Resolvin D1 and E1 promote resolution of inflammation in microglial cells in vitro

Rey, Charlotte; Nadjar, Agnès; Buaud, Benjamin; Vaysse, Carole; Aubert, Agnès; Pallet, Véronique; Layé, Sophie; Joffre, Corinne

doi:10.1016/j.bbi.2015.12.013

Cited by 120 publications

(100 citation statements)

References 83 publications

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“…DHA oxylipins such as 7-, 10-, and 17-hydroxydocosahexaenoic acid (HDoHE), protectin D1 (PD1), resolvin D1 (RvD1), and RvD2 are negatively associated with age-related memory decline and inhibit neuronal cell death through reduction in caspase activation (Bazan, 2005;Hashimoto et al, 2015). Other oxylipins such as eicosapentaenoic acid (EPA)-derived RvE1 have antiinflammatory effects in microglial cells (Rey et al, 2016), and linoleic acid (LNA)-derived 9-hydroxyoctadecadienoic acid (HODE), 13-HODE, and 9,10-dihydroxy-octadecenoic acid are associated with increased brain oxidative stress in the aging brain (Currais et al, 2015). However, while there are reports of selected ARA, EPA, and DHA oxylipins in the brain (Chiabrando et al, 1984;Gerozissis et al, 1983;Koide et al, 1985;Morisseau et al, 2010;Naffah-Mazzacoratti et al, 1995;Schuhmann et al, 2003;Seregi and Hertting, 1984), a comprehensive profile of whole brain oxylipins is needed to identify the oxylipins present and to guide further studies on the potential functions of brain oxylipins.…”

Section: Introductionmentioning

confidence: 99%

The Brain Oxylipin Profile Is Resistant to Modulation by Dietary n‐6 and n‐3 Polyunsaturated Fatty Acids in Male and Female Rats

Ferdouse

Leng

Winter

et al. 2019

Lipids

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Oxylipins are bioactive lipids formed by the monooxygenation of polyunsaturated fatty acids (PUFA). Eicosanoids derived from arachidonic acid (ARA) are the most well‐studied class of oxylipins that influence brain functions in normal health and in disease. However, comprehensive profiling of brain oxylipins from other PUFA with differing functions, and the examination of the effects of dietary PUFA and sex differences in oxylipins are warranted. Therefore, female and male Sprague–Dawley rats were provided standard rodent diets that provided additional levels of the individual n‐3 PUFA α‐linolenic acid (ALA), eicosapentaenoic acid (EPA) or docosahexaenoic acid (DHA), or the n‐6 PUFA linoleic acid (LNA) alone or with ALA (LNA + ALA) compared to essential fatty acid‐sufficient control diets. Oxylipins and PUFA were quantified in whole brains using HPLC‐MS/MS and GC, respectively. Eighty‐seven oxylipins were present at quantifiable levels: 51% and 17% of these were derived from ARA and DHA, respectively. At the mass level, ARA and DHA oxylipins comprised 81–90% and 6–12% of total oxylipins, while phospholipid ARA and DHA represented 25–35% and 49–62% of PUFA mass, respectively. Increasing dietary n‐3 PUFA resulted in higher levels of oxylipins derived from their precursor PUFA; otherwise, the brain oxylipin profile was largely resistant to modulation by diet. Approximately 25% of oxylipins were higher in males, and this was largely unaffected by diet, further revealing a tight regulation of brain oxylipin levels. These fundamental data on brain oxylipin composition, diet effects, and sex differences will help guide future studies examining the functions of oxylipins in the brain.

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Section: Introductionmentioning

confidence: 99%

The Brain Oxylipin Profile Is Resistant to Modulation by Dietary n‐6 and n‐3 Polyunsaturated Fatty Acids in Male and Female Rats

Ferdouse

Leng

Winter

et al. 2019

Lipids

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“…DHA was shown to suppress inflammation in various cells including microglia cells [9–11], suggesting neuroinflammation is one of the multiple target mechanisms for its neuroprotective action. Recently, we have demonstrated that N -docosahexaenoylethanolamine (synaptamide), an endogenous metabolite of DHA, potently induces neurogenic differentiation of neural stem cells (NSC) and promotes neurite growth and synaptogenesis in developing neurons [12, 13].…”

Section: Introductionmentioning

confidence: 99%

N-Docosahexaenoylethanolamine ameliorates LPS-induced neuroinflammation via cAMP/PKA-dependent signaling

Park

Chen

Kevala

et al. 2016

J Neuroinflammation

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BackgroundBrain inflammation has been implicated as a critical mechanism responsible for the progression of neurodegeneration and characterized by glial cell activation accompanied by production of inflammation-related cytokines and chemokines. Growing evidence also suggests that metabolites derived from docosahexaenoic acid (DHA) have anti-inflammatory and pro-resolving effects; however, the possible role of N-docosahexaenoylethanolamine (synaptamide), an endogenous neurogenic and synaptogenic metabolite of DHA, in inflammation, is largely unknown. (The term “synaptamide” instead of “DHEA” was used for N-docosahexaenoylethanolamine since DHEA is a widely used and accepted term for the steroid, dehydroepiandrosterone.) In the present study, we tested this possibility using a lipopolysaccharide (LPS)-induced neuroinflammation model both in vitro and in vivo.MethodsFor in vitro studies, we used P3 primary rat microglia and immortalized murine microglia cells (BV2) to assess synaptamide effects on LPS-induced cytokine/chemokine/iNOS (inducible nitric oxide synthase) expression by quantitative PCR (qPCR) and enzyme-linked immunosorbent assay (ELISA). To evaluate in vivo effects, mice were intraperitoneally (i.p.) injected with LPS followed by synaptamide, and expression of proinflammatory mediators was measured by qPCR and western blot analysis. Activation of microglia and astrocyte in the brain was examined by Iba-1 and GFAP immunostaining.ResultsSynaptamide significantly reduced LPS-induced production of TNF-α and NO in cultured microglia cells. Synaptamide increased intracellular cAMP levels, phosphorylation of PKA, and phosphorylation of CREB but suppressed LPS-induced nuclear translocation of NF-κB p65. Conversely, adenylyl cyclase or PKA inhibitors abolished the synaptamide effect on p65 translocation as well as TNF-α and iNOS expression. Administration of synaptamide following LPS injection (i.p.) significantly reduced neuroinflammatory responses, such as microglia activation and mRNA expression of inflammatory cytokines, chemokine, and iNOS in the brain.ConclusionsDHA-derived synaptamide is a potent suppressor of neuroinflammation in an LPS-induced model, by enhancing cAMP/PKA signaling and inhibiting NF-κB activation. The anti-inflammatory capability of synaptamide may provide a new therapeutic avenue to ameliorate the inflammation-associated neurodegenerative conditions.Electronic supplementary materialThe online version of this article (doi:10.1186/s12974-016-0751-z) contains supplementary material, which is available to authorized users.

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“…Thus, blueberry juice significantly improved word list recall and paired associate learning in older men and women with agerelated memory decline that consumed it, relative to baseline, with paired associate learning also significantly improved relative to placebo controls. 97 A recent study 98 that measured similar cognitive tasks as those in the rodent studies, showed that freezedried blueberries (24 g/day, equivalent to one cup of fresh blueberries) for 90 days improved two measures of executive function in older adults (ages [60][61][62][63][64][65][66][67][68][69][70][71][72][73][74][75]. Participants in the blueberry group showed significantly fewer repetition errors in the California Verbal Learning test as well as reduced switch cost on a taskswitching test across study visits, relative to controls who consumed placebo powder.…”

Section: Dietary Interventions With Polyphenolic-rich Foods Can Impromentioning

confidence: 99%

“…68,69 Some of these SPMs potently modulate neuroinflammation in vivo and in vitro, through their direct effect on microglia. 70,71 DHA and SPMs are impaired at the periphery and in the brains of AD patients. 72,73 Interestingly, decreased DHA distribution in AD patient brains correlates with synaptic loss rather than amyloid beta (Aβ) deposition.…”

Section: Dietary Omega-3 Pufa Regulate Neuroinflammation and Ecbs: Romentioning

confidence: 99%

Food for thought: how nutrition impacts cognition and emotion

et al. 2017

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More than one-third of American adults are obese and statistics are similar worldwide. Caloric intake and diet composition have large and lasting effects on cognition and emotion, especially during critical periods in development, but the neural mechanisms for these effects are not well understood. A clear understanding of the cognitive-emotional processes underpinning desires to over-consume foods can assist more effective prevention and treatments of obesity. This review addresses recent work linking dietary fat intake and omega-3 polyunsaturated fatty acid dietary imbalance with inflammation in developing, adult, and aged brains. Thus, early-life diet and exposure to stress can lead to cognitive dysfunction throughout life and there is potential for early nutritional interventions (e.g., with essential micronutrients) for preventing these deficits. Likewise, acute consumption of a high-fat diet primes the hippocampus to produce a potentiated neuroinflammatory response to a mild immune challenge, causing memory deficits. Low dietary intake of omega-3 polyunsaturated fatty acids can also contribute to depression through its effects on endocannabinoid and inflammatory pathways in specific brain regions leading to synaptic phagocytosis by microglia in the hippocampus, contributing to memory loss. However, encouragingly, consumption of fruits and vegetables high in polyphenolics can prevent and even reverse age-related cognitive deficits by lowering oxidative stress and inflammation. Understanding relationships between diet, cognition, and emotion is necessary to uncover mechanisms involved in and strategies to prevent or attenuate comorbid neurological conditions in obese individuals.

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Resolvin D1 and E1 promote resolution of inflammation in microglial cells in vitro

Cited by 120 publications

References 83 publications

The Brain Oxylipin Profile Is Resistant to Modulation by Dietary n‐6 and n‐3 Polyunsaturated Fatty Acids in Male and Female Rats

The Brain Oxylipin Profile Is Resistant to Modulation by Dietary n‐6 and n‐3 Polyunsaturated Fatty Acids in Male and Female Rats

N-Docosahexaenoylethanolamine ameliorates LPS-induced neuroinflammation via cAMP/PKA-dependent signaling

Food for thought: how nutrition impacts cognition and emotion

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