Resolution of the fluorescence bands in greening chloroplasts of maize

Garab, Győző; Horváth, Gábor; Faludi-Dániel, Ágnes

doi:10.1016/s0006-291x(74)80288-3

Cited by 12 publications

(10 citation statements)

References 10 publications

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“…Low-temperature fluorescence emission spectra were measured according to Garab et al (1974) using a custom-built spectrofluorimeter. Samples for fluorescence measurements were preparated by cutting off the middle part of the incubated leaf segment, placing it on the sample holder, and immersing in liquid N 2.…”

Section: Methodsmentioning

confidence: 99%

Formation of the photosynthetic apparatus during greening of cadmium-poisoned barley leaves

Horváth¹,

Droppa

Oravecz³

et al. 1996

Planta

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Abstract. The effect of cadmium on the formation of the photosynthetic apparatus of greening barley (Hordeum vulgare L. cv. Triangel) leaves has been investigated. Cadmium treatment of dark-grown leaves strongly reduced the extent of chlorophyll accumulation during greening. Low-temperature fluorescence emission showed, however, that neither the synthesis nor photoconversion of protochlorophyllide was inhibited, although a blue shift of the main fluorescence emission from 685 to 668 mm was found. Chlorophyll fluorescence lifetime was followed by measuring the phase-shift angle of modulated emission. Whereas this parameter normally decreases rapidly during greening, this change proceeded noticeably slower with increasing severity according to cadmium concentration. Cadmium also decreased the variable part of fluorescence induction. These results suggest that the cadmium in greening leaves, rather than interfering with chlorophyll biosynthesis, acts mainly by disturbing the integration of chlorophyll molecules into the stable complexes required for normal functional photoysnthetic activity.

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Section: Methodsmentioning

confidence: 99%

Formation of the photosynthetic apparatus during greening of cadmium-poisoned barley leaves

Horváth¹,

Droppa

Oravecz³

et al. 1996

Planta

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“…MV 651) were grown in greenhouse for 8-10 days. Chloroplasts were isolated from the mesophyll of the first leaves as previously described [3] . Stroma membranes and grana were prepared from the chloroplasts by ultrasonication and differential centrifugation [4].…”

Section: Methodsmentioning

confidence: 99%

Chlorophyll forms affected by 3(3,4‐dichlorophenyl)‐1,1‐dimethylurea as shown by low temperature fluorescence spectra of chloroplasts and fragments

et al. 1976

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“…This hypothesis was based originally almost entirely upon evidence from the deconvolution of absorption spectra (Gulyaev and Litvin, 1967;Butler and Hopkins, 1970;French et al, 1972). Garab et al (1974) measured fluorescence emission spectra at -196°C of chloroplasts from greening maize and also digitonin-treated mature chloroplasts. They found evidence for six forms fluorescing from 675 to 738 nm.…”

Section: Biotogical Systemsmentioning

confidence: 99%

“…Faludi-Daniel and Breton (1975) compared the linear dichroism spectra of mesophyll and bundlesheath maize chloroplasts and observed differences that could be attributed to varying proportions of the Chl a forms. Garab and Breton (1976) have measured the spectrum at -140°C of the polarized fluorescence emission of magnetically oriented spinach chloroplasts. They found evidence for the existence of at least five different fluorescing species.…”

Section: Biotogical Systemsmentioning

confidence: 99%

Spectroscopy of Chlorophyll in Biological and Synthetic Systems*

Brown

1977

Photochem Photobiol

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Abstract-This review discusses recent spectroscopic studies aimed at discovering the structure, orientation, and function of chlorophyll in u i w . In plant membranes there appear to be at least two distinct types of chlorophyll a. The greater part, over 9Y%, is antenna chlorophyll which absorbs and transfers radiant energy to a few specialized chlorophyll molecules in a reaction center where the actual charge separation occurs.A dimer-oligomer model for antenna chlorophyll has been proposed on the basis of comparative studies of the absorption spectra of chlorophyll in various dry solvents and in uiw. Unfortunately a similarity between essentially structureless broad spectra is very weak evidence for their original identity. Also the requirement of an anhydrous environment for most of the chlorophyll in biological material is an unlikely postulate. A cross-linked, linear polymer model of chlorophyll in oiuu has also been proposed. Recent Resonance Raman spectroscopic results appear to rule out, in large part, either polymer model and once again suggest that it is the various attachments of chlorophyll to proteins which determine its function as antenna pigment in uiw. Circular dichroism measurements of chlorophyll in various plant materials have also led to the conclusion that antenna chlorophyll has strong interaction with protein. However, some doubt still exists as to the interpretation of these CD results. New studies of fluorescence, polarized fluorescence and Resonance Raman spectroscopy of various plant species corroborate the original proposition, based upon deconvolution of absorption spectra, that antenna chlorophyll occurs in uiuo in at least five discrete pools, and that each pool is likely to be located in the same environment in different plants.A new model-systems approach to simulating chlorophyll in uiuo has come through the use of lipid bilayers and liposomes. Charge transfer has been observed between chlorophyll in a lipid phase and phycobiliproteins or cytochrome c.The most promising, newly synthesized model for the reaction center, P700, is a covalently bound dimeric derivative of pyrochlorophyllide a. Its properties are similar to P700 in several respects except for reversible photooxidation which has not yet been observed. By detergent treatments chlorophyllprotein complexes having about 20-40 chlorophyll a molecules for every P700 have been isolated from different plants, and their spectroscopic properties are under investigation in several laboratories. The several hypotheses to explain the shape of the oxidized minus reduced absorption difference spectrum of P700 have not yet been reconciled. The nature of the photosystem I1 reaction center chlorophyll, P680, is also a subject of active investigation. Its absorption difference spectrum appears to have two kinetic components.

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Resolution of the fluorescence bands in greening chloroplasts of maize

Cited by 12 publications

References 10 publications

Formation of the photosynthetic apparatus during greening of cadmium-poisoned barley leaves

Formation of the photosynthetic apparatus during greening of cadmium-poisoned barley leaves

Chlorophyll forms affected by 3(3,4‐dichlorophenyl)‐1,1‐dimethylurea as shown by low temperature fluorescence spectra of chloroplasts and fragments

Spectroscopy of Chlorophyll in Biological and Synthetic Systems*

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