Resistive-Pulse Analysis of Nanoparticles

Luo, Long; German, Sean R.; Lan, Wen−How; Holden, Deric A.; Mega, Tony L.; White, Henry S.

doi:10.1146/annurev-anchem-071213-020107

Cited by 141 publications

(156 citation statements)

References 146 publications

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“…The shape of the ionic current blockades—a downward spike—was consistent with that produced by a spherical particle passing through a larger pore. 31 During the translocation process, the micelle was observed to deform slightly, recovering its spherical shape after each passage. Supporting Information Movie 6 illustrates a typical simulation trajectory.…”

Section: Molecular Dynamics Simulationsmentioning

confidence: 99%

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SDS-assisted protein transport through solid-state nanopores

et al. 2017

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Using nanopores for the single-molecule sequencing of proteins – similar to nanopore-based sequencing of DNA – faces multiple challenges, including unfolding of the complex tertiary structure of the proteins and enforcing their unidirectional translocation through nanopores. Here, we combine molecular dynamics (MD) simulations with single-molecule experiments to investigate the utility of SDS (Sodium Dodecyl Sulfate) to unfold proteins for solid-state nanopore translocation, while simultaneously endowing them with a stronger electrical charge. Our simulations and experiments probe that SDS-treated proteins show considerable loss of protein structure during the nanopore translocation. Moreover, SDS-treated proteins translocate through the nanopore in the direction prescribed by the electrophoretic force fue to the negative charge impaired by SDS. In summary, our results suggest that SDS causes protein unfolding while facilitating protein translocation in the direction of the electrophoretic force, both characteristics could bring great advantages for future protein sequencing applications using solid-state nanopores.

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Section: Molecular Dynamics Simulationsmentioning

confidence: 99%

“…This observation indicates that the amplitude of nanopore blockades produced by a compact object, such as a folded protein or an SDS micelle, depends on the nanopore size. 31 …”

Section: Molecular Dynamics Simulationsmentioning

confidence: 99%

SDS-assisted protein transport through solid-state nanopores

et al. 2017

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“…the half-width of the resistive-pulse) can be expected when such a particle enters a pipette with the radius a and angle θ? The extensive literature on mathematical modelling and numerical simulations of particle translocation through conical and cylindrical nanopores has recently been reviewed [54]. An approximate mathematic model developed for conical pores [55,56] was later adapted for a spherical particle translocating through a nanopipette [31] to calculate the shape and amplitude of the current pulse (figure 4).…”

Section: (B) Theorymentioning

confidence: 99%

Resistive-pulse and rectification sensing with glass and carbon nanopipettes

Wang

Mirkin

2017

Proc. R. Soc. A.

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Along with more prevalent solid-state nanopores, glass or quartz nanopipettes have found applications in resistive-pulse and rectification sensing. Their advantages include the ease of fabrication, small physical size and needle-like geometry, rendering them useful for local measurements in small spaces and delivery of nanoparticles/biomolecules. Carbon nanopipettes fabricated by depositing a thin carbon layer on the inner wall of a quartz pipette provide additional means for detecting electroactive species and fine-tuning the current rectification properties. In this paper, we discuss the fundamentals of resistivepulse sensing with nanopipettes and our recent studies of current rectification in carbon pipettes.

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“…Although Gershow et al 14 raised the possibility, only Sen et al 17 and Berge et al 15 Conical nanopores have unique advantages over rectangular nanochannels and cylindrical solid-state nanopores due to their ability to control nanoparticle dynamics, measure small 4 particles, and significantly improve the probability that a particle "captured" from one side of the pore will be "released" back to that side by a return translocation. [20][21][22][23] In this report, we employ a conical nanopore in an automated pressure-reversal system that allows controlled trapping, and repeated translocations, of individual particles based on automated electronic triggering of the particle motion using the translocation pulses. This approach permits multiple observations of single particles, thereby improving measurement resolution to sub-nanometer levels traditionally associated with ex-situ electron microscopy.…”

mentioning

confidence: 99%

Sizing Individual Au Nanoparticles in Solution with Sub-Nanometer Resolution

et al. 2015

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Resistive-pulse sensing has generated considerable interest as a technique for characterizing nanoparticle suspensions. The size, charge, and shape of individual particles can be estimated from features of the resistive pulse, but the technique suffers from an inherent variability due to the stochastic nature of particles translocating through a small orifice or channel. Here, we report a method, and associated automated instrumentation, that allows repeated pressure-driven translocation of individual particles back and forth across the orifice of a conical nanopore, greatly reducing uncertainty in particle size that results from streamline path distributions, particle diffusion, particle asphericity, and electronic noise. We demonstrate ∼0.3 nm resolution in measuring the size of nominally 30 and 60 nm radius Au nanoparticles of spherical geometry; Au nanoparticles in solution that differ by ∼1 nm in radius are readily distinguished. The repetitive translocation method also allows differentiating particles based on surface charge density, and provides insights into factors that determine the distribution of measured particle sizes.

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Resistive-Pulse Analysis of Nanoparticles

Cited by 141 publications

References 146 publications

SDS-assisted protein transport through solid-state nanopores

SDS-assisted protein transport through solid-state nanopores

Resistive-pulse and rectification sensing with glass and carbon nanopipettes

Sizing Individual Au Nanoparticles in Solution with Sub-Nanometer Resolution

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