Resonance light scattering determination of metallothioneins using levofloxacin−palladium complex as a light scattering probe

Determination of ATP by resonance light scattering using a binuclear uranyl complex and aptamer modified gold nanoparticles as optical probes

“…It has the advantages of simplicity, rapidness and high sensitivity. RLS method has been used for the determination of various target analytes, including inorganic ions [13,14], organic medicines [15][16][17] and biological macromolecules [18][19][20][21], as well as the interactions of medicines with biological macromolecules [22,23]. However, until now, RLS method has hardly been used for the determination of ATP.…”

Determination of trace metallothioneins at nanogram levels with Eosin Y by resonance light scattering method

“…Urine specimens were pre-treated as described in the literature, with appropriate modification [23,35]. After mixing completely, a 6.0 mL aliquot of the urine sample was mixed with a 6.0 mL of 0.01 mol L −1 Tris-HCl buffer solution (pH 8.6) with shaking, then centrifuged for 20 min at 4000 rpm at 4°C.…”

“…Furthermore, it can be accomplished with a common fluorescence spectrometer using inexpensive and safe reagents [32][33][34]. Wang's group [35,36] used levofloxacin-palladium, ciprofloxacin-Cu(II) complex as RLS probe and got reliable and sensitive results. These RLS methods could provide a promising potential for the detection of MTs.…”

Determination of Trace Metallothioneins at Nanomolar Levels Using Phenanthroline–Copper Coordination by Fluorescence Spectra

“…This experimental result indicated that MTs perhaps react with Phen-Cu 2+ to form the triple complex. 25,[27][28] There was no fluorescence of Cu 2+ , MTs alone in pH 7.0 B-R buffer. There was a strong fluorescent signal of Phen in pH 7.0 B-R buffer as shown in Fig.…”

“…Fish tissue samples were pretreated according to the literature with appropriate modifications. 25,28 A specified volume of tissue was extracted with an equal volume of 2.0 × 10 -2 mol L -1 pH 8.6 Tris-HCl buffer, followed by centrifugation (4 C, 4000 rpm, 20 min). The supernatant was heated at 80 C for 10 min, cooled to 4 C and centrifuged (4 C, 4000 rpm, 20 min).…”