Resistance to widely-used disinfectants and heavy metals and cross resistance to antibiotics in Escherichia coli isolated from pigs, pork and pig carcass

Puangseree, Jiratchaya; Jeamsripong, Saharuetai; Prathan, Rangsiya; Pungpian, Chanika; Chuanchuen, Rungtip

doi:10.1016/j.foodcont.2021.107892

Cited by 26 publications

(22 citation statements)

References 43 publications

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“…In addition, S. aureus is notorious for its ability to acquire and/or develop resistance to antibiotics [5]. The acquisition mechanism of resistance in S. aureus is identical to that of other bacteria and this is mainly due to the enzymatic degradation of antibiotics, the modification of the target of the antibiotic, the change in membrane permeability and use of efflux pump, alternative metabolic pathways and interbacterial transmission of resistance genes through the horizontal transfer [8][9][10][11][12][13][14]. This attribute coupled with the high burden of S. aureus infections is a serious problem for treatment of staphylococcal infections [9].…”

Section: Introductionmentioning

confidence: 99%

Prolonged Exposure to Antimicrobials Induces Changes in Susceptibility to Antibiotics, Biofilm Formation and Pathogenicity in Staphylococcus aureus

Arsène

Podoprigora

Grigorievna

et al. 2021

JPRI

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Introduction: Frequent exposure to certain biocidal agents such as hypochlorous acid (HOCl), triclosan and benzalkonium chloride (BAC) has been reported to induce significant changes in Staphylococcus aureus. However, very few studies of this type have been conducted with conventional antimicrobials. Aim: The current investigation aimed to explore the phenotypic changes (susceptibility to antibiotics, biofilm formation and relative pathogenicity) that occur in S. aureus after recurrent exposure to antimicrobials. Methods: We compared the effects of long-term exposure to ampicillin, cefazoline, kanamycin and silver nanoparticles (AgNPs) on their susceptibility to antibiotics, biofilm formation, growth rate and pathogenicity in Staphylococcus aureus ATCC 6538. The minimum inhibitory concentrations (MIC) were determined using the microplate mircodilution method and the bacteria were exposed to increasing concentrations of each antimicrobial (MIC/2 to MIC) prepared in the BHIB for 8 days. The sensitivity of bacteria to antibiotics was assessed using the Kirby-Bauer disc diffusion method, the biofilm formation with crystal violet bacterial attachment assay and relative pathogenicity was assessed through a Galleria mellonella waxworm model. Results: The data in this investigation indicate that long-term exposure to antimicrobials may induce several changes in S. aureus. The exposure to ampicillin induced resistance to ceftazidime, tetracycline and ceftriaxone while the susceptibility to ceftazidime decreased in bacteria exposed to cefazolin and Kanamycin. Meanwhile, exposure to AgNPs induced some changes in susceptibility to trimethoprim and ceftazidime without causing resistance. Similarly, the strains exposed to ampicillin and kanamycin grew more rapidly and produced more biofilms than the control strains whereas the strains exposed to the AgNPs produced less biofilms. On G. melonella model, cefazolin seems to have attenuated the pathogenicity while the 3 other strains were more pathogenic than the controls. Conclusion: Long term exposure of S. aureus to antibiotics and AgNPs induces several changes in susceptibility to other antibiotics, growth rate, biofilm formation and pathogenicity; and these changes should be taken into account when choosing antibiotics for treatment of diseases caused by S. aureus.

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Section: Introductionmentioning

confidence: 99%

Prolonged Exposure to Antimicrobials Induces Changes in Susceptibility to Antibiotics, Biofilm Formation and Pathogenicity in Staphylococcus aureus

Arsène

Podoprigora

Grigorievna

et al. 2021

JPRI

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“…Persistent AMR and heavy metal resistant bacteria that have enhanced stress response characteristics and biochemical changes that compromise detection may be selected after exposure to biocides (Mourão et al., 2020; Rhouma et al., 2020). L. monocytogenes strains isolated from the poultry processing environment can also form biofilm, with benzalkonium chloride‐based cleaning programmes showing limited efficacy and exacerbating biofilm formation and AMR expression (Cadena et al., 2019; Rodríguez‐Campos et al., 2019; Puangseree et al., 2021).…”

Section: Assessmentmentioning

confidence: 99%

Role played by the environment in the emergence and spread of antimicrobial resistance (AMR) through the food chain

Koutsoumanis¹,

Allende²,

Álvarez‐Ordóñez³

et al. 2021

EFS2

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The role of food-producing environments in the emergence and spread of antimicrobial resistance (AMR) in EU plant-based food production, terrestrial animals (poultry, cattle and pigs) and aquaculture was assessed. Among the various sources and transmission routes identified, fertilisers of faecal origin, irrigation and surface water for plant-based food and water for aquaculture were considered of major importance. For terrestrial animal production, potential sources consist of feed, humans, water, air/dust, soil, wildlife, rodents, arthropods and equipment. Among those, evidence was found for introduction with feed and humans, for the other sources, the importance could not be assessed. Several ARB of highest priority for public health, such as carbapenem or extended-spectrum cephalosporin and/or fluoroquinolone-resistant Enterobacterales (including Salmonella enterica), fluoroquinolone-resistant Campylobacter spp., methicillin-resistant Staphylococcus aureus and glycopeptide-resistant Enterococcus faecium and E. faecalis were identified. Among highest priority ARGs bla CTX-M , bla VIM , bla NDM , bla OXA-48like , bla OXA-23 , mcr, armA, vanA, cfr and optrA were reported. These highest priority bacteria and genes were identified in different sources, at primary and post-harvest level, particularly faeces/manure, soil and water. For all sectors, reducing the occurrence of faecal microbial contamination of fertilisers, water, feed and the production environment and minimising persistence/recycling of ARB within animal production facilities is a priority. Proper implementation of good hygiene practices, biosecurity and food safety management systems is very important. Potential AMR-specific interventions are in the early stages of development. Many data gaps relating to sources and relevance of transmission routes, diversity of ARB and ARGs, effectiveness of mitigation measures were identified. Representative epidemiological and attribution studies on AMR and its effective control in food production environments at EU level, linked to One Health and environmental initiatives, are urgently required.

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“…It is then of no surprise that if animals raised for meat consumption harbour antibiotic-resistant microorganisms that those will find their ways into the meat to be consumed. Isolation of antibiotic-resistant microorganisms from various types of meat has been abundantly reported in the literature [105][106][107][108][109], including for pork [110,111].…”

Section: From Pig To Meatmentioning

confidence: 99%

“…As sampling goes down the processing line, detection of bacteria bearing resistance genes decline [120]. Part of the persistence in the meat plant environment may, at least in part, be explained by cross-resistance with sanitation products, particularly through multidrug efflux pumps [101,110,121]. In an early (1988) study, Mattila et al [122] investigated the transfer of antibiotic resistance in E. coli on the surface of meat at 20 and 4 • C. They concluded that the lack of transfer between donor and recipient cells was due to the sessile growth on the meat surface which prevented the cell contact necessary for the transfer of genetic materials.…”

Section: From Pig To Meatmentioning

confidence: 99%

Antibiotic Resistance: From Pig to Meat

et al. 2021

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Pork meat is in high demand worldwide and this is expected to increase. Pork is often raised in intensive conditions, which is conducive to the spread of infectious diseases. Vaccines, antibiotics, and other biosafety measures help mitigate the impact of infectious diseases. However, bacterial strains resistant to antibiotics are more and more frequently found in pig farms, animals, and the environment. It is now recognized that a holistic perspective is needed to sustainably fight antibiotic resistance, and that an integrated One Health approach is essential. With this in mind, this review tackles antibiotic resistance throughout the pork raising process, including their microbiome; many factors of their environment (agricultural workers, farms, rivers, etc.); and an overview of the impact of antibiotic resistance on pork meat, which is the end product available to consumers. Antibiotic resistance, while a natural process, is a public health concern. If we react, and act, collectively, it is expected to be, at least partially, reversible with judicious antibiotic usage and the development of innovative strategies and tools to foster animal health.

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Resistance to widely-used disinfectants and heavy metals and cross resistance to antibiotics in Escherichia coli isolated from pigs, pork and pig carcass

Cited by 26 publications

References 43 publications

Prolonged Exposure to Antimicrobials Induces Changes in Susceptibility to Antibiotics, Biofilm Formation and Pathogenicity in Staphylococcus aureus

Prolonged Exposure to Antimicrobials Induces Changes in Susceptibility to Antibiotics, Biofilm Formation and Pathogenicity in Staphylococcus aureus

Role played by the environment in the emergence and spread of antimicrobial resistance (AMR) through the food chain

Antibiotic Resistance: From Pig to Meat

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