Resistance to Rice Tungro Spherical Virus in Rice

Hibino, Hiroki; Daquioag, R. D.; Cabauatan, P. Q.; Dahal, G.

doi:10.1094/pd-72-0843

Cited by 19 publications

(3 citation statements)

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“…IR64, a widely grown cultivar across tropical Asia. IR64 is susceptible to RTSV (Hibino et al ., ), possessing a susceptible (S)‐type allele of eIF4G , whereas resistant cultivars able to suppress RTSV infection have the non‐S‐type allele of eIF4G with the nonsynonymous SNPs or deletion causing mutations at the Y 1059 V 1060 V 1061 residues (Lee et al ., ). Hence, we designed CRISPR/Cas9 reagents to target the sequences flanking this region of eIF4G with the aim to develop RTSV‐resistant varieties that could potentially be released as nongenetically modified plants to the market whether/when proper regulatory policies are set in place.…”

Section: Introductionmentioning

confidence: 99%

Novel alleles of rice eIF4G generated by CRISPR/Cas9‐targeted mutagenesis confer resistance to Rice tungro spherical virus

Macovei

Sevilla

Cantos

et al. 2018

Plant Biotechnology Journal

323

146

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SummaryRice tungro disease (RTD) is a serious constraint in rice production across tropical Asia. RTD is caused by the interaction between Rice tungro spherical virus (RTSV) and Rice tungro bacilliform virus. RTSV resistance found in traditional cultivars has contributed to a reduction in the incidence of RTD in the field. Natural RTSV resistance is a recessive trait controlled by the translation initiation factor 4 gamma gene (eIF4G). The Y1059V1060V1061 residues of eIF4G are known to be associated with the reactions to RTSV. To develop new sources of resistance to RTD, mutations in eIF4G were generated using the CRISPR/Cas9 system in the RTSV‐susceptible variety IR64, widely grown across tropical Asia. The mutation rates ranged from 36.0% to 86.6%, depending on the target site, and the mutations were successfully transmitted to the next generations. Among various mutated eIF4G alleles examined, only those resulting in in‐frame mutations in SVLFPNLAGKS residues (mainly NL), adjacent to the YVV residues, conferred resistance. Furthermore, our data suggest that eIF4G is essential for normal development, as alleles resulting in truncated eIF4G could not be maintained in homozygous state. The final products with RTSV resistance and enhanced yield under glasshouse conditions were found to no longer contain the Cas9 sequence. Hence, the RTSV‐resistant plants with the novel eIF4G alleles represent a valuable material to develop more diverse RTSV‐resistant varieties.

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Section: Introductionmentioning

confidence: 99%

Novel alleles of rice eIF4G generated by CRISPR/Cas9‐targeted mutagenesis confer resistance to Rice tungro spherical virus

Macovei

Sevilla

Cantos

et al. 2018

Plant Biotechnology Journal

323

146

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“…• It is becoming increasingly clear that IPM is an information-intensive approach and multiple tactics have to be employed to disseminate IPM globally, the CRSP has disseminated such information through the print media and electronic media as well as workshops and field days. Osada and Fucikovsky, 1986;Chavez et al, 1988;Hibino et al, 1988;Efron et al, 1989;Dahal et al, 1990;Dixon et al, 1992;Vuylsteke et al, 1993;Mahungu et al, 1994;Bandyopadhyay et al, 1998;Huet et al, 1999;Legg and Thresh, 2000;Bellotti and Arias, 2001;Morales, 2001), biological control of alien invasive species (e.g. Herren and Neuenschwander, 1991;Neuenschwander et al, 1994;van Thielen et al, 1994;Yaninek et al, 1998;Neuenschwander, 2001), substitution of inorganic pesticides with biopesticides (Lomer et al, 2001), and development of holistic approaches (e.g.…”

Section: Lessons Learnedmentioning

confidence: 99%

Integrated pest management in the global arena

2003

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This book presents experiences and successful case studies of integrated pest management (IPM) from developed and developing countries and from major international centres and programmes. It contains 39 chapters by many contributors addressing themes such as: emerging issues in IPM, including biotechnology, pesticide policies and socioeconomic considerations (8 chapters); country experiences from Africa, Asia, North and South America, Europe, Australia and New Zealand (20 chapters); and regional and international experiences, including those of FAO, USAID, ICIPE, CIRAD, the World Bank and CGIAR Systemwide IPM Program (9 chapters). This book will be of significant interest to those working in the areas of crop protection, entomology and pest management.

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“…Thus, previous views on the apparent durability of resistance to N. virescens (Khush 1984) have been changed and it is now accepted that populations are likely to adapt to resistant varieties within a few years of their general release (Ruangsook & Khush 1987). This explains the current interest in mechanisms of resistance, gene deployment and breeding strategies to provide varieties with more effective and durable forms of resistance, not only to the vector but also to the tungro viruses (Hibino et al 1988). …”

Section: Rice Tungro Diseasementioning

confidence: 99%

The ecology of tropical plant viruses

Thresh

1991

Plant Pathology

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Resistance to Rice Tungro Spherical Virus in Rice

Cited by 19 publications

References 0 publications

Novel alleles of rice eIF4G generated by CRISPR/Cas9‐targeted mutagenesis confer resistance to Rice tungro spherical virus

Novel alleles of rice eIF4G generated by CRISPR/Cas9‐targeted mutagenesis confer resistance to Rice tungro spherical virus

Integrated pest management in the global arena

The ecology of tropical plant viruses

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