Resistance transferance of Amorimia septentrionalis poisoning in goats by ruminal transferance fluid from goats with induced resistance by the inoculation of sodium monofluoroacetate-degrading bacteria

Silva, Layze Cilmara Alves da; Pessoa, Danielle Aluska do Nascimento; Lopes, José R.G.; Silva, Leomyr Sângelo Alves da; Albuquerque, Laio Gomes de; Medeiros, Rosane M.T.; Garino, Felício; Riet-Correa, Franklin

doi:10.1590/0103-8478cr20141231

Cited by 5 publications

(3 citation statements)

References 15 publications

(26 reference statements)

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“…Transfer of FA degrading bacteria or the rumen fluid containing these bacteria to animals susceptible to FA also resulted in a higher resistance to intoxication by this compound. [102,103] The natural occurrence of FA degrading bacteria in the gastrointestinal system of ruminant animals has also been reported. [104] The protection of livestock animals from FA toxicity is of particular importance, given the significant economic losses caused by the ingestion of FA contaminated plant materials.…”

Section: Resistance Mechanisms To Fluoroacetatementioning

confidence: 98%

Natural production of fluorinated compounds and biotechnological prospects of the fluorinase enzyme

Carvalho

Oliveira

2017

Critical Reviews in Biotechnology

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Fluorinated compounds are finding increasing uses in several applications. They are employed in almost all areas of modern society. These compounds are all produced by chemical synthesis and their abundance highly contrasts with fluorinated molecules of natural origin. To date, only some plants and a handful of actinomycetes species are known to produce a small number of fluorinated compounds that include fluoroacetate (FA), some ω-fluorinated fatty acids, nucleocidin, 4-fluorothreonine (4-FT), and the more recently identified (2R3S4S)-5-fluoro-2,3,4-trihydroxypentanoic acid. This largely differs from other naturally produced halogenated compounds, which totals more than 5000. The mechanisms underlying biological fluorination have been uncovered after discovering the first actinomycete species, Streptomyces cattleya, that is capable of producing FA and 4-FT, and a fluorinase has been identified as the enzyme responsible for the formation of the C-F bond. The discovery of this enzyme has opened new perspectives for the biotechnological production of fluorinated compounds and many advancements have been achieved in its application mainly as a biocatalyst for the synthesis of [F]-labeled radiotracers for medical imaging. Natural fluorinated compounds may also be derived from abiogenic sources, such as volcanoes and rocks, though their concentrations and production mechanisms are not well known. This review provides an outlook of what is currently known about fluorinated compounds with natural origin. The paucity of these compounds and the biological mechanisms responsible for their production are addressed. Due to its relevance, special emphasis is given to the discovery, characterization and biotechnological potential of the unique fluorinase enzyme.

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Section: Resistance Mechanisms To Fluoroacetatementioning

confidence: 98%

Natural production of fluorinated compounds and biotechnological prospects of the fluorinase enzyme

Carvalho

Oliveira

2017

Critical Reviews in Biotechnology

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“…The goats that underwent transfaunation were later found to be more resistant to poisoning than the controls. Silva et al (2015) carried out rumen transfaunation from goats considered resistant to poisoning by A. septentrionalis, after oral administration of Ancylobacter dichloromethanicus and Pigmentiphaga kullae bacteria, to poisoning-sensitive goats, that is, animals that had never had contact with MFA-containing plants. After the transfaunation, A. septentrionalis was administered at a dose of 5g/kg to a group of goats, whereas the other goats received only the plant as controls.…”

Section: Use Of Rumen Transfaunation To Transfer Resistance To Poisoning By Mfa-containing Plantsmentioning

confidence: 99%

Techniques for prevention and control of poisoning by sodium monofluoroacetate (MFA)-containing plants in ruminants

2019

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Monofluoroacetate (MFA)-containing plants cause sudden death associated with exercise in ruminants, and are responsible for significant losses in Brazilian livestock, estimated at 500,000 bovine deaths annually. Most of the times, the control and treatment of this type of poisoning are not efficient, because disease evolution is superacute, usually causing the death of the animal. Due to the difficulty in controlling this intoxication, several studies have suggested alternatives to prevent it, mainly by making animals resistant to the MFA present in these plants or by avoiding their consumption. This literature review addresses the techniques used experimentally to control the poisoning of ruminants by plants containing MFA. The first studies carried out in Brazil demonstrated that goats and sheep that continuously receive non-toxic doses of plant containing MFA show greater resistance to poisoning than untreated animals, and that this resistance can be transmitted by ruminal fluid transfaunation, suggesting that poisoning occurs due to the presence of bacteria that hydrolyze MFA in the rumen. Based on this hypothesis, several MFA-hydrolyzing bacteria were isolated (Enterococcus faecalis, Bacillus sp., Paenibacillus sp., Burkholderia sp., Cupriavidus sp., Staphylococcus sp., Ancylobacter sp., Ralstonia sp., Stenotrophomonas sp., Pigmentiphaga kullae, and Ancylobacter dichloromethanicus). When some of these bacteria were administered intraruminally, they provided the animal with a different level of protection against poisoning. However, it was observed that protection is gradually lost when the bacterium administration is interrupted. Consequently, to obtain more efficient protection, these bacteria should be administered continuously, probably in the form of probiotics. In another assay, MFA was administered to sheep at non-toxic doses to test the hypothesis that this substance could induce the multiplication of bacteria that hydrolyze it in the rumen. There was no increase in resistance to poisoning after administration of MFA; however, no signs of poisoning were observed when animals received trifluoroacetate and no clinical signs were verified when they were challenged with toxic doses of MFA; in contrast, all control animals presented clinical signs. These results suggest that trifluoroacetate induces the proliferation of MFA-degrading bacteria, and can be used in intoxication prophylaxis. The conditioned food aversion technique, using lithium chloride, has been successfully used experimentally to prevent ruminants from ingesting plants that contain MFA. Another alternative tested was the spraying of Amorimia septentrionalis with the endophytic bacterium Herbaspirillum seropedicae, which degrades MFA, resulting in decreased concentration of this compound in the plants. In conclusion, several experimental techniques have been proved efficient in the control and prophylaxis of MFA-containing plant poisoning; however, none of these techniques are available commercially. Further experiments, mainly in the field, should be carried out to adapt some of these techniques to the conditions of extensive breeding in the numerous areas where MFA-containing plants occur.

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“…Control and prevention of this type of poisoning by eliminating the plant or by avoiding its consumption by animals is not always effective (Barbosa et al 2003). New alternatives are suggested to make animals resistant to poisoning, including: repeated administration, at alternate periods, of non-toxic doses of the plant (Duarte et al 2014); the transfer of ruminal fluid from ruminants inoculated with MFA-degrading bacteria that became resistant to intoxication to susceptible ruminants (Silva et al 2015); the administration of MFA-degrading bacteria isolated from the rumen of goats, soil and plants containing MFA (Pessoa et al 2015, Silva et al 2016, Pessoa et al 2018; and the use of lithium chloride as a conditioned aversion technique (Brito et al 2016). Among these alternatives is the use of bacteria capable of degrading the MFA present in the plant.…”

Section: Introductionmentioning

confidence: 99%

Herbaspirillum seropedicae as a degrading bacterium of monofluoroacetate: effects of its inoculation in goats by ingesting Amorimia septentrionalis and the concentrations of this compound in plants sprayed with the bacterium

et al. 2019

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Herbaspirillum seropedicae is a nitrogen-fixing bacterium capable of using toxic compounds as a source of carbon. Bacteria with this capacity can be used to make animals resistant to plant poisoning containing monofluoroacetate (MFA), such as Amorimia septentrionalis. The aim of this study was to evaluate if H. seropedicae is efficient in the degradation of MFA present in A. septentrionalis and if the inoculation of this bacterium in goats confers protection to A. septentrionalis intoxication. Two experiments were performed: in the first experiment 12 goats were divided into 2 groups. Goats in Group 1 were orally administered a solution containing the H. seropedicae bacterium for 10 days. From day 10 onwards, they received a daily dose of 5g/kg of A. septentrionalis with the bacteriauntil clinical signs of intoxication were observed. Group 2 goats received only the plant at the same dose, also until the observation of clinical signs of intoxication. The amount of MFA found in A. septentrionalis used in the experiment with goats was 1.6±0.058μg/mg. The total plant dose ingested by all goats in Group 1 was 80.83±12.81g/kg (129.33±20.50mg/kg MFA), which were significantly greater (p<0.05) than those of Group 2 goats (39.16±19.08g/kg plant and 62.66±30.53mg/kg MFA). Group 1 goats took an average of 16.16±2.56 days to develop clinical signs of intoxication, significantly longer (p=0.0012) than Group 2 goats (7.83±3.81 days). Two Group 2 goats died on the same day that they developed clinical signs of intoxication. At necropsy of these two animals, no significant changes were observed. In the second experiment, samples of A. septentrionalis were sprayed with a solution containing H. seropedicae. Before and eight days after spraying, the samples were pressed and dried for quantitation of MFA. The amount of MFA present in samples of A. septentrionalis 8 days after spraying with H. seropedicae was significantly lower (p=0.017) than that found prior to spraying. It can be concluded that administration of H. seropedicae in goats is capable of causing greater resistance to A. septentrionalis intoxication, and spraying the plant with this bacterium significantly reduces the amount of MFA in the plant.

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Resistance transferance of Amorimia septentrionalis poisoning in goats by ruminal transferance fluid from goats with induced resistance by the inoculation of sodium monofluoroacetate-degrading bacteria

Cited by 5 publications

References 15 publications

Natural production of fluorinated compounds and biotechnological prospects of the fluorinase enzyme

Natural production of fluorinated compounds and biotechnological prospects of the fluorinase enzyme

Techniques for prevention and control of poisoning by sodium monofluoroacetate (MFA)-containing plants in ruminants

Herbaspirillum seropedicae as a degrading bacterium of monofluoroacetate: effects of its inoculation in goats by ingesting Amorimia septentrionalis and the concentrations of this compound in plants sprayed with the bacterium

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