Resistance to Iron Deficiency of Five Peach Rootstocks

Stylinanides, D.C.; Tsipourides, C.Gr.; Michailides, Z.

doi:10.17660/actahortic.1989.254.28

Cited by 9 publications

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“…3.2.2. Effect of BAP with or without IBA on Average number of shoots per explant results for the average number of shoots per explant of the Okinawa, Nemared, and Garnem peach rootstocks as affected by the plant growth regulators are shown in Table (7) and Figs. (5g, h, i).…”

Section: Effect Of Bap With or Without Iba On Average Shoot Multiplic...mentioning

confidence: 99%

Comprehensive Study on In vitro Propagation of Some Imported Peach Rootstocksa. In vitro Explant Surface Sterilization and Bud Proliferation

Eliwa

2023

Preprint

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This study was conducted in the Laboratory of Tissue culture, Horticulture Department, Faculty of Agriculture, Damietta University, Egypt. The objective of this study was to establish a micropropagation protocol suitable for three imported peach rootstocks {Okinawa (P. persica), Nemared (P. persica × P. davidiana) × P. persica) and Garnem (P. dulcis × P. persica)} In vitro. According to the results, soaking the explants in sodium hypochlorite (NaOCl) at 20% for 15 min achieved the highest value of responsive (82.81%) and Survived (96.61%) with minimum value of dead (3.14%) and contaminated (0.24%). Garnem genotype explants had achieved the best responsive (89.12%) and Survived (90.62%) with minimum dead (0.00%) and maximum contaminated (9.37%). The shoot tips showed the highest responsive, Survived and dead% (100, 87.40 and12.59% respectively) and minimum significant contaminated (0.00%). Also, the explants differed greatly in their percentages of responsive, survived, dead and contaminated at the different collected dates. The 6-benzylaminopurine (BAP) concentrations used (3 to 5.0 mg/L-1) demonstrated a similar behavior in terms of in vitro proliferation, with rate of 3.77 to 6.11, 4.33 to 8.88 and 3.33 to 7.44 shoots per explant for Okinawa, Nemared and Garnem peach rootstocks respectively, indicating that the number of shoots is genotype dependent. Hence, 5.0 mg/l BAP in combination with 0.2 mg/L-1 IBA is recommended for shoot proliferation of peach rootstocks since this combination treatment produced the higher significant values of average shoot proliferation % (96.29%) as well as number of shoot per explant (7.48) and average Leaves number/explant (16.33) than the other treatments.

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Section: Effect Of Bap With or Without Iba On Average Shoot Multiplic...mentioning

confidence: 99%

Comprehensive Study on In vitro Propagation of Some Imported Peach Rootstocksa. In vitro Explant Surface Sterilization and Bud Proliferation

Eliwa

2023

Preprint

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“…Garnem, Nemaguard, Nemared, and Okinawa as tissue culture seedlings or seeds from the USA, Italy, Spain, and France via the private sector every year to budded stone fruits (peaches, nectarines, plums, and Almonds) on its, and that is cost many million dollars annually (Eliwa et al 2018). The use of conventional propagation methods in peaches is quite difficult because it results low multiplication rate (Stylianides et al 1989). Prunus rootstocks are difficult to propagate on a large scale with greenwood, soft, or hardwood cuttings due to poor rooting capacity (Eliwa 1994 Micropropagation offers a suitable method to provide the growers with sufficient quantities of rootstocks, as well as pathogen-free planting material for old and new cultivars (Balla and Mansvelt 2013).…”

Section: Introductionmentioning

confidence: 99%

In Vitro Explant Sterilization and Bud Proliferation Studies on Some Imported Peach Rootstocks

Eliwa,

El-Dengawy,

Gawish

et al. 2023

Preprint

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The present investigation was conducted in the Laboratory of Tissue Culture, Horticulture Department, Faculty of Agriculture, Damietta University, Egypt. The objective of this study was to establish a micropropagation protocol suitable for three imported peach rootstocks {Okinawa (P. persica), Nemared (P. persica × P. davidiana) × P. persica) and Garnem (P. dulcis × P. persica)} In vitro. According to the results, soaking the explants in sodium hypochlorite (NaOCl) at 20% for 15 min achieved the highest value of responsive (82.81%) and Survived (96.61%) with a minimum value of dead (3.14%) and contaminated (0.24%). Garnem genotype explants had achieved the best response (89.12%) and Survived (90.62%) with minimum dead (0.00%) and maximum contamination (9.37%). The shoot tips showed the highest responsive, Survived, and dead% (100, 87.40 and12.59% respectively) and minimum significant contaminated (0.00%). Also, the explants differed significantly in their percentages of responsive, survived, dead, and contaminated at the different collected dates. The 6-benzylaminopurine (BAP) concentrations used (3 to 5.0 mg/L-1) demonstrated similar behavior in terms of in vitro proliferation, with rates of 3.77 to 6.11, 4.33 to 8.88 and 3.33 to 7.44 shoots per explant for Okinawa, Nemared, and Garnem peach rootstocks respectively, indicating that the number of shoots proliferation is genotype dependent. Moreover, using 5.0 mg/L-1 BAP in combination with 0.2 mg/L-1 IBA produced higher significant values of average shoot proliferation % (96.29%) as well as number of shoots per explant (7.48) and average Leaves number/explant (16.33) than the other treatments. From these results, we could conclude that indole-butyric acid (IBA) synergists with 6-benzylaminopurine (BAP) improved adventitious bud development in vitro multiplication of Okinawa, Nemared, and Garnem peach rootstocks.

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“…However, they are difficult to be propagated on large scale through cuttings due to poor rooting capacity (Ammer, 1999;Alsalihy et al, 2004). The conventional propagation methods are quite difficult in peach (Stylianides et al, 1989). Moreover, the sexual propagation is not preferred due to the occurrence of undesired segregation (Davies and Duray, 2011).…”

Section: Introductionmentioning

confidence: 99%

Optimized Protocol for Micropropagation of Cadaman and Garnem Peach Rootstocks إستحداث بروتوکول للإکثار الدقيق لأصلي الخوخ الکادامان و الجارنم

Elyazid

Gawish

Eliwa

2021

Journal of Plant Production

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The present study aimed to develop a micropropagation protocol for Cadaman (P. persica x P. davidiana) and Garnem (P. persica x P. dulcis) peach rootstocks. Stem nodal segments with one axillary bud were used as explants. MS medium supplemented with different concentrations of BA with or without IBA was studied to determine the best for multiplication stage. The results revealed that MS medium supplemented with 2.0 mg/l BA+0.1 mg/l IBA was optimal for shoot proliferation of Cadaman rootstock, whereas MS containing 2.0 mg/l BA+ 0.0 IBA was advisable to Garnem peach rootstock. Concerning the effect of auxin type and concentration on the rooting stage, IBA at 1.0 mg/l was more effective on rooting induction of Cadaman shoots, whereas in case of Garnem rootstock both IBA and NAA auxins had a similar effect on the rooting process at the concentration of 1.0 mg/l individually. After acclimatization, survival rate was 83% and 63.3% for Cadaman and Garmen rootstocks, respectively.

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Resistance to Iron Deficiency of Five Peach Rootstocks

Cited by 9 publications

References 0 publications

Comprehensive Study on In vitro Propagation of Some Imported Peach Rootstocksa. In vitro Explant Surface Sterilization and Bud Proliferation

Comprehensive Study on In vitro Propagation of Some Imported Peach Rootstocksa. In vitro Explant Surface Sterilization and Bud Proliferation

In Vitro Explant Sterilization and Bud Proliferation Studies on Some Imported Peach Rootstocks

Optimized Protocol for Micropropagation of Cadaman and Garnem Peach Rootstocks إستحداث بروتوکول للإکثار الدقيق لأصلي الخوخ الکادامان و الجارنم

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