Resistance to reinfection in mice as a vaccine model for giardiasis

Li, Erqiu; Liu, Mingqiu; Singer, Steven M.

doi:10.4161/hv.29116

Cited by 11 publications

(7 citation statements)

References 36 publications

(40 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Symptoms of giardiasis are much less severe in regions of endemicity than of nonendemicity, suggesting gradual build-up of immunity (18). Secretory IgA (19,20), intestinal hypermotility (21,22), and CD4 T cells (23) are important in the host defense, but other effectors have been proposed (24).…”

mentioning

confidence: 99%

Identification of Conserved Candidate Vaccine Antigens in the Surface Proteome of Giardia lamblia

et al. 2019

View full text Add to dashboard Cite

Giardia lamblia, one of the most common protozoal infections of the human intestine, is an important worldwide cause of diarrheal disease, malabsorption, malnutrition, delayed cognitive development in children, and protracted postinfectious syndromes. Despite its medical importance, no human vaccine is available against giardiasis. A crude veterinary vaccine has been developed, and experimental vaccines based on expression of multiple variant-specific surface proteins have been reported, but poorly defined vaccine components and excessive antigen variability are problematic for pharmaceutical vaccine production. To expand the repertoire of antigen candidates for vaccines, we reasoned that surface proteins may provide an enriched source of such antigens since key host effectors, such as secretory IgA, can directly bind to such antigens in the intestinal lumen and interfere with epithelial attachment. Here, we have applied a proteomics approach to identify 23 novel surface antigens of G. lamblia that show Ͼ90% amino acid sequence identity between the two humanpathogenic genetic assemblages (A and B) of the parasite. Surface localization of a representative subset of these proteins was confirmed by immunostaining. Four selected proteins, uridine phosphorylase-like protein-1, protein 21.1 (GL50803_27925), ␣1-giardin, and ␣11-giardin, were subsequently produced in recombinant form and shown to be immunogenic in mice and G. lamblia-infected humans and confer protection against G. lamblia infection upon intranasal immunization in rodent models of giardiasis. These results demonstrate that identification of conserved surface antigens provides a powerful approach for overcoming a key rate-limiting step in the design and construction of an effective vaccine against giardiasis.

show abstract

mentioning

confidence: 99%

Identification of Conserved Candidate Vaccine Antigens in the Surface Proteome of Giardia lamblia

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Research, Society and Development, v. 10, n. 10, e493101019250, 2021 (CC BY 4.0) | ISSN 2525-3409 | DOI: http://dx.doi.org/10. 33448/rsd-v10i10.19250 Infection with G. duodenalis in C57BL/6 mice was also reported when 10 6 trophozoites were administered by gavage (Li et al, 2014), or when these animals had concomitant infection with other parasite (Von-Allmen et al, 2006). Some authors report that cysts of G. duodenalis of assemblages A and B are not able to naturally infect mice and rats (Lebbad et al, 2010;Zhao, 2015), but the inoculation of trophozoites can lead to infection (Lemée et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

Male Swiss mice (Mus musculus) as a most suitable experimental model for the study of Giardia duodenalis BIV

Romera

Bezagio

Costa-Ferreira

et al. 2021

RSD

View full text Add to dashboard Cite

In this study, we proposed to verify the most suitable murine experimental model for studying human giardiasis. In total 150 animals were used. Fifty mice (Mus musculus) from each lineage (Swiss, Balb/c and C57BL/6), 25 females and 25 males, were divided into 5 groups with 5 animals each, according to the lineage/sex. Three groups were infected with 104 cysts of Giardia duodenalis of assemblage BIV and 2 negative control groups. The animals were followed and evaluated for 15 days after receiving the inoculum. The clinical parameters evaluated were body weight, water and feed intake, excretion, appearance of fur and feces, elimination of Giardia spp cysts and behavioral assessment. The clinical parameters of the groups infected with G. duodenalis were compared with the non-infected groups within their own lineage/sex. In the 15 days of monitoring, only the male Swiss mice presented differences in these parameters. The infected animals consumed more feed, water and eliminated more excreta than the non-infected group. There was no difference in the general average of the weight of the animals or in the behavioral assessment in any group. Only the infected male Swiss mice eliminated G. duodenalis cysts in the feces, which was confirmed by the molecular diagnosis and by observing the presence of trophozoites in the intestinal mucosa. The results demonstrate that the most suitable animal model for the study of human giardiasis is the male Swiss mice, since it was the only one capable of developing infection by G. duodenalis cysts.

show abstract

“…Splenocytes from Giardia ‐infected mice produced detectable levels of cytokines such as IL‐4, IL‐6, IFN‐γ and TNF‐α in response to low dose of the parasite extracts (1 μg/mL). While regulatory cytokines such as IL‐10 production were observed with greater doses of the extract (10 or 100 μg/mL) . Therefore, we used high dose of Giardia cyst extract (0.1 mg/mL) to allow the production of regulatory cytokines to prevent excessive tissue destruction and death of the host.…”

Section: Discussionmentioning

confidence: 99%

“…60 Oral administration of cyst wall proteins (CWP) mimics the im- extract (10 or 100 μg/mL). 62 Therefore, we used high dose of Giardia cyst extract (0.1 mg/mL) to allow the production of regulatory cytokines to prevent excessive tissue destruction and death of the host.…”

Section: Early In Toxoplasma Infection Ifnγ Is T-cell Independent Anmentioning

confidence: 99%

Immunological interaction between Giardia cyst extract and experimental toxoplasmosis

Ashour

Saad

Dawood

et al. 2017

Parasite Immunology

View full text Add to dashboard Cite

SummaryToxoplasmosis is mostly associated with other intestinal parasitic infections especially Giardia due to shared mode of peroral infection. Toxoplasma and Giardia induce a strong T-helper 1-immune response. Our aim was to induce a protective immune response that results in significant impact on intestinal and extra-intestinal phases of Toxoplasma infection. This study was conducted in experimental animals and assessment of Giardia cyst extract effect on Toxoplasma infection was investigated by histopathological examination of small intestine and brain, Toxoplasma cyst count and iNOS staining of the brain, measurement of IFN-γ and TGF-β in intestinal tissues. Results showed that the brain Toxoplasma cyst number was decreased in mice infected with Toxoplasma then received Giardia cyst extract as compared to mice infected with Toxoplasma only. This effect was produced because Giardia cyst extract augmented the immune response to Toxoplasma infection as evidenced by severe inflammatory reaction in the intestinal and brain tissues, increased levels of IFN-γ and TGF-β in intestinal tissues and strong iNOS staining of the brain. In conclusion, Giardia cyst extract generated a protective response against T. gondii infection. Therefore, Giardia antigen will be a suitable candidate for further researches as an immunomodulatory agent against Toxoplasma infection. K E Y W O R D SGiardia, IFN-γ, iNOS, TGF-β, Toxoplasma

show abstract

Resistance to reinfection in mice as a vaccine model for giardiasis

Cited by 11 publications

References 36 publications

Identification of Conserved Candidate Vaccine Antigens in the Surface Proteome of Giardia lamblia

Identification of Conserved Candidate Vaccine Antigens in the Surface Proteome of Giardia lamblia

Male Swiss mice (Mus musculus) as a most suitable experimental model for the study of Giardia duodenalis BIV

Immunological interaction between Giardia cyst extract and experimental toxoplasmosis

Contact Info

Product

Resources

About