Resolution of overlapping chromatographic peaks with an interfering background

Software-Based Deconvolution of Gas Chromatograms: An Experiment for the Instrumental Analysis Laboratory

“…For example, when NLR is used to deconvolute overlapped chromatographic peaks an equation containing terms that adequately describe the shape of each individual peak must be used. With the function so selected the P/s can usually be used to construct the individual peaks upon convergence (1)(2)(3)(4)(5)(6)(7)(8).…”

“…The second function investigated for use in NLR described the shape of each peak as a simple Gaussian. This peak shape has been used by many workers in deconvolutions of overlapped chromatographic peaks by NLR (3)(4)(5)(6)(7)(8). The Gaussian function described by Westerberg (3) was used and can be written for two overlapped peaks as…”

“…The first step in constructing the reducible representation is to determine the number of atoms that remain unshifted nu(R) after each symmetry operation R is carried out. Jolly provides a table of values for character contributions /IR) for all symmetry operations (5). The character contribution for a symmetry operation flR) times the number of unshifted atoms following a symmetry operation nu(R) is the part of the reducible representation '/(R) for that symmetry operation.…”

“…An approach that has received considerable attention for gas chromatography and high-performance liquid chromatography involves partial resolution of the components chromatographically followed by completing the deconvolution using nonlinear regression analysis to fit the chromatographic data to an equation that describes the overlapped peaks (1)(2)(3)(4)(5)(6)(7)(8). In many cases, this approach provides an accurate analysis of poorly resolved components without chromatographic optimization, and it can increase sample throughput.…”

Aspects of High-Resolution Gas Chromatography as Applied to the Analysis of Hydrocarbon Fuels and Other Complex Organic Mixtures. Volume 1

“…The time base can be condensed so that the entire tracing can be reproduced on a single sheet of paper.Since the raw GC data are stored on tape or disk, it is possible to go back later and look at any portion of the GC tracing in detail. Through an exacting examination of each of the various recorded elution profiles, the detection of merged solute zones[119][120][121] should be possible. Of course, by having the raw HRGC data in storage, numerous computer processing and data handling techniques could be applied at any time in the future.5.…”