Resonance Rayleigh scattering approach based on association complex formation with erythrosine B for determination of venlafaxine, application to the dosage form and spiked human plasma

In this work, two validated approaches were used for estimating hydroxyzine HCl for the first time using resonance Rayleigh scattering (RRS) and spectrofluorimetric techniques. The suggested approaches relied on forming an association complex between hydroxyzine HCl and 2,4,5,7‐tetraiodofluorescein (erythrosin B) reagent in an acidic media. The quenching in the fluorescence intensity of 2,4,5,7‐tetraiodofluorescein by hydroxyzine at 551.5 nm (excitation = 527.5 nm) was used for determining the studied drug by the spectrofluorimetric technique. The RRS approach is based on amplifying the RRS spectrum at 348 nm upon the interaction of hydroxyzine HCl with 2,4,5,7‐tetraiodofluorescein. The spectrofluorimetric methodology and the RRS methodology produced linear results within ranges of 0.15–1.5 μg ml−1 and 0.1–1.2 μg ml−1, respectively. LOD values for these methods were determined to be 0.047 μg ml−1 and 0.033 μg ml−1, respectively. The content of hydroxyzine HCl in its pharmaceutical tablet was estimated using the developed procedures with acceptable recoveries. Additionally, the application of four greenness and whiteness algorithms shows that they are superior to the previously reported method in terms of sustainability, economics, analytical performance, and practicality.

Section: Introductionmentioning

confidence: 99%

Design of resonance Rayleigh scattering and spectrofluorimetric methods for the determination of the antihistaminic drug, hydroxyzine, based on its interaction with 2,4,5,7‐tetraiodofluorescein: Evaluation of analytical eco‐scale and greenness/whiteness algorithms

Abdel‐Lateef,

Darwish,

Gomaa

et al. 2024

“…As a result, it has gained recognition as a promising tool for protein quantification. Moreover, it is used as a fluorescence probe for tracking many medicaments such as dapoxetine [21], duloxetine [22], venlafaxine [23], terbinafine [24], rupatadine [25], and cyclobenzaprine [26]. Here, the ideal use of pyrosin B (2‐[6‐hydroxy‐2,4,5,7‐tetraiodo‐3‐oxo‐xanthen‐9‐yl]benzoic acid) as fluorogenic strategy for feasible PCD assay.…”

Section: Introductionmentioning

confidence: 99%

An ingenious technique based on the usage of fluorone‐based dye; pyrosin B in prucalopride assay in different matrices through an “on–off” dye native fluorescence probe

Abu‐hassan,

Mahdi,

Alshehri

et al. 2024

Self Cite

Prucalopride (PCD), is a modern medication approved by the United States in 2018 to alleviate constipation caused by motility issues. PCD demonstrates a strong affinity and selectivity toward the 5‐HT4 receptor. The study here introduces a feasible, direct, non‐extractive, and affordable pathway for PCD analytical tracking. The fluorimetric study is based on the on–off effect on the emission amplitude of fluorone‐based dye (pyrosin B). In a one‐pot experiment, the complex between PCD and pyrosin B is formed instantly in an acidic medium. Correlation between decreased pyrosin B emission and PCD concentrations provides a linear calibration plot from 50 to 900 ng/mL. PCD–dye complex system affecting variables were meticulously tuned. The values of the estimated limit of quantitation and limit of detection for the current methodology were 47.5 and 15.7 ng/mL, respectively. Conformity of the strategy validity was achieved by a comprehensive study of the International Council for Harmonization of Technical Requirements for Pharmaceuticals for Human Use criteria. The method was convincingly applied for PCD assay in tablets and content uniformity investigation. Furthermore, PCD tracking in the spiked biological fluid was applied. Finally, the method uses distilled water as dispersing medium which rise accommodation with the green chemistry principle.

“…Hence, it has been recognized as a valuable tool for protein quantification. In addition, erythrosine B serves as a fluorescence probe for tracking various medications such as dapoxetine [21], daclatasvir [22, 23], duloxetine [24], venlafaxine [25], terbinafine [26], rupatadine [27], naftidrofuryl [28], and cyclobenzaprine [29]. This makes it an ideal fluorogenic strategy for a feasible assay of DLZ.…”

Section: Introductionmentioning

confidence: 99%

Utility of native emission quenching of erythrosine B for the determination of diltiazem in different dosage forms

Abdel‐Lateef,

Darling,

Darwish

2024

This study introduces a practical and cost‐effective method for tracking diltiazem (DLZ) analytically. It utilizes a fluorimetric approach that relies on the modulation of fluorescence intensity of a dye called erythrosine B. Through a one‐pot experiment performed in an acidic environment, a complex is rapidly formed between DLZ and erythrosine B. By observing the decrease in erythrosine B emission, a linear calibration plot is established, enabling the detection and quantification of DLZ concentrations ranging from 40 to 850 ng/ml. The estimated limits of detection and quantitation were 10.5 and 32.1 ng/ml, respectively. The variables affecting the DLZ‐dye complex system were carefully adjusted. The validity of the approach was confirmed through a thorough evaluation based on the criteria set by ICH guidelines. The accuracy and precision of the methodology were evaluated, and the standard deviation and relative standard deviation were below 2. The strategy was successfully employed to analyze DLZ in tablets and capsules, and no significant variation between the proposed and reported methods as the values of the estimated t‐test and F‐test at five determinations were below 2.306 and 6.338, respectively. Notably, the method adheres to the principle of green chemistry by utilizing distilled water as the dispersing medium.