrophobicity (neutralization of molecular electricity and lots of hydrophobic aryls and alkyl frameworks in the molecule), hydrophobic interface between the ion-association complex and aqueous phase, and result in a surface-enhanced Rayleigh scattering effect. All of the above may result in the enhancement of resonance Rayleigh scattering [29][30][31] .…”

Section: Reaction Mechanismmentioning

confidence: 96%

Study on the interaction between diphenhydramine and erythrosin by absorption, fluorescence and resonance Rayleigh scattering spectra

et al. 2007

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In pH 4.5 Britton-Robinson (BR) buffer solution, erythrosin (ET)can react with diphenhydramine (DP) to form a 1:1 ion-association complex, which not only results in the change of the absorption spectra, but also results in the great enhancement of resonance Rayleigh scattering (RRS) and the quenching of fluorescence. Furthermore, a new RRS spectrum will appear, and the maximum RRS wavelength was located at about 580 nm．In this work, the spectral characteristics of the absorption, fluorescence and RRS, the optimum conditions of the reaction and the properties of an analytical chemistry were investigated. A sensitive, simple and new method for the determination of DP by using erythrosin as a probe has been developed. The detection limits for DP were 0.0020 μg/mL for RRS method, 0.088 μg/mL for absorption method and 0.094 μg/mL for fluorophotometry. There was a linear relationship between the absorbance, RRS and fluorescence intensities and the drug concentration in the range of 0.0067-2.0, 0.29-6.4 and 0.31-3.2 μg/mL, respectively. The effects of the interaction of diphenhydramine and erythrosin on the absorption, fluorescence and resonance Rayleigh scattering spectra were discussed. In light polarization experiment, the polarization of RRS at maximum wavelength was measured to be P = 0.9779, and it revealed that the RRS spectrum of DP-ET complex consists mostly of resonance scattering and few resonance fluorescence. In this study, enthalpy of formation and mean polarizability were calculated by AM1 quantum chemistry method. In addition, the reaction mechanism and the reasons for the enhancement of scattering spectra and the energy transfer between absorption, fluorescence and RRS were discussed.resonance Rayleigh scattering, spectrophotometry, fluorescence quenching method, diphenhydramine, erythrosin Diphenhydramine (DP) is an antihistamine. It blocks the effects of the naturally occurring chemical histamine in the body. It is used to treat all kinds of symptoms of allergies and suppress coughs and to treat mild forms of Parkinson's disease. However, DP and its metabolites have a series of side effects, such as dizziness, headache, extreme sleepiness, even eclampsia, exanimation, cardiac inhibition, respiratory paralysis. In combination with other drugs, it may induce hypertension and arrhythmia [1,2] . In order to get better therapeutic effect and less side effects, it is very necessary for pharmaceutical analysis and clinical medicine to determine diphenhydramine quantitatively. At present, only high concentration of DP can be determined by titration method [3] because of its low sensitivity and difficulty for judging the end point. Many analytical techniques including spectrophotometry (SP) [4,5] , spectrofluorometry (F) [6,7] , highperformance liquid chromatography (HPLC) [8,9] , gas chromatography (GC) [10] , liquid chromatography-mass spectrometry (LC-MS) [11] , capillary electrophoresis

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“…Resonance Rayleigh scattering (RRS), owing to its high sensitivity, simplicity, and rapidness in performance, has been applied to many fields, such as biological macromolecules (Luo et al 2001;Liu et al 2006;Itoh et al 2006;Cai et al 2009;Bi et al 2012), pharmaceuticals (Fu et al 2007;Chen et al 2009a, b;Qin et al 2009), environment (Cui et al 2007(Cui et al , 2008Liu et al 2000Liu et al , 2011Yang et al 2009;Xiao et al 2008), natural products (Xiang et al 2011), and some physicochemical parameters (Chen et al 2009a, b;Li et al 2005;Shi et al 2011). Nowadays, the RRS technique is gradually being used to study system reaction mechanisms Huang et al 2012).…”

Section: Introductionmentioning

confidence: 98%

A Highly Sensitive Resonance Rayleigh Scattering Method for the Determination of Vitamin C Based on the Formation of Zirconium Hexacyanoferrate (II) Nanoparticles

2015

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In pH 2.55 HCl solution, vitamin C (VC) could deoxidize [Fe(CN) 6 ] 3− producing [Fe(CN) 6 ] 4− , which reacted with Zr (IV) to form Zr[Fe(CN) 6 ] complex, then aggregated to produce {Zr[Fe(CN)6]} n nanoparticles by virtue of hydrophobic force and van der Waals force. The intensity of RRS at 340 nm is directly proportional to the concentration of VC in the range of 0.1-1.45 μg mL −1 , and the detection limit (3 s/k) is 0.128 ng mL −1 . In this work, the spectral characteristics of RRS, the optimum condition, the reaction mechanism of the reaction, and their influencing factors especially the effect of organic solvent have been studied. The sensitive, rapid, and simple method based on resonance Rayleigh scattering was successfully applied to determine trace amount VC in VC injection and tablet.

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Resonance Rayleigh-scattering method for the determination of proteins with gold nanoparticle probe

Cited by 81 publications

References 33 publications

Investigation on the interaction between tamoxifen and human holo-transferrin: Determination of the binding mechanism by fluorescence quenching, resonance light scattering and circular dichroism methods

Investigation on the interaction between tamoxifen and human holo-transferrin: Determination of the binding mechanism by fluorescence quenching, resonance light scattering and circular dichroism methods

Study on the interaction between diphenhydramine and erythrosin by absorption, fluorescence and resonance Rayleigh scattering spectra

A Highly Sensitive Resonance Rayleigh Scattering Method for the Determination of Vitamin C Based on the Formation of Zirconium Hexacyanoferrate (II) Nanoparticles

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