Resolving sorting mechanisms into exosomes

Stoorvogel, Willem

doi:10.1038/cr.2015.39

Cited by 71 publications

(76 citation statements)

References 11 publications

(15 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There are also ESCRT independent mechanisms of MVB, ILV, and exosome formation; these mechanisms can involve lipids such as ceramide and cholesterol, phospholipase D2, or tetraspanins [27,29]. Thinking of the early endosome as a signaling entity, it is easy to conceive how exosomal content can be determined by the membrane constituents of the early endosome.…”

Section: Sortingmentioning

confidence: 98%

“…The ESCRT machinery consists of four ESCRT protein complexes and associated proteins; ESCRT-0 functions in a ubiquitin-dependent fashion to cluster cargo, ESCRT-I and ESCRT-II are involved in budding, while ESCRT-III functions in vesicle scission [27,29]. Accessory proteins include ALIX and VPS4; ALIX is involved in vesicle budding and VPS4 assists with scission [27,29].…”

Section: Sortingmentioning

confidence: 99%

“…The early endosome recruits the ESCRT machinery for the formation of ILVs; the ILVs are pre-exosomes [27,29]. The ESCRT machinery consists of four ESCRT protein complexes and associated proteins; ESCRT-0 functions in a ubiquitin-dependent fashion to cluster cargo, ESCRT-I and ESCRT-II are involved in budding, while ESCRT-III functions in vesicle scission [27,29].…”

Section: Sortingmentioning

confidence: 99%

See 2 more Smart Citations

Exosome mediated communication within the tumor microenvironment

Milane

Singh

Mattheolabakis

et al. 2015

Journal of Controlled Release

587

466

View full text Add to dashboard Cite

Section: Sortingmentioning

confidence: 98%

Section: Sortingmentioning

confidence: 99%

Section: Sortingmentioning

confidence: 99%

See 1 more Smart Citation

Exosome mediated communication within the tumor microenvironment

Milane

Singh

Mattheolabakis

et al. 2015

Journal of Controlled Release

587

466

View full text Add to dashboard Cite

“…NSE is likely released from dying neurons as a marker of cell disintegration. In contrast, neural exosomes production may require largely intact intracellular mechanisms 34, 35,. although extracellular vesicles can also be produced during apoptosis via an alternative “beads on a string” mechanism 36.…”

Section: Discussionmentioning

confidence: 99%

Diagnostic Potential of Neural Exosome Cargo as Biomarkers for Acute Brain Injury

Goetzl

Merabova

Darbinian

et al. 2017

Ann Clin Transl Neurol

View full text Add to dashboard Cite

ObjectiveNeuronal exosomes purified from peripheral blood samples have been proposed as diagnostic tool in the setting of acute brain injury but never tested clinically. We hypothesized that exosome protein biomarkers would change over time following acute hypoxic brain injury and would predict response to therapy.MethodsSynaptopodin (SYNPO), an actin‐associated protein present in postsynaptic spines, was evaluated as a potential biomarker as well as: synaptophysin, neuron‐specific enolase, and mitochondrial cytochrome c oxidase. A secondary analysis was performed on neonatal samples collected at 8, 10, and 14 h after the initiation of therapeutic‐controlled hypothermia for acute hypoxic–ischemic encephalopathy (n = 14). Neuronal exosomes were purified from serum and protein levels were quantified using standard ELISA methods. The primary study outcomes were length of stay (LOS), discharge on seizure medication (DCMED), and composite neuroimaging score (NIS).ResultsThe slope of change in neuronal exosome SYNPO between 8 and 14 h appeared to be the most promising biomarker for all three clinical study outcomes. SYNPO was highly correlated with LOS (−0.91, P < 0.001). SYNPO increased in 6/8 without DCMED and was worse or neutral in 5/5 with DCMED (P = 0.02). All four neonates with an abnormal NIS had neutral or decreasing SYNPO (P = 0.055). Other candidate biomarkers were not associated with outcomes.InterpretationThis report provides the first clinical evidence that neural exosomes turn over rapidly enough in the peripheral circulation to be used as a “troponin‐like” test following acute brain injury. Optimal sampling and biomarkers likely vary with type of brain injury.

show abstract

“…Syndecans possess lateral heparin sulfate polysacharide chains which can be cleaved into shorter chains by heparanse activity in the endosomes (Roucourt et al, 2015). Shorter heparain sulfate chains condense and cluster leading to syndecan oligermerization, which appears to allow for syntenin binding in a cargo-dependent manner (Stoorvogel, 2015). This ultimately allows for sorting into endosomes.…”

Section: Trafficking Of Antigens Into Exosomesmentioning

confidence: 99%

The Role of HTLV-1 in Alteration of Immune Regulation Through Regulatory T Cell Dysfunction and Exosomal Manipulation

Anderson

View full text Add to dashboard Cite

Resolving sorting mechanisms into exosomes

Cited by 71 publications

References 11 publications

Exosome mediated communication within the tumor microenvironment

Exosome mediated communication within the tumor microenvironment

Diagnostic Potential of Neural Exosome Cargo as Biomarkers for Acute Brain Injury

The Role of HTLV-1 in Alteration of Immune Regulation Through Regulatory T Cell Dysfunction and Exosomal Manipulation

Contact Info

Product

Resources

About