Resistant Body Formation inNeocallimastixSp., An Anaerobic Fungus from the Rumen of a Cow

A fast and reliable procedure for spore collection from anaerobic fungi: Application for RNA uptake and long-term storage of isolates

“…In addition, most previous reports did not conduct any viability measurements on the released spores. More importantly, the above approaches depend on the presence of free spores in the culture supernatant [7,8,[10][11][12]47]. Such dependency on the presence of free spores could be problematic in monocentric species (e.g.…”

“…AMT transformation, RNAi). Similarly, apart from microscopic-based observations [5][6][7][8][9][10][11][12], developmental biology studies seeking to understand the physiological, structural, regulatory, and gene expression patterns associated with various stages of development within the AGF complex life cycle have been extremely sparse.…”

Chytridiomycetous gut fungi, oft overlooked contributors to herbivore digestion

“…However, it must have been reestablished because it is always present in mature animals. The observation of resistant structures in faeces (Wubah et al 1991a) and the ability to isolate fungi from both faeces and saliva (see below) suggest that either "contaminated" feed or mother-daughter mouth contact is the most likely mechanism of acquiring the fungi in young animals.…”

“…these were resistant sporangia comparable to those known from aerobic chytrids. They failed to germinate these structures (Wubah et al 1991a) but it is highly likely that they simply did not find the probable specialized trigger needed. Similar structures were also found in both dry and moist faeces (Wubah et al 1991d), which shows that they are likely to be an important part of the life cycle in the wild.…”

“…Sexual reproduction of gut fungi has not been found, but resistant sporangia (Wubah et al 1991a) may represent such a stage because they are morphologically similar to the sexual reproductive structures found in the related aerobic chytridiomycetes.…”