Resistant and Susceptible Pinus thunbergii ParL. Show Highly Divergent Patterns of Differentially Expressed Genes during the Process of Infection by Bursaphelenchus xylophilus

Sun, Tingyu; Rahman, Mati Ur; Wu, Xiaoqin; Ye, Jianren

doi:10.3390/ijms241814376

Cited by 2 publications

(2 citation statements)

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“…28-40) was introduced from Japan in 2004 [85,86]. The susceptible P. thunbergii (mortality rates were all 100% after inoculation with PWN in the previous test) from Suqian City, China, was selected as the control [34].…”

Section: Plant Materials and Nematode Infectionsmentioning

confidence: 99%

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Pinus thunbergii Parl. Somatic Plants’ Resistance to Bursaphelenchus xylophilus Depends on Pathogen-Induced Differential Transcriptomic Responses

Sun,

Wang,

et al. 2024

IJMS

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Pinus thunbergii Parl. is an economically and medicinally important plant, as well as a world-renowned horticultural species of the Pinus genus. Pine wilt disease is a dangerous condition that affects P. thunbergii. However, understanding of the genetics underlying resistance to this disease is poor. Our findings reveal that P. thunbergii’s resistance mechanism is based on differential transcriptome responses generated by the early presence of the pathogen Bursaphelenchus xylophilus, also known as the pine wood nematode. A transcriptome analysis (RNA-seq) was performed to examine gene expression in shoot tissues from resistant and susceptible P. thunbergii trees. RNA samples were collected from the shoots of inoculated pines throughout the infection phases by the virulent Bursaphelenchus xylophilus AMA3 strain. The photosynthesis and plant–pathogen interaction pathways were significantly enriched in the first and third days after infection. Flavonoid biosynthesis was induced in response to late infestation (7 and 14 days post-infestation). Calmodulin, RBOH, HLC protein, RPS, PR1, and genes implicated in phytohormone crosstalk (e.g., SGT1, MYC2, PP2C, and ERF1) showed significant alterations between resistant and susceptible trees. Furthermore, salicylic acid was found to aid pine wood nematodes tolerate adverse conditions and boost reproduction, which may be significant for pine wood nematode colonization within pines. These findings provide new insights into how host defenses overcame pine wood nematode infection in the early stage, which could potentially contribute to the development of novel strategies for the control of pine wilt disease.

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Section: Plant Materials and Nematode Infectionsmentioning

confidence: 99%

“…The PWN used in this experiment was the highly virulent B. xylophilus strain AMA3 isolate. Nematode infection was conducted as described earlier by our group [34]. The average heights of the resistant and susceptible trees were 1.47 m and 1.51 m, respectively.…”

Section: Plant Materials and Nematode Infectionsmentioning

confidence: 99%