Resistencia antimicrobiana en la infección urinaria por Escherichia coli adquirida en la comunidad: ¿Cuál antibiótico voy a usar?

Guajardo-Lara, Claudia Elena; González-Martínez, Pedro; Ayala-Gaytán, Juan Jacobo

doi:10.1590/s0036-36342009000200012

Cited by 18 publications

(10 citation statements)

References 13 publications

(17 reference statements)

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“…Interestingly, it was noticed that 7.3% (11/150) of the isolates were resistant to pefloxacin and 4% (6/150 isolates) were resistant to levofloxacin (Figure 4). These antibiotics are second and third-generation quinolones widely used in Mexico against intestinal and UTIs (Guajardo-Lara et al, 2009). Furthermore, 12 strains were FQ-resistant and presented a multi-resistant phenotype.…”

Section: Resultsmentioning

confidence: 99%

Presence of multi-drug resistant pathogenic Escherichia coli in the San Pedro River located in the State of Aguascalientes, Mexico

Castillo¹,

González²,

Garneau³

et al. 2013

Front. Microbiol.

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Contamination of surface waters in developing countries is a great concern. Treated and untreated wastewaters have been discharged into rivers and streams, leading to possible waterborne infection outbreaks and may represent a significant dissemination mechanism of antibiotic resistance genes. In this study, the water quality of San Pedro River, the main river and pluvial collector of the Aguascalientes State, Mexico was assessed. Thirty sample locations were tested throughout the River. The main physicochemical parameters of water were evaluated. Results showed high levels of fecal pollution as well as inorganic and organic matter abundant enough to support the heterotrophic growth of microorganisms. These results indicate poor water quality in samples from different locations. One hundred and fifty Escherichia coli were collected and screened by PCR for several virulence genes. Isolates were classified as either pathogenic (n = 91) or commensal (n = 59). The disc diffusion method was used to determine antimicrobial susceptibility to 13 antibiotics. Fifty-two percent of the isolates were resistant to at least one antimicrobial agent and 30.6% were multi-resistant. Eighteen E. coli strains were quinolone resistant of which 16 were multi-resistant. Plasmid-mediated quinolone resistance (PMQR) genes were detected in 12 isolates. Mutations at the Ser-83→Leu and/or Asp-87→Asn in the gyrA gene were detected as well as mutations at the Ser-80→Ile in parC. An E. coli microarray (Maxivirulence V 3.1) was used to characterize the virulence and antimicrobial resistance genes profiles of the fluoroquinolone-resistant isolates. Antimicrobial resistance genes such as blaTEM, sulI, sulII, dhfrIX, aph3 (strA), and tet (B) as well as integrons were found in fluoroquinolone (FQ) resistance E. coli strains. The presence of potential pathogenic E. coli and antibiotic resistance in San Pedro River such as FQ resistant E. coli could pose a potential threat to human and animal health.

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Section: Resultsmentioning

confidence: 99%

Presence of multi-drug resistant pathogenic Escherichia coli in the San Pedro River located in the State of Aguascalientes, Mexico

Castillo¹,

González²,

Garneau³

et al. 2013

Front. Microbiol.

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“…These results show that UPEC strains in Mexico City have become sufficiently resistant to treatment with penicillins, fluoroquinolones, and TMP/SMX, as to render their use contraindicated. Other national studies have also shown increasing uropathogenic resistance rates [18,19].…”

Section: Discussionmentioning

confidence: 97%

“…In addition, the prevalence of antimicrobial resistance in UPEC strains is an increasing concern in Mexico because there is growing resistance to the drugs of choice: ampicillin; cotrimoxazole, and ciprofloxacin. Furthermore, approximately 10% of isolates were resistant to ceftazidime, and three-fourths of these produced ESBLs [18,19]. In Mexico, the overall rate of antibiotic resistance is high, more so than in other comparable countries.…”

Section: Introductionmentioning

confidence: 99%

Drug resistance, serotypes, and phylogenetic groups among uropathogenic Escherichia coli including O25-ST131 in Mexico City

Molina-López¹,

Aparicio-Ozores²,

Ribas‐Aparicio³

et al. 2011

J Infect Dev Ctries

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Introduction: The increasing prevalence of uropathogenic Escherichia coli (UPEC) strains resistant to multiple antibiotics complicates the treatment of urinary tract infections (UTIs). This study aimed to analyze the antimicrobial resistance, serotypes, and phylogenetic groups among strains of E. coli isolated from outpatients with UTIs in Mexico City. Methodology: A total of 119 E. coli isolates were recovered from urine samples from outpatients with clinical diagnosis of uncomplicated UTIs from 2004 to 2007. The serotype was assessed by agglutination in microtiter plates; susceptibility to antimicrobials was determined by the disk diffusion method. Clone O25-ST131 and phylogenetic groups of E. coli strains were tested by methods based on PCR multiplex. Results: The predominant serotype was O25:H4 (21.2%). Resistance to antibiotics was ampicillin (83.7%); piperacillin (53.8%); the fluoroquinolone group (55.5-60.6%), and trimethoprim/sulfamethoxazole (TMP/SMX) (56.4%). Additionally, 36 (30.2%) isolates were multidrug-resistant and 13 of these 36 strains were identified as E. coli O25-ST131 clone by an allele-specific PCR-based assay. Phylogenetic analysis showed that 15 of 17 isolates with serotype O25:H4 belonged to group B2. Conclusions: This is the first report that establishes the presence in Mexico of the O25-ST131 clonal group of E. coli, which has been associated with multidrug-resistance and with high virulence potential. The spread of this clone in Mexico should be monitored closely. We found a correlation between serotype O25:H4 and multidrug resistance in UPEC strains. Our results indicate that the use of ampicillin, fluoroquinolones, and TMP/SMX should be reviewed when selecting empirical therapy for UTIs.

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“…Die Anwendung von Nitrofurantoin zur Behandlung akuter Infekte der ableitenden Harnwege erlebt derzeit eine Renaissance als Alternative zu TMP-SMX, denn die Resistenzraten von uropathogenen Keimen gegen Nitrofurantoin sind nach wie vor niedrig [14][15][16][17][18]. Nitrofurantoin könnte daher weiterhin ein geeigneter Wirkstoff zur Behandlung von Harnwegsinfekten sein, vor allem in Gegenden mit hoher beziehungsweise steigender Resistenz gegenüber TMP-SMX.…”

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“…Nitrofurantoin könnte daher weiterhin ein geeigneter Wirkstoff zur Behandlung von Harnwegsinfekten sein, vor allem in Gegenden mit hoher beziehungsweise steigender Resistenz gegenüber TMP-SMX. Dies ist beispielsweise in Mexiko City der Fall, einer Gegend mit Resistenzraten uropathogener Keime gegen TMP-SMX von über 50% [14,19]. Zur Untersuchung und Bestätigung der bakteriologischen und klinischen Wirksamkeit von Nitrofurantoin wurde in Mexiko City eine Studie bei Patienten mit akuten Infekten der unteren ableitenden Harnwege durchgeführt.…”

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Antimikrobielle und klinische Wirksamkeit von Nitrofurantoin in der Behandlung akuter Infekte der unteren ableitenden Harnwege bei Erwachsenen

Mendoza-Valdés

Rosete²,

Bueno³

et al. 2010

Med Klin

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Resistencia antimicrobiana en la infección urinaria por Escherichia coli adquirida en la comunidad: ¿Cuál antibiótico voy a usar?

Abstract: Resistance to trimethoprim-sulfamethoxazole and ciprofloxacin used for empiric treatment in community urinary infections is high, and there are few available treatment options.

Cited by 18 publications

References 13 publications

Presence of multi-drug resistant pathogenic Escherichia coli in the San Pedro River located in the State of Aguascalientes, Mexico

Presence of multi-drug resistant pathogenic Escherichia coli in the San Pedro River located in the State of Aguascalientes, Mexico

Drug resistance, serotypes, and phylogenetic groups among uropathogenic Escherichia coli including O25-ST131 in Mexico City

Antimikrobielle und klinische Wirksamkeit von Nitrofurantoin in der Behandlung akuter Infekte der unteren ableitenden Harnwege bei Erwachsenen

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