“…Later, the Whelk-O1 phase proved a broad-spectrum CSP for the separation of compounds bearing an aromatic system with a hydrogen-bond acceptor group located near the stereogenic center . It has been, thereafter, used in the last 20 years for the separation of many chiral compounds whose structure fits the general mechanism of separation, including alcohols, amides, esters, ethers, epoxides, carbamates, aldehydes, ketones, carboxylic acids, aziridines, phosphonates, and ureas. − The phase is commonly used under normal-phase (NP) conditions but interesting examples have been reported with water-rich mobile phases, , as well as in polar organic mode (POM) . Further applications include the use of supercritical/subcritical CO 2 solvent, − while its high loading capacity has been exploited in preparative LC. , The Whelk-O1 selector has also been used in Inverted Chirality Columns Approach to determine the enantiomeric excess of ( S )-namitecan (a water-soluble camptothecin derivative) in the absence of the minor enantiomer or the racemate as reference material …”