Resolution of the Facilitated Transport of Dehydroascorbic Acid from Its Intracellular Accumulation as Ascorbic Acid

Vera, Juan Carlos; Rivas, Coralia I.; Velásquez, Fernando V.; Zhang, Ronɡ Hua; Concha, Ilona I.; Golde, David W.

doi:10.1074/jbc.270.40.23706

Cited by 158 publications

(203 citation statements)

References 51 publications

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“…Uptake Assays-The uptake experiments were carried out as previously described using 3-day-old cell monolayers in 12-well tissue culture plates (8,15). The transport medium contained 15 mM Hepes buffer (pH 7.6), 135 mM NaCl, 5 mM KCl, 1.8 mM CaCl 2 , 0.8 mM MgCl 2 , and 0.1 mM dithiothreitol.…”

Section: Methodsmentioning

confidence: 99%

Mechanistic Insights and Functional Determinants of the Transport Cycle of the Ascorbic Acid Transporter SVCT2

Godoy

Ormazábal

Moraga-Cid

et al. 2007

Journal of Biological Chemistry

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102

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Section: Methodsmentioning

confidence: 99%

Mechanistic Insights and Functional Determinants of the Transport Cycle of the Ascorbic Acid Transporter SVCT2

Godoy

Ormazábal

Moraga-Cid

et al. 2007

Journal of Biological Chemistry

Self Cite

102

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“…Evidence obtained from expression studies in Xenopus laevis oocytes indicates that GLUT1, GLUT2, GLUT3, and GLUT4 are dehydroascorbic acid transporters (Vera et al, 1993;Vera et al, 1995;Rumsey et al, 1997). Transport of dehydroascorbic acid via glucose transporters is kinetically and biologically separable from reduction and accumulation of this substrate (Vera et al, 1995;Rivas et al, 1997).…”

Section: Vitamin C and Vitamin C Transportersmentioning

confidence: 99%

“…Transport of dehydroascorbic acid via glucose transporters is kinetically and biologically separable from reduction and accumulation of this substrate (Vera et al, 1995;Rivas et al, 1997).…”

Section: Vitamin C and Vitamin C Transportersmentioning

confidence: 99%

“…Fourteen GLUT isoforms have now been identifi ed in the human genome. GLUT1 transports L-dehydroascorbic acid, an oxidized intermediate of ascorbic acid (Vera et al, 1993;Vera et al, 1995;Rumsey et al, 1997). Dehydroascorbic acid entry via GLUT family transporters was initially investigated because of the structural similarities between dehydroascorbic acid and glucose.…”

Section: Vitamin C and Vitamin C Transportersmentioning

confidence: 99%

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Vitamin C and oxidative stress in the seminiferous epithelium

et al. 2011

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In this article, we focus on the fundamental role of vitamin C transporters for the normal delivery of vitamin C to germ cells in the adluminal compartment of seminiferous tubules. We argue that the redox status within spermatozoa or in semen is partly responsible for the etiology of infertility. In this context, antioxidant defence plays a critical role in male fertility. Vitamin C, a micronutrient required for a wide variety of metabolic functions, has long been associated with male reproduction. Two systems for vitamin C transport have been described in mammals. Facilitative hexose transporters (GLUTs), with 14 known isoforms to date, GLUT1-GLUT14, transport the oxidized form of vitamin C (dehydroascorbic acid) into the cells. Sodium ascorbic acid co-transporters (SVCTs), SVCT1 and SVCT2 transport the reduced form of vitamin C (ascorbic acid). Sertoli cells control germ cell proliferation and differentiation through cell-cell communication and form the blood-testis barrier. Because the blood-testis barrier limits direct access of molecules from the plasma into the adluminal compartment of the seminiferous tubule, one important question is the method by which germ cells obtain vitamin C. Some interesting results have thrown light on this matter. Expression of SVCT2 and some isoforms of GLUT transporters in the testis have previously been described. Our group has demonstrated that Sertoli cells express functionally active vitamin C transporters. Kinetic characteristics were described for both transport systems (SVCT and GLUT systems). Sertoli cells are able to transport both forms of vitamin C. These fi ndings are extremely relevant, because Sertoli cells may control the amount of vitamin C in the adluminal compartment, as well as regulating the availability of this metabolite throughout spermatogenesis.

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“…To verify the different transport mechanisms of AA and DHA, the effects of Na + , of hexose-transport competitors (glucose and deoxyglucose) [24] and of a specific hexose-transport inhibitor (cytochalasin B) [25] on the vitamin C transport were examined. AA uptake was Na + -dependent although unaffected by hexosetransport inhibitors\competitors, whereas DHA uptake was Na + - independent although affected by cytochalasin B and deoxyglucose (Table 1).…”

Section: Dha and Aa Transportmentioning

confidence: 99%

Dehydroascorbic acid uptake in a human keratinocyte cell line (HaCaT) is glutathione-independent

Savini

Duflot

Avigliano³

2000

Biochem. J.

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Resolution of the Facilitated Transport of Dehydroascorbic Acid from Its Intracellular Accumulation as Ascorbic Acid

Cited by 158 publications

References 51 publications

Mechanistic Insights and Functional Determinants of the Transport Cycle of the Ascorbic Acid Transporter SVCT2

Mechanistic Insights and Functional Determinants of the Transport Cycle of the Ascorbic Acid Transporter SVCT2

Vitamin C and oxidative stress in the seminiferous epithelium

Dehydroascorbic acid uptake in a human keratinocyte cell line (HaCaT) is glutathione-independent

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