Resonance assignment of PsbP: an extrinsic protein from photosystem II of Spinacia oleracea

Rathner, Adriana; Chandra, Kousik; Rathner, Petr; Horničáková, Michaela; Schlagnitweit, Judith; Kohoutová, Jaroslava; Ettrich, Rüdiger; Müller, Norbert

doi:10.1007/s12104-015-9606-2

Cited by 3 publications

(1 citation statement)

References 22 publications

(27 reference statements)

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“…They may very likely adopt different conformations and exhibit different dynamics during the assembly process of the OEC, regulation of ion concentration while anchored to the thylakoid, and when performing the aforementioned repair function, respectively. Based on the current results and the recent NMR assignment of PsbP, which both are probably most relevant for the assembly stage, our future investigations will include all extrinsic Psb proteins of the OEC found in higher plants. Exploring their mutual interactions as well as the influence of the ionic and membrane environment on their structural and dynamic features will reveal their functions in greater detail.…”

Section: Resultsmentioning

confidence: 88%

Solution NMR and molecular dynamics reveal a persistent alpha helix within the dynamic region of PsbQ from photosystem II of higher plants

Rathner

Horničáková

et al. 2015

Proteins

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The extrinsic proteins of photosystem II of higher plants and green algae PsbO, PsbP, PsbQ, and PsbR are essential for stable oxygen production in the oxygen evolving center. In the available X‐ray crystallographic structure of higher plant PsbQ residues S14‐Y33 are missing. Building on the backbone NMR assignment of PsbQ, which includes this “missing link”, we report the extended resonance assignment including side chain atoms. Based on nuclear Overhauser effect spectra a high resolution solution structure of PsbQ with a backbone RMSD of 0.81 Å was obtained from torsion angle dynamics. Within the N‐terminal residues 1–45 the solution structure deviates significantly from the X‐ray crystallographic one, while the four‐helix bundle core found previously is confirmed. A short α‐helix is observed in the solution structure at the location where a β‐strand had been proposed in the earlier crystallographic study. NMR relaxation data and unrestrained molecular dynamics simulations corroborate that the N‐terminal region behaves as a flexible tail with a persistent short local helical secondary structure, while no indications of forming a β‐strand are found. Proteins 2015; 83:1677–1686. © 2015 The Authors. Proteins: Structure, Function, and Bioinformatics Published by Wiley Periodicals, Inc.

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Section: Resultsmentioning

confidence: 88%

Solution NMR and molecular dynamics reveal a persistent alpha helix within the dynamic region of PsbQ from photosystem II of higher plants

Rathner

Horničáková

et al. 2015

Proteins

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The efficient capture of polysaccharides in Tetradesmus obliquus of indole-3-acetic acid coupling sludge extraction

Yu,

Ding,

et al. 2024

Science of The Total Environment

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Silver-, calcium-, and copper molybdate compounds: Preparation, antibacterial activity, and mechanisms

et al. 2017

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Developing novel compounds with antimicrobial properties can be an effective approach to decreasing the number of healthcare-associated infections, particularly in the context of medical devices and touch surfaces. A variety of molybdate powders (AgMoO, CaMoO, CuMoO and CuMoO) were synthesized and characterized, and Escherichia coli was used as a model gram-negative bacterium to demonstrate their antimicrobial properties. Optical density measurements, bacterial colony growth, and stained gel images for protein expression clearly showed that silver- and copper molybdates inhibit bacterial growth, whereas CaMoO exhibited no bactericidal effect. All tests were performed in both daylight and darkness to assess the possible contribution of a photocatalytic effect on the activity observed. The main mechanism responsible for the antibacterial effect observed for AgMoO is related to Ag release in combination with medium acidification, whereas for compounds containing copper, leaching of Cu ions is proposed. All these effects are known to cause damage at the cellular level. A photocatalytic contribution to the antibacterial activity was not clearly observable. Based on the pH and solubility measurements performed for powders in contact with various media (ultrapure water and bacterial growth medium), silver molybdate (AgMoO) was identified as the best antibacterial candidate. This compound has great potential for further use in hybrid powder-polymer/varnish systems for touch surfaces in healthcare settings.

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Resonance assignment of PsbP: an extrinsic protein from photosystem II of Spinacia oleracea

Cited by 3 publications

References 22 publications

Solution NMR and molecular dynamics reveal a persistent alpha helix within the dynamic region of PsbQ from photosystem II of higher plants

Solution NMR and molecular dynamics reveal a persistent alpha helix within the dynamic region of PsbQ from photosystem II of higher plants

The efficient capture of polysaccharides in Tetradesmus obliquus of indole-3-acetic acid coupling sludge extraction

Silver-, calcium-, and copper molybdate compounds: Preparation, antibacterial activity, and mechanisms

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