Resistance to pathogens in terpene down-regulated orange fruits inversely correlates with the accumulation of D-limonene in peel oil glands

Rodríguez, Ana; Shimada, Takehiko; Cervera, Magdalena; Redondo, Ana; Alquézar, Berta; Rodrigo, María Jesús; Zacarı́as, Lorenzo; Palou, Lluı́s; López, Marı́a M.; Peña, Leandro

doi:10.1080/15592324.2015.1028704

Cited by 12 publications

(8 citation statements)

References 19 publications

(18 reference statements)

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“…Different citrus genotypes, including oranges, mandarins, lemons, citrons, and grapefruits, also emit high levels of D-limonene and linalool from flowers (Azam et al, 2013b ) and leaves (Azam et al, 2013a ). Additionally, both compounds have been reported as involved in citrus resistance to different pest and pathogens (Ben-Yehoshua et al, 2008 ; Rodríguez et al, 2011 , 2014 , 2015 ; Shimada et al, 2014 ; Rodrigues Marques et al, 2015 ). The existence of different genes with a common origin encoding redundant proteins could indicate that diversification, in addition to sequence level, has occurred at the level of gene expression patterns.…”

Section: Discussionmentioning

confidence: 99%

“…In rough lemon, monoterpenes emitted from leaves increase as response to wounding, and microbial infection (Yamasaki et al, 2007 ; Shishido et al, 2012 ), and the expression level of some terpene synthase (TPS) genes are increased by microbial attack (Shishido et al, 2012 ; Shimada et al, 2014 ). In citrus peel, high D-limonene content has been related with the attraction of a citrus pest (the medfly Ceratitis capitata ) and the infection by specialized citrus pathogens (Rodríguez et al, 2011 , 2015 ).…”

Section: Introductionmentioning

confidence: 99%

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Genomic Analysis of Terpene Synthase Family and Functional Characterization of Seven Sesquiterpene Synthases from Citrus sinensis

et al. 2017

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Citrus aroma and flavor, chief traits of fruit quality, are derived from their high content in essential oils of most plant tissues, including leaves, stems, flowers, and fruits. Accumulated in secretory cavities, most components of these oils are volatile terpenes. They contribute to defense against herbivores and pathogens, and perhaps also protect tissues against abiotic stress. In spite of their importance, our understanding of the physiological, biochemical, and genetic regulation of citrus terpene volatiles is still limited. The availability of the sweet orange (Citrus sinensis L. Osbeck) genome sequence allowed us to characterize for the first time the terpene synthase (TPS) family in a citrus type. CsTPS is one of the largest angiosperm TPS families characterized so far, formed by 95 loci from which just 55 encode for putative functional TPSs. All TPS angiosperm families, TPS-a, TPS-b, TPS-c, TPS-e/f, and TPS-g were represented in the sweet orange genome, with 28, 18, 2, 2, and 5 putative full length genes each. Additionally, sweet orange β-farnesene synthase, (Z)-β-cubebene/α-copaene synthase, two β-caryophyllene synthases, and three multiproduct enzymes yielding β-cadinene/α-copaene, β-elemene, and β-cadinene/ledene/allo-aromandendrene as major products were identified, and functionally characterized via in vivo recombinant Escherichia coli assays.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Genomic Analysis of Terpene Synthase Family and Functional Characterization of Seven Sesquiterpene Synthases from Citrus sinensis

et al. 2017

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“…These results may indicate that the damage induced at 12 ºC is not sufficient to increase limonene emission or that the emission of limonene may be a specific cold-induced response. This monoterpene has been demonstrated to be crucial in the citrus fruit response to different biotic stresses since the resistance to different pathogens or pests attraction (bacteria, mold and fruit fly) is inversely related to the accumulation of limonene in the oil gland of sweet orange peel (Rodríguez et al, 2015).…”

Section: Effect Of Temperature Storage (2 and 12 ºC) On Volatile Emismentioning

confidence: 99%

“…The response to pathogen attack, as example, drastically modify the volatile profile emission in plants which play key roles in the attraction of natural predators and in the induction of cellular defense mechanisms to cope with pathogenic infection (Rodríguez, Alquézar, & Peña, 2013). Transgenic sweet orange plants with a reduced expression of limonene synthase resulted in fruit with lower limonene but higher content of certain monoterpene alcohols, and showed a higher resistance to pathogens, suggesting a key role of these compounds in citrus fruit interaction with the environment (Rodríguez et al, 2015(Rodríguez et al, , 2018. Volatile profile in citrus fruit notably varies during fruit ripening, rootstock and growing conditions (Ortuño et al, 1995) as well as with postharvest practices (curing, degreening and waxing), storage conditions (Biolatto et al, 2005;Obenland, Collin, Sievert & Arpaia, 2013;Sdiri, Rambla, Besada, Granell, & Salvador, 2017;Tietel, Lewinsohn, Fallik, & Porat, 2012;Tietel, Plotto, Fallik, Lewinsohn, & Porat, 2011) and physiological disorders (Xie et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Influence of the storage temperature on volatile emission, carotenoid content and chilling injury development in Star Ruby red grapefruit

et al. 2019

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Grapefruits are sensitive to develop chilling injury (CI) on the peel upon postharvest storage at low temperature. We investigated the influence of the storage at 2 and 12 ºC on CI, carotenoids, and emission of volatiles by intact fruit. CI symptoms at 12 ºC were restricted to green fruit peel sectors but at 2 ºC the CI severity was higher and distributed through the whole fruit surface. Fruit peel coloration and carotenes content increased at 12 ºC whereas experienced minor changes at 2 ºC. At 2 ºC the emission of total volatiles and specific monoterpenes, mainly limonene, but also linalool and terpineol was enhanced, while storage at 12 ºC resulted in higher emission and diversity of cyclic sesquiterpenes and aliphatic esters. Results indicate a selective emission of volatiles by intact red grapefruit that appears to be a specific response to the storage temperature or to the cold-induced damage.

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“…To investigate the role of limonene in pathogen growth, the citrus limonene synthase gene (CitMTSE1) was down-regulated by an antisense approach (Rodríguez et al 2011). The down-regulation of limonene synthase, which caused a sharp reduction in limonene content in the transgenic plants, also led to a marked resistance against X. citri in the fruit peel (Rodríguez et al 2011(Rodríguez et al , 2015. In these studies, however, resistance to X. citri in infected leaves was not reported.…”

Section: Defense-related Genesmentioning

confidence: 99%

Xanthomonas citri subsp. citri: host interaction and control strategies

Martins

Andrade

Benedetti

et al. 2020

Trop. plant pathol.

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Citrus is one of the most ancient fruit crops cultivated in the world. For many countries, citrus production is an important source of revenues. However, despite the richness of citrus production worldwide, fruit yields are constantly threatened by diseases that cause serious economic and social impacts to growers and consumers. One such example is citrus canker, a disease that affects all commercial citrus varieties and for which there is no cure. Currently, control measures for citrus canker are restricted to the application of copper-based compounds and the elimination of infected trees to prevent pathogen spreading in the field. However, new alternatives for the control of this disease are being developed, spanning through transgenic plants to innovative chemicals and biological control. New genome editing approaches and a repertoire of plant defense-related genes have been exploited to produce citrus lineages more tolerant to X. citri. In addition, a series of new molecules have been tested to not only inhibit X. citri growth but to also boost the plant immune system to suppress disease progression. In this review, we provide the state of art of all citrus canker control measures in use today, highlighting their utility and drawbacks and commenting on novel strategies to better control this important citrus disease.

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Resistance to pathogens in terpene down-regulated orange fruits inversely correlates with the accumulation of D-limonene in peel oil glands

Cited by 12 publications

References 19 publications

Genomic Analysis of Terpene Synthase Family and Functional Characterization of Seven Sesquiterpene Synthases from Citrus sinensis

Genomic Analysis of Terpene Synthase Family and Functional Characterization of Seven Sesquiterpene Synthases from Citrus sinensis

Influence of the storage temperature on volatile emission, carotenoid content and chilling injury development in Star Ruby red grapefruit

Xanthomonas citri subsp. citri: host interaction and control strategies

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