Resistivity of Leech Retzius Nerve Cells to Long-Lasting Oxidant

Jovanović, Zorica; Beleslin, B

doi:10.1007/978-1-4615-5405-9_165

Cited by 5 publications

(4 citation statements)

References 12 publications

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“…in addition, H 2 o 2 is relatively stable and poorly reactive in the absence of transition metals [12]. However, previous studies have shown that a 1 mM H 2 o 2 treatment with 0.01 mM Fecl 2 did not significantly change the resting membrane potential of LRncs [18]. Recent studies have suggested that limiting H 2 o 2 diffusion into cells has a key role in the protection against extracellular H 2 o 2 .…”

Section: Discussionmentioning

confidence: 99%

A Comparison of the Effects of Cumene Hydroperoxide and Hydrogen Peroxide on Retzius Nerve Cells of the Leech <i>Haemopis sanguisuga</i>

Jovanović

2013

Exp. Anim.

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Abstract:Oxidative stress and the production of reactive oxygen species are known to play a major role in neuronal cell damage, but the exact mechanisms responsible for neuronal injury and death remain uncertain. In the present study, we examined the effects of oxidative stress on spontaneous spike activity and depolarizing outward potassium current by exposing the Retzius neurons of the leech to cumene hydroperoxide (CHP) and hydrogen peroxide (H 2 O 2 ), the oxidants commonly used to examine oxidative mechanisms mediating cell death. We observed that relatively low concentrations of CHP (0.25, 1, and 1.5 mM) led to a marked prolongation of spontaneous repetitive activity. The prolonged action potentials showed an initial, spike-like depolarization followed by a plateau phase. In contrast, H 2 O 2 at the same and much higher concentrations (0.25 to 5 mM) did not significantly change the duration of spontaneous spike potentials of leech Retzius nerve cells (LRNCs). In the voltage clamp experiments, calcium-activated outward potassium currents, needed for the repolarization of the action potential, were suppressed with CHP, but not with H 2 O 2 . The present findings indicate that CHP is a more potent oxidant and neurotoxin than H 2 O 2 and that the effect of CHP on the electrophysiological properties of LRNCs may be due to the inhibition of the potassium channels.

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Section: Discussionmentioning

confidence: 99%

A Comparison of the Effects of Cumene Hydroperoxide and Hydrogen Peroxide on Retzius Nerve Cells of the Leech <i>Haemopis sanguisuga</i>

Jovanović

2013

Exp. Anim.

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“…In the voltage clamp experiments, H 2 O 2 was ineffective in the supression of fast and slow Ca 2+ activated K + currents. The background of this study were the findings that a 1 mM H 2 O 2 treatment with or without FeCl 2 did not significantly change the resting membrane potential of LRNC (Jovanović & Beleslin, 1996;Jovanović & Beleslin, 1997;Jovanović & Beleslin, 2004). …”

Section: Discussionmentioning

confidence: 90%

Effects of Oxidative Stress on the Electrophysiological Function of Neuronal Membranes

Jovanović¹

2012

Oxidative Stress and Diseases

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“…Ovaj podatak ukazuje da u ovom modelu oksidativnog stresa OH · nije generisan, ili da ganglijske ćelije pijavice imaju efikasan antioksidativni sistem za detoksikaciju H 2 O 2 [33,34]. S druge strane, potvrđeno je da je organski peroksid, kumen hidroperoksid efikasniji neurotoksin i oksidans od H 2 O 2 , a da je ovaj efekat potenciran sa bakrom [35][36][37].…”

Section: Oksidativni I Antioksidativni Potencijal Neurona I Glijalnihunclassified

Resistance of nerve cells to oxidative injury

Jovanović

2011

Med pregl

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Resistivity of Leech Retzius Nerve Cells to Long-Lasting Oxidant

Cited by 5 publications

References 12 publications

A Comparison of the Effects of Cumene Hydroperoxide and Hydrogen Peroxide on Retzius Nerve Cells of the Leech <i>Haemopis sanguisuga</i>

A Comparison of the Effects of Cumene Hydroperoxide and Hydrogen Peroxide on Retzius Nerve Cells of the Leech <i>Haemopis sanguisuga</i>

Effects of Oxidative Stress on the Electrophysiological Function of Neuronal Membranes

Resistance of nerve cells to oxidative injury

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