Resolution of infrared matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry using glycerol; enhancement with a disperse laser beam

Jung, Hyun Suk; Moon, Jeong Hee; Kim, Myoung Soo

doi:10.1002/rcm.2983

Cited by 3 publications

(2 citation statements)

References 29 publications

(35 reference statements)

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“…A schematic drawing of its ion source is shown in Figure 1. Electrode layout and use of a 1.5 mm diameter hemispherical cavity on the sample plate for sample loading were the same as in a previous design [21] except that the cavity was not covered with a grid. The sample plate was cooled by cold ethanol from a thermostat (cc815 vpc, Huber, Offenburg, Germany).…”

Section: Methodsmentioning

confidence: 99%

Expansion Cooling in the Matrix Plume is Under-Recognized in MALDI Mass Spectrometry

Bae

Moon²,

Kim

2011

J. Am. Soc. Mass Spectrom.

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Time-of-flight (TOF) mass spectra for a peptide (Y(6)) were obtained by utilizing matrix-assisted infrared laser desorption ionization (IR-MALDI) with glycerol as the matrix and by ultraviolet MALDI with α-cyano-4-hydroxycinnamic acid (CHCA), sinapinic acid (SA), and 2,5-dihydroxybenzoic acid (DHB). Collisional activation during ion extraction and exothermicity in the gas-phase proton transfer were found to be unimportant as the driving forces for in-source (ISD) and post-source (PSD) decays, indicating that the thermal energy acquired during photo-ablation is responsible for their occurrence. The temperatures of [Y(6) + H](+) in the 'early' and 'late' matrix plumes were estimated by the kinetic analysis of the ISD and PSD yields, respectively. The order of the temperatures was glycerol < DHB ≈ SA < CHCA in the early plume and glycerol < DHB < SA < CHCA in the late plume. For each matrix, the temperature in the late plume was lower than in the early plume by 300-400 K, which was attributed to expansion cooling. The model (thermalization followed by expansion cooling) proposed to explain the occurrence of both rapid ISD and slow PSD is not only in sharp contrast with but also mutually exclusive with the prevailing explanation that the exothermicity in proton transfer and in-plume collisional activation are the driving forces for ion fragmentation in MALDI. The model also explains why MALDI is more successful for mass spectrometry of labile molecules than other desorption techniques that do not utilize a matrix. Factors affecting the plume temperature are also discussed.

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Section: Methodsmentioning

confidence: 99%

Expansion Cooling in the Matrix Plume is Under-Recognized in MALDI Mass Spectrometry

Bae

Moon²,

Kim

2011

J. Am. Soc. Mass Spectrom.

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“…This is particularly true in UV-MALDI. IR-MALDI, on the other hand, is generally considered [7,21,22] to induce significantly less fragmentation than UV-MALDI, particularly for fragile analytes such as oligonucleotides [23]. The time scale for metastable ion fragmentation in MALDI is such that much of the fragmentation occurs after analyte ions have left the ion source and so is referred to as post-source metastable ion decay (PSD) [8,15,19].…”

Section: Introductionmentioning

confidence: 99%

Wavelength dependence on the level of post-source metastable ion decay observed in infrared matrix-assisted laser desorption ionization

Durrant

Brown

2009

International Journal of Mass Spectrometry

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The levels of post-source metastable ion decay (PSD) observed in several peptides and proteins ionized by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI TOF-MS) are measured utilizing both infrared (IR) and ultraviolet (UV) desorption wavelengths. A gridless deceleration ion optic is employed to temporally separate stable analyte ions from analyte metastable neutral and ion fragments. Comparisons of the extent of PSD that is observed in UV-MALDI at 337 nm and IR-MALDI at multiple wavelengths between 2.8 and 3.0 μm are made using the same matrices and analytes. The amount of PSD observed using IR-MALDI was found to be highly dependent on the specific IR wavelength (2.8-3.0 μm) employed for desorption. IR wavelengths shorter than 2.86 μm tended to produce higher levels of PSD, while longer IR wavelengths typically produced significantly less PSD when using a number of common MALDI matrices. Relative PSD levels are quantified by determining the percentage of the neutral fragment signal intensity to the intensity of the stable singly protonated molecular species observed in decelerated MALDI spectra. These studies suggest that an analyte ion activation pathway leading to significant PSD in IR-MALDI may proceed by way of vibrational excitation of the analyte molecules during the desorption event.

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Current literature in mass spectrometry

2007

J. Mass Spectrom.

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In order to keep subscribers up‐to‐date with the latest developments in their field, John Wiley & Sons are providing a current awareness service in each issue of the journal. The bibliography contains newly published material in the field of mass spectrometry. Each bibliography is divided into 11 sections: 1 Reviews; 2 Instrumental Techniques & Methods; 3 Gas Phase Ion Chemistry; 4 Biology/Biochemistry: Amino Acids, Peptides & Proteins; Carbohydrates; Lipids; Nucleic Acids; 5 Pharmacology/Toxicology; 6 Natural Products; 7 Analysis of Organic Compounds; 8 Analysis of Inorganics/Organometallics; 9 Surface Analysis; 10 Environmental Analysis; 11 Elemental Analysis. Within each section, articles are listed in alphabetical order with respect to author (4 Weeks journals ‐ Search completed at 11th. Apr. 2007)

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Resolution of infrared matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry using glycerol; enhancement with a disperse laser beam

Cited by 3 publications

References 29 publications

Expansion Cooling in the Matrix Plume is Under-Recognized in MALDI Mass Spectrometry

Expansion Cooling in the Matrix Plume is Under-Recognized in MALDI Mass Spectrometry

Wavelength dependence on the level of post-source metastable ion decay observed in infrared matrix-assisted laser desorption ionization

Current literature in mass spectrometry

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