“…The fluorescent coenzyme is a natural biomolecule that is present in a small fraction of proteins, such as dihydronicotinamide adenine dinucleotide, flavin adenine dinucleotide (FAD) and flavin mononucleotide (FMN) ( Su, Hung, Huang, & Lin, 2011 ). Another class of chromophore is extrinsic fluorophore that can covalently and non-covalently bind with protein unique active sites, with the most prominent being 1-anilino-8-naphthalene sulfonate (ANS).…”