Resolving embryonic blood cell fate choice in<i>Drosophila</i>:interplay of GCM and RUNX factors

Bataillé, Laetitia; Augé, Benoît; Ferjoux, Géraldine; Haenlin, Marc; Waltzer, Lucas

doi:10.1242/dev.02034

Cited by 70 publications

(31 citation statements)

References 33 publications

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“…Furthermore, we observed ectopic Lz expression in yki -overexpressing clones in larval tissues that do not normally express Lz expression, such as the brain, wing and leg discs (Figure S3F–S3G′ and not shown). To determine whether Yki regulates Lz at the level of transcription, we used a transgenic strain that harboured a 1.5kb fragment of the lz promoter fused to the β-galactosidase gene [31]. When yki was overexpressed with either en-Gal4 or ptc-Gal4 , we observed elevation of lz-lacZ showing that Yki indeed can regulate lz at the level of transcription (Figures 4C–4C″ and Figure S3H–S3H″).…”

Section: Resultsmentioning

confidence: 99%

The Hippo Pathway Regulates Hematopoiesis in Drosophila melanogaster

et al. 2014

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SUMMARY The Salvador-Warts-Hippo (Hippo) pathway is an evolutionarily conserved regulator of organ growth and cell fate. It performs these functions in epithelial and neural tissues of both insects and mammals, and in mammalian organs such as the liver and heart. Despite rapid advances in Hippo pathway research, a definitive role for this pathway in hematopoiesis has remained enigmatic. The hematopoietic compartments of Drosophila melanogaster and mammals possess several conserved features [1, 2]. D. melanogaster possess three types of hematopoietic cells that most closely resemble mammalian myeloid cells: plasmatocytes (macrophage-like cells), crystal cells (involved in wound healing) and lamellocytes (which encapsulate parasites). The proteins that control differentiation of these cells also control important blood lineage decisions in mammals [3–10]. Here, we define the Hippo pathway as a key mediator of hematopoiesis by showing that it controls differentiation and proliferation of the two major types of D. melanogaster blood cells, plasmatocytes and crystal cells. In animals lacking the downstream Hippo pathway kinase Warts, lymph gland cells overproliferated, differentiated prematurely and often adopted a mixed lineage fate. The Hippo pathway regulated crystal cell numbers by both cell autonomous and non-cell autonomous mechanisms. Yorkie and its partner transcription factor Scalloped were found to regulate transcription of the Runx family transcription factor, Lozenge, which is a key regulator of crystal cell fate. Further, Yorkie or Scalloped hyperactivation induced ectopic crystal cells in a non-cell autonomous, and Notch pathway-dependent fashion.

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Section: Resultsmentioning

confidence: 99%

The Hippo Pathway Regulates Hematopoiesis in Drosophila melanogaster

et al. 2014

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“…In contrast, fertility assays on transheterozygous males showed no fertility defects (data not shown). Thus, Gcm is required in reproduction in females, in addition to its well-known role in glia and blood development2430313435363738.…”

Section: Resultsmentioning

confidence: 99%

An evolutionary conserved interaction between the Gcm transcription factor and the SF1 nuclear receptor in the female reproductive system

Cattenoz¹,

Delaporte²,

Bazzi³

et al. 2016

Sci Rep

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NR5A1 is essential for the development and for the function of steroid producing glands of the reproductive system. Moreover, its misregulation is associated with endometriosis, which is the first cause of infertility in women. Hr39, the Drosophila ortholog of NR5A1, is expressed and required in the secretory cells of the spermatheca, the female exocrine gland that ensures fertility by secreting substances that attract and capacitate the spermatozoids. We here identify a direct regulator of Hr39 in the spermatheca: the Gcm transcription factor. Furthermore, lack of Gcm prevents the production of the secretory cells and leads to female sterility in Drosophila. Hr39 regulation by Gcm seems conserved in mammals and involves the modification of the DNA methylation profile of mNr5a1. This study identifies a new molecular pathway in female reproductive system development and suggests a role for hGCM in the progression of reproductive tract diseases in humans.

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“…It is already known that gcm is also expressed in hemocytes (Bernardoni et al, 1997; Bataillé et al, 2005; Waltzer et al, 2010). So, we checked whether the severe migratory delay is specific to reducing the amount of gcm in glia or in hemocytes.…”

Section: Resultsmentioning

confidence: 99%

The Glide/Gcm fate determinant controls initiation of collective cell migration by regulating Frazzled

Gupta

Kumar

Cattenoz

et al. 2016

eLife

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Collective migration is a complex process that contributes to build precise tissue and organ architecture. Several molecules implicated in cell interactions also control collective migration, but their precise role and the finely tuned expression that orchestrates this complex developmental process are poorly understood. Here, we show that the timely and threshold expression of the Netrin receptor Frazzled triggers the initiation of glia migration in the developing Drosophila wing. Frazzled expression is induced by the transcription factor Glide/Gcm in a dose-dependent manner. Thus, the glial determinant also regulates the efficiency of collective migration. NetrinB but not NetrinA serves as a chemoattractant and Unc5 contributes as a repellant Netrin receptor for glia migration. Our model includes strict spatial localization of a ligand, a cell autonomously acting receptor and a fate determinant that act coordinately to direct glia toward their final destination.DOI: http://dx.doi.org/10.7554/eLife.15983.001

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Resolving embryonic blood cell fate choice inDrosophila:interplay of GCM and RUNX factors

Cited by 70 publications

References 33 publications

The Hippo Pathway Regulates Hematopoiesis in Drosophila melanogaster

The Hippo Pathway Regulates Hematopoiesis in Drosophila melanogaster

An evolutionary conserved interaction between the Gcm transcription factor and the SF1 nuclear receptor in the female reproductive system

The Glide/Gcm fate determinant controls initiation of collective cell migration by regulating Frazzled

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