Resistin – 420 C/G polymorphism and serum resistin level in Iranian patients with gestational diabetes mellitus

Abstract

BackgroundResistin is a circulating adipokine with insulin-antagonizing effects. The aim of this study was to investigate the relationship between the single nucleotide polymorphism (SNP) -420C > G in the resistin gene with serum resistin levels, insulin resistance, and risk of gestational diabetes (GDM) in Iranian population.Method75 GDM patients and 70 healthy pregnant women were enrolled in this study. Genotyping for SNP- 420C > G in the resistin gene was performed by the polymerase chain reaction- restriction fragment length polymorphism (PCR-RFLP) method. Serum resistin and insulin were measured by immunoassay. Blood glucose levels and lipid profile were measured by enzymatic methods. Homeostasis model of assessment for insulin resistance (HOMA-IR) were calculated.ResultGG genotype and G allele of SNP-420C > G were more frequent in GDM patients compared to non-GDM subjects. Serum resistin level was similar in GDM and non-GDM patients. The serum levels of resistin in GDM and non-GDM women with GG genotype were similar to those with GC + CC genotype. Multivariate logistic regression analysis after adjusting for confounding factors showed a higher susceptibility to GDM in patients with GG genotype compared to subjects with GG + GT genotype (odds ratio = 4.59, 95% CI; 1.96-10.71, p = 0.00). Serum resistin level was correlated with serum triglyceride, total and low density lipoprotein (LDL) cholesterol (p < 0.05) in GDM patients. No significant association was found between serum resistin, insulin resistance, and SNP-420C > G.ConclusionThe SNP-420C/G of resistin gene is associated with genetic susceptibility to GDM in our population. Further studies are necessary to confirm the role of this polymorphism in pathogenesis of GDM and to explore potential mechanisms by which it modulates susceptibility to GDM.

Leptin (rs7799039) and solute carrier family 30 zinc transporter (rs13266634) polymorphisms in Euro-Brazilian pregnant women with gestational diabetes

“…This was as expected given that being overweight and having obesity (BMI >30 kg/m 2 ) are factors that contribute to the development of GDM (Murgia et al, 2008;Takhshid and Zare, 2015).…”

“…The BMI, age, and HbA1c parameters of pregnant women in the groups in question were similar to those in pregnant women in groups of similar studies (Huopio et al, 2013;Takhshid and Zare, 2015).…”

“…Insulin resistance is an important feature of gestational diabetes mellitus. Several adipokines, including leptin, are involved in the development of this resistance (Takhshid and Zare, 2015). The hormone leptin was first identified in adipose tissue and is known to be expressed in the placenta and fetal tissues (Vaskú et al, 2006).…”

The role of resistin gene polymorphisms in bladder cancer

“…A similar phenomenon was observed in endometrial cancer as higher serum resistin levels were associated with increased morbidity [18]. [21][22][23][24]. However, as far as we know, the link between RNTN SNPs and the incidence, development, and prognosis of bladder cancer have not been indicated by previous studies.…”

The Association of Resistin Gene –420c/G (Rs1862513) Single Nucleotide Polymorphism With Type Two Diabetes Mellitus in Iraqi Population

“…There are a number of genes identified as having an effect on developing the disease including resistin gene (RETN) [6]. the common variants single-nucleotide polymorphisms +299 G/T (rs3745367) and -420 C/G (rs1862513) studied in different populations to verify the susceptibility of individuals to T2DM [7,8,9,10,11,12,13]. Resistin is a circulating adipocytokine produced by white adipose which cause both insulin resistance [14]and metabolic syndrome [15].…”