Resolution of neutrophilic inflammation by H2O2 in antigen‐induced arthritis

Lopes, Fernando; Coelho, Fernanda; Costa, Vivian Vasconcelos; Vieira, Eliane; Sousa, Lirlândia P.; Silva, Tarcı́lia Aparecida; Vieira, Leda Quércia; Teixeira, Mauro Martins; Pinho, Vanessa

doi:10.1002/art.30448

Cited by 44 publications

(59 citation statements)

References 49 publications

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“…There is growing recognition that oxidants play significant anti-inflammatory roles in inflammation (12,14,49), although the exact mechanisms have not been elucidated. We have previously shown that lyso-PS was the predominant modified PS species identified in activated neutrophils, and although roles for several phospholipases in its production were ruled out, an absolute requirement of an active NADPH oxidase was demonstrated (16,17).…”

Section: Discussionmentioning

confidence: 99%

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Neutrophils Regulate Tissue Neutrophilia in Inflammation via the Oxidant-modified Lipid Lysophosphatidylserine

Frasch

Fernandez-Boyanapalli

Berry

et al. 2013

Journal of Biological Chemistry

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Background: NADPH oxidase-generated lyso-PS enhances efferocytosis by macrophages. Results: Recruited, viable lyso-PS high neutrophils are readily cleared by macrophages in vitro and in vivo via G2A signaling. Lyso-PS high neutrophils also program macrophages to a highly efferocytic resolving state. Conclusion: Lyso-PS in/on exudate neutrophils orchestrates early removal of neutrophils and subsequent macrophage programming. Significance: Neutrophil-generated lyso-PS regulates tissue neutrophilia of acute inflammation.

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Section: Discussionmentioning

confidence: 99%

“…Neutrophil-generated oxidants, in addition to microbial killing and inciting inflammation, may also play somewhat paradoxical anti-inflammatory roles (11,12). Indeed, deficient func-tioning of the NADPH oxidase is associated with exaggerated inflammation in human and murine models of chronic granulomatous disease (CGD) (13)(14)(15).…”

mentioning

confidence: 99%

Neutrophils Regulate Tissue Neutrophilia in Inflammation via the Oxidant-modified Lipid Lysophosphatidylserine

Frasch

Fernandez-Boyanapalli

Berry

et al. 2013

Journal of Biological Chemistry

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“…Defective leukocyte apoptosis and subsequent removal of apoptotic cells by phagocytes is thought to be important for the initiation and propagation of chronic inflammation. The role of NADPH oxidase-derived H 2 O 2 to induce apoptosis of phagocytes and resolution of inflammation has been reported in a model of antigen-induced arthritis [65] . It is possible to take advantage of this function of H 2 O 2 to regulate pathogenic inflammation in chronic wounds.…”

Section: Hydrogen Peroxide: Implications For Chronic Wound Treatmentmentioning

confidence: 99%

Hydrogen Peroxide: A Potential Wound Therapeutic Target

Zhu¹,

Wang²,

Lu³

et al. 2017

Med Princ Pract

109

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Hydrogen peroxide (H2O2) is a topical antiseptic used in wound cleaning which kills pathogens through oxidation burst and local oxygen production. H2O2 has been reported to be a reactive biochemical molecule synthesized by various cells that influences biological behavior through multiple mechanisms: alterations of membrane potential, generation of new molecules, and changing intracellular redox balance, which results in activation or inactivation of different signaling transduction pathways. Contrary to the traditional viewpoint that H2O2 probably impairs tissue through its high oxidative property, a proper level of H2O2 is considered an important requirement for normal wound healing. Although the present clinical use of H2O2 is still limited to the elimination of microbial contamination and sometimes hemostasis, better understanding towards the sterilization ability and cell behavior regulatory function of H2O2 within wounds will enhance the potential to exogenously augment and manipulate healing.

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“…The total number of leukocytes was determined by counting in a Neubauer chamber after staining with Turk's solution. Differential counts were obtained from cytospin (Shandon III, Thermo Shandon, Frankfurt, Germany) preparations by evaluating the percentage of each class of leukocyte based on nuclear morphology following staining with May-Grünwald-Giemsa [15].…”

Section: Lps-induced Neutrophil Recruitment To Mouse Kneementioning

confidence: 99%

A Trypsin-Sensitive Proteoglycan from the Tapeworm Hymenolepis diminuta Inhibits Murine Neutrophil Chemotaxis in vitro by Suppressing p38 MAP Kinase Activation

et al. 2018

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It has emerged that neutrophils can play important roles in the host response following infection with helminth parasites. Mice infected with the tapeworm, Hymenolepis diminuta, are protected from some inflammatory conditions, accompanied by reduced neutrophil tissue infiltration. Thus, the ability of a phosphate-buffered saline-soluble extract of the worm (H. diminuta extract [HdE]) was tested for (1) its ability to activate murine neutrophils (Ca²⁺ mobilization, reactive oxygen species (ROS) and cytokine production); and (2) affect neutrophil chemotaxis in vitro to the penta-peptide, WKYMVm, the chemokine, KC, and leukotriene B₄. HdE was not cytotoxic to neutrophils, elicited a Ca²⁺ response and ROS, but not, cytokine (KC, interleukin-10, tumour necrosis factor-α) generation. HdE is not a chemotactic stimulus for murine neutrophils. However, a heat- and trypsin-sensitive, acid-insensitive proteoglycan (sensitive to sodium metaperiodate) in the HdE significantly reduced neutrophil chemotaxis towards WKYMVm or KC, but not LTB₄. The latter suggested that the HdE interfered with p38 mitogen-activated protein kinase signalling, which is important in WKYMVm chemotaxis. Corroborating this, immunoblotting revealed reduced phosphorylated p38, and the downstream signal heat-shock protein-27, in protein extracts from HdE + WkYMVm treated cells compared to those exposed to the penta-peptide only. We speculate that HdE can be used to modify the outcome of neutrophilic disease and that purification of the bioactive proteoglycan(s) from the extract could be used as a template to design immunomodulatory drugs targeting neutrophils.

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Resolution of neutrophilic inflammation by H₂O₂ in antigen‐induced arthritis

Cited by 44 publications

References 49 publications

Neutrophils Regulate Tissue Neutrophilia in Inflammation via the Oxidant-modified Lipid Lysophosphatidylserine

Neutrophils Regulate Tissue Neutrophilia in Inflammation via the Oxidant-modified Lipid Lysophosphatidylserine

Hydrogen Peroxide: A Potential Wound Therapeutic Target

A Trypsin-Sensitive Proteoglycan from the Tapeworm <b><i>Hymenolepis diminuta</i></b> Inhibits Murine Neutrophil Chemotaxis in vitro by Suppressing p38 MAP Kinase Activation

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