Resistin down‐regulates insulin receptor expression, and modulates cell viability in rodent pancreatic beta‐cells

Brown, James E.; Onyango, David James; Dunmore, Simon

doi:10.1016/j.febslet.2007.06.031

Cited by 39 publications

(35 citation statements)

References 22 publications

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“…14 This observation may confirm that resistin contributes to insulin resistance in islets. In BRIN-BD11 b cell line resistin failed to affect insulin secretion at supraphysiologically high glucose (22 mM).…”

supporting

confidence: 72%

Resistin is produced by rat pancreatic islets and regulates insulin and glucagon in vitro secretion

Sassek

Pruszyńska‐Oszmałek

Kołodziejski

et al. 2016

Islets

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Resistin participates in the regulation of energy homeostasis, insulin resistance, and inflammation. The potential expression in pancreas, and modulation of the endocrine pancreas secretion by resistin is not well characterized, therefore, we examined it on several levels.We examined the localization of resistin in rat pancreatic islets by immunohistochemistry and immunofluorescence, and the potential presence of resistin mRNA by RT-PCR and protein by Western Blot in these structures. In addition, we studied the regulation of insulin and glucagon secretion by resistin in pancreatic INS-1E b-and InR-G9 a-cell lines as well as isolated rat pancreatic islets.We identified resistin immunoreactivity in the periphery of rat pancreatic islets and confirmed the expression of resistin at mRNA and protein level. Obtained data indicated that resistin is co-localized with glucagon in pancreatic a-cells. In addition, we found that in vitro resistin decreased insulin secretion from INS-1E cells and pancreatic islets at normal (6 mM) and high (24 mM) glucose concentrations, and also decreased glucagon secretion from G9 cells and pancreatic islets at 1 mM, whereas a stimulation of glucagon secretion was observed at 6 mM glucose. Our results suggest that resistin can modulate the secretion of insulin and glucagon from clonal b or a cells, and from pancreatic islets.

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supporting

confidence: 72%

Resistin is produced by rat pancreatic islets and regulates insulin and glucagon in vitro secretion

Sassek

Pruszyńska‐Oszmałek

Kołodziejski

et al. 2016

Islets

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“…This mechanism is consistent with gene targeting studies in mice that demonstrated proximal insulin signalling to be required to maintain adequate beta cell mass and function (Cantley et al 2007;Kulkarni et al 1999). Another study showed that 24-h resistin treatment of b-TC6 beta cells decreased insulin receptor mRNA levels, whereas treatment of BRIN-BD11 beta cells did not affect insulin secretion (Brown et al 2007). In studies of fixed human pancreata, resistin was shown to be expressed in human beta cells, but not alpha cells, and its expression increased with T2D (AlSalam et al 2011).…”

Section: Resistinsupporting

confidence: 50%

The control of insulin secretion by adipokines: current evidence for adipocyte-beta cell endocrine signalling in metabolic homeostasis

Cantley

2014

Mamm Genome

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Metabolic homeostasis is maintained by the coordinated action of multiple organ systems. Insulin secretion is often enhanced during obesity or insulin resistance to maintain glucose and lipid homeostasis, whereas a loss of insulin secretion is associated with type 2 diabetes. Adipocytes secrete hormones known as adipokines which act on multiple cell types to regulate metabolism. Many adipokines have been shown to influence beta cell function by enhancing or inhibiting insulin release or by influencing beta cell survival. Insulin, in turn, regulates lipolysis and promotes glucose uptake and lipid storage in adipocytes. As adipokine secretion and action is strongly influenced by obesity, this provides a potential route by which beta cell function is coordinated with adiposity, independently of alterations in blood glucose or lipid levels. In this review, I assess the evidence for the direct regulation of beta cell function by the adipokines leptin, adiponectin, extracellular nicotinamide phosphoribosyltransferase, apelin, resistin, retinol binding protein 4, fibroblast growth factor 21, nesfatin-1 and fatty acid binding protein 4. I summarise in vitro and in vivo data and discuss the influence of obesity and diabetes on circulating adipokine concentrations, along with the potential for influencing beta cell function in human physiology. Finally, I highlight future research questions that are likely to yield new insights into the exciting field of insulinotropic adipokines.

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“…However, Brown et al(2007) reported that incubation with 40 ng/ml resistin significantly decreased insulin receptor mRNA and protein expression, but did not affect insulin secretion in mice. Liu et al(2006) found that rat resistin concentrates in the cytoplasm around cell nucleus.…”

Section: Discussionmentioning

confidence: 99%

“…However, the mechanism of resistin in inducing insulin resistance remains poorly understood. It was reported that resistin down-regulated mRNA and protein expression of insulin receptor in rodent pancreatic beta-cells, but did not affect insulin secretion (Brown et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Resistin does not down-regulate the transcription of insulin receptor promoter

Qiao

Wang

et al. 2008

J. Zhejiang Univ. Sci. B

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Abstract:Objective: To detect the effect of resistin on the transcription of insulin receptor promoter. Methods: Luciferase reporter gene was fused downstream of human insulin receptor promoter and the enzymatic activity of luciferase was determined in the presence or absence of resistin. The resistin expressed with plasmid was stained with antibody against Myc tag which was in frame fused with resistin coding sequence, and then imaged with confocal microscopy. Results: The treatment of pIRP-LUC transfected cells with recombinant resistin did not result in significant difference in the enzymatic activity of luciferase compared to the untreated cells. Cell staining showed that green fluorescence could be observed in the cytoplasm, but not in the nucleus. Conclusion: The results suggest that the endogenous resistin may functionally locate in the cytoplasm, but does not enter the nucleus and not down-regulate the transcription of insulin receptor promoter.

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Resistin down‐regulates insulin receptor expression, and modulates cell viability in rodent pancreatic beta‐cells

Cited by 39 publications

References 22 publications

Resistin is produced by rat pancreatic islets and regulates insulin and glucagon in vitro secretion

Resistin is produced by rat pancreatic islets and regulates insulin and glucagon in vitro secretion

The control of insulin secretion by adipokines: current evidence for adipocyte-beta cell endocrine signalling in metabolic homeostasis

Resistin does not down-regulate the transcription of insulin receptor promoter

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