Resistance to Meloidogyne paranaensis in wild Coffea arabica

Abstract

Nine accessions of wild Coffea arabica from Ethiopia were evaluated for resistance to Meloidogyne paranaensis. Two wellcharacterized susceptible and resistant cultivars were used as comparative controls. The experiments were conducted in a growth chamber using a clonal population of M. paranaensis (esterase phenotype P1) originating from Brazil. Resistance and susceptibility to the nematode were evaluated using the number of nematodes (eggs and J2) per plant, number of nematodes per gram of root and the reproduction factor (RF). All wild coffee accessions expressed a resistance response to M. paranaensis similar to that of the resistant control Nemaya (RF < 1.0). These results provide coffee breeders with material whose resistance can be transferred into commercial cultivars. Keywords: coffee, root-knot nematode, pathogenicity. RESUMO Avaliação da resistência de cafeeiros silvestres (Coffea arabica) a Meloidogyne paranaensisForam avaliados quanto à resistência a Meloidogyne paranaensis, nove acessos de cafeeiros silvestres incluindo dois cultivares bem caracterizados como testemunhas de suscetibilidade e resistência. Os experimentos foram realizados sob condições controladas em câmara de crescimento, utilizando uma população clonal de M. paranaensis (fenótipo de esterase P1), proveniente do Brasil. A resistência e a suscetibilidade ao nematóide foram avaliadas com base no número total de nematóides por planta (ovos + J2) e por grama de raiz e no fator de reprodução (FR). Todos os acessos mostraram resposta à infecção por M. paranaensis similar à da testemunha resistente (FR < 1,0). Com esses resultados, novos materiais, cuja resistência pode ser transferida aos cultivares comerciais, ficam disponíveis para os fitomelhoristas. Palavras-chave: café, nematóide das galhas, patogenicidade.

Coffea arabica lines with resistance to nematode Meloidogyne paranaensis derived from crossings with IPR 100

“…In the present study, statistical difference between the fresh weight of roots of genotypes it was observed (Table 1) and resistant genotypes tended to present a more developed root system. Boisseau et al (2009) reported that it was important to use the Nematodes.g -1 variable in addition to the total number of nematodes extracted per plant, since the coffee plant tested for M. paranaensis presented statistical differences in the weight of roots.…”

“…Plant resistance has been considered as one of the main nematode management strategies, especially for sedentary endoparasites, such as those from the Meloidogyne genus, which have a specialized interaction with their hosts (Roberts, 2002). Resistance to M. paranaensis has been found in Coffea canephora Pierre ex Froehner (Sera et al, 2006;Gonçalves and Silvarolla, 2007;Andreazi et al, 2015a), C. congensis Froehner (Gonçalves et al, 1988) and wild C. arabica L. accessions from Ethiopia Boisseau et al, 2009). Arabica coffee carrying C. canephora genes, such as "Icatu" and its derivatives (Gonçalves and Silvarolla, 2007;Andreazi et al, 2015b;Shigueoka et al, 2016a) and Híbrido de Timor derivatives (Salgado et al, 2014;Shigueoka et al, 2016b) also showed resistance to this nematode.…”

“…To assess the resistance to nematodes in Coffea spp., several parameters are used such as gall index (Gonçalves and Pereira, 1998;Noir et al, 2003;Muniz et al, 2009), egg mass index Ferraz, 1987, Muniz et al, 2009), reproduction factor (Salgado et al, 2005;Boisseau et al, 2009;Muniz et al, 2009), and number of nematodes per gram of roots (Gonçalves and Ferraz, 1987;Salgado et al, 2005;Boisseau et al, 2009). Some studies used the reduction in reproduction factor (Gonçalves and Pereira, 1998;Salgado et al, 2005;Andreazi et al, 2015) and the host susceptibility index (Gonçalves and Ferraz, 1987;Andreazi et al, 2015) to classify the resistance level of coffee plants.…”

A high-throughput method for early screening of coffee (Coffea spp.) genotypes for resistance to root-knot nematodes (Meloidogyne spp.)

“…A clonal population of M. paranaensis was established from a single egg mass of nematodes originally collected from coffee plants in Apucarana (Paraná State, Brazil) and then maintained in culture by Embrapa Recursos Genéticos e Biotecnologia (Boisseau et al 2009). The population was maintained in a confined greenhouse in Montpellier on cv.…”

“…The resistant accessions of species other than C. arabica can be efficiently used as rootstocks (Bertrand et al 2000b), constituting a cheap alternative to introgressive breeding (Lashermes et al 2000;Bertrand et al 2001). Sources of resistance to M. arabicida López, M. arenaria (Neal) Chitwood and M. paranaensis have been identified in both cultivated species (Bertrand et al 2000b(Bertrand et al , 2002Anzueto et al 2001;Hernández et al 2004;Boisseau et al 2009). Against M. exigua and M. incognita (Kofoid and White) Chitwood, resistant accessions have been detected only in C. canephora and progenies of interspecific hybrids (C. arabica x C. canephora) (Curi et al 1970;Gonçalves and Ferraz 1987;Carneiro 1995;Bertrand et al 1997;Gonçalves and Pereira 1998;Silvarolla et al 1998).…”