1968
DOI: 10.1021/ac60264a059
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Resolution of racemic aspartic acid, tryptophan, hydroxy and sulfhydryl amino acids by gas chromatography

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Cited by 39 publications
(8 citation statements)
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“…Only by sacrificing resolution of D-leucine from proline can this interference (with the NTFA derivatives) be avoided. The alternative that we have employed is to destroy the TFA serine derivative by addition of methanol to the completed derivative followed by refrigerated overnight storage (Pollock and Kawauchi, 1968). By following this procedure, good agreement between isopropyl and butanol has been observed ( Table 2, data for laboratory E).…”
Section: Resultsmentioning
confidence: 99%
“…Only by sacrificing resolution of D-leucine from proline can this interference (with the NTFA derivatives) be avoided. The alternative that we have employed is to destroy the TFA serine derivative by addition of methanol to the completed derivative followed by refrigerated overnight storage (Pollock and Kawauchi, 1968). By following this procedure, good agreement between isopropyl and butanol has been observed ( Table 2, data for laboratory E).…”
Section: Resultsmentioning
confidence: 99%
“…Like phosphatidylethanolamine, phosphatidylserine forms a dinitrobenzamide which possesses excellent solvent partition properties especially following further methylation (4), but this derivative is difficult to recover from the reaction mixture when present in small amounts. Much more readily prepared and re-Ipresented in part at the JOCS-AOCS Joint Meeting, Los Angeles, 1972. covered are the trifluoroacetamides of phosphatidylserine in which the polar amino group is masked resulting in greatly improved chromatographic properties, as already demonstrated for amino alcohols (5) and amino acids (6,7). As a background for studies of the metabolism of molecular species, the present report describes the preparation of trifluoroacetamides from synthetic and natural phosphatidylserines and phosphatidylethanolamines along with their separation into different classes of unsaturation by AgNO3-TLC.…”
Section: Introductionmentioning
confidence: 83%
“…After standing 30 min, the O-PFP group was removed, and the sample was evaporated in an ice bath to remove the methanol and methylene chloride. This pyridine-methanol accelerated removal of the O-PFP group was used in preference to that described in (14) because the O-PFP group does not transesterify very rapidly with methanol alone as does O-TFA groups. The O-acetylation of the free hydroxyl, N-PFP amino acid ester can, however, be accomplished using the procedure described in ( 14), and the final derivative diluted with methylene chloride for analysis.…”
Section: Methodsmentioning
confidence: 99%
“…With this background, and the fact that geochemists have proposed in recent years that the rate of racemization of amino acids might be used as a method for age dating or geothermometry (10)(11)(12), we decided to reinvestigate the content of D-amino acids in soils by using a gas chromatographic technique which would enable us to determine more specifically the identity of these isomers (13)(14)(15). This method involves esterification of the amino acids with (+)-2-butanol, followed by acylation of the remaining functional groups with trifluoroacetic anhydride (TFA) or other perfluoroalkanoyl anhy-tiride.…”
mentioning
confidence: 99%