Resistive-pulse measurements with nanopipettes: detection of Au nanoparticles and nanoparticle-bound anti-peanut IgY

Wang, Yixian; Keçeci, Kaan; Mirkin, Michael V.; Mani, Vigneshwaran; Sardesai, Naimish P.; Rusling, James F.

doi:10.1039/c2sc21502k

Cited by 94 publications

(140 citation statements)

References 55 publications

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“…14,15,18,19 Under these conditions, the diffuse double layer is expected to be compressed to a sub-nanometer scale, and therefore undetectable, level according to: 50…”

Section: Characterization Of Nanopipettes In High Ionic Strength Mediamentioning

confidence: 99%

“…Nanoelectrodes can also be fabricated by electrochemically plating nanopipettes with a variety of different metals. 12 Applications of nanopipettes include as tools for sizing, counting, [13][14][15][16][17] and sequencing of single particles or molecules. [18][19][20] These applications use the changes in ionic current through the end of the nanopipette (with an applied bias), as a single entity passes through the end of the probe, to provide diagnostic information.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of Nanopipettes

et al. 2016

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Nanopipettes are widely used in electrochemical and analytical techniques as tools for sizing, sequencing, sensing, delivery and imaging. For all of these applications, the response of a nanopipette is strongly affected by its geometry and surface chemistry. As the size of nanopipettes becomes smaller, precise geometric characterization is increasingly important, especially if nanopipette probes are to be used for quantitative studies and analysis. This contribution highlights the combination of data from voltage-scanning ion conductivity experiments, transmission electron microscopy (TEM) and finite element method (FEM) simulations to fully characterize nanopipette geometry and surface charge characteristics, with an accuracy not achievable using existing approaches. Indeed, it is shown that presently used methods for nanopipette characterization can lead to highly erroneous information on nanopipettes. The new approach to characterization further facilitates high-level quantification of the behavior of nanopipettes in electrochemical systems, as demonstrated herein for a scanning ion conductance microscope (SICM) setup.

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“…14,15,18,19 Under these conditions, the diffuse double layer is expected to be compressed to a sub-nanometer scale, and therefore undetectable, level according to: 50…”

Section: Characterization Of Nanopipettes In High Ionic Strength Mediamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Characterization of Nanopipettes

et al. 2016

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“…During each experiment, the applied voltage always had a positive bias applied to the electrode underneath the membrane i.e. on the opposite to where the sample was placed, this orientation makes it easier to observe negatively charged beads 3 . We noted two distinct changes in the signal upon the binding of Thrombin-15 aptamer to the beads.…”

Section: Binding Of Biotinylated Dna To the Streptavidin Beadsmentioning

confidence: 99%

“…In recent years, developments in the field of diagnostics and biosensor technologies have been aided by the use of nanomaterials [1][2][3][4][5] . Synthesis and fabrication methods for nanoparticles in solution have evolved to the extent that particle size, shape, and composition can be easily modified.…”

Section: Introduction -mentioning

confidence: 99%

Monitoring Aptamer–Protein Interactions Using Tunable Resistive Pulse Sensing

Billinge

Broom²,

Platt

2013

Anal. Chem.

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-Aptamers are short single-stranded pieces of DNA or RNA capable of binding to analytes with specificity and high affinity. Due to their comparable selectivity, stability and cost, over the last two decades aptamers have started to challenge antibodies in their use on many technology platforms. The binding event often leads to changes in the aptamer's secondary and tertiary structure; monitoring such changes has led to the creation of many new analytical sensors. Here we demonstrate the use of a tunable resistive pulse sensing (TRPS) technology to monitor the interaction between several DNA aptamers and their target -thrombin. We immobilised the aptamers onto the surface of superparamagnetic beads, prior to their incubation with the thrombin protein. The protein binding to the aptamer caused a conformational change resulting in the shielding of the polyanion backbone; this was monitored by a change in the translocation time and pulse frequency of the particles traversing the pore. This signal was sensitive enough to allow the tagless detection of thrombin down to nanomolar levels. We further demonstrate the power of TRPS by performing real time detection and characterisation of the aptamer-target interaction and measuring the association rates of the thrombin protein to the aptamer sequences.

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Section: (A) Fabrication Of Glass Nanopipettesmentioning

confidence: 99%

Resistive-pulse and rectification sensing with glass and carbon nanopipettes

Wang

Mirkin

2017

Proc. R. Soc. A.

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Along with more prevalent solid-state nanopores, glass or quartz nanopipettes have found applications in resistive-pulse and rectification sensing. Their advantages include the ease of fabrication, small physical size and needle-like geometry, rendering them useful for local measurements in small spaces and delivery of nanoparticles/biomolecules. Carbon nanopipettes fabricated by depositing a thin carbon layer on the inner wall of a quartz pipette provide additional means for detecting electroactive species and fine-tuning the current rectification properties. In this paper, we discuss the fundamentals of resistivepulse sensing with nanopipettes and our recent studies of current rectification in carbon pipettes.

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Resistive-pulse measurements with nanopipettes: detection of Au nanoparticles and nanoparticle-bound anti-peanut IgY

Cited by 94 publications

References 55 publications

Characterization of Nanopipettes

Characterization of Nanopipettes

Monitoring Aptamer–Protein Interactions Using Tunable Resistive Pulse Sensing

Resistive-pulse and rectification sensing with glass and carbon nanopipettes

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