Resistance to Fusarium Wilt Race 2 in the Pisum Core Collection

McPhee, Kevin; Tullu, A.; Kraft, J. M.; Muehlbauer, F. J.

doi:10.21273/jashs.124.1.28

Cited by 39 publications

(21 citation statements)

References 10 publications

(7 reference statements)

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“…Altogether, this would indicate that the optimal method for resistance screening of M. truncatula collections to Fome is the traditional root-dipping method. This method was specifically developed for resistance screening or for assessing the efficiency of pathogen-control methods (Haglund 1989;De Cal et al 1999;McPhee et al 1999;Wang et al 1999;Chikh-Rouhou et al 2010;Bani et al 2012;Rispail and Rubiales 2014). This method, which involved uprooting seedlings from their substrate and dipping the root system in a solution of F. oxysporum spore before transplanting to new pots (Di Pietro and Roncero 1998), generally allowed a rapid and strong infection, as shown here for M. truncatula.…”

Section: Discussionmentioning

confidence: 99%

Resistance reaction of Medicago truncatula genotypes to Fusarium oxysporum: effect of plant age, substrate and inoculation method

2015

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Fusarium wilt, caused by several formae speciales of Fusarium oxysporum, is an important disease of most crop and pasture legumes, including field pea (Pisum sativum), chickpea (Cicer arietinum), lucerne (alfalfa, Medicago sativa) and barrel medic (M. truncatula). Medicago truncatula is an important pasture legume and a model legume species. Hence, it can be used to increase our knowledge of resistance mechanisms efficient to block F. oxysporum infection if its response to the disease is characterised. We evaluated the physiological and susceptibility responses to the disease of two contrasting M. truncatula genotypes, and the effect of several cultural conditions known to affect the disease incidence, such as plant age at infection time, growth substrate and the method of inoculation. Our results indicated that the A17 accession harbours a moderate level of resistance to the disease. We also showed that the method of inoculation strongly affected development of fusarium wilt disease in this model species, whereas it was not significantly altered by plant age or the inorganic growth substrate tested. In addition, we describe a rapid change in leaf temperature after infection, which can be used as an indirect parameter to confirm fungal infection at a very early stage of the interaction.

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Section: Discussionmentioning

confidence: 99%

Resistance reaction of Medicago truncatula genotypes to Fusarium oxysporum: effect of plant age, substrate and inoculation method

2015

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“…Race-specific resistance to F. oxysporum f.sp. pisi is controlled by single dominant genes for some of which markers are available (McPhee et al, 1999;Grajal-Martin and Muehlbauer, 2002;McClendon et al, 2002;Okubara et al, 2002). Resistance to verticillium wilt is quantitative (Pennypacker, 2000;Vandemark et al, 2006;Ben et al, 2013b).…”

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confidence: 99%

Achievements and Challenges in Legume Breeding for Pest and Disease Resistance

Rubiales

Fondevilla

Cao

et al. 2014

Critical Reviews in Plant Sciences

176

132

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“…Fusarium wilt race 1 was first encountered in Wisconsin in 1924 and later in other pea growing regions (Jones and Linford, 1925). Race 1 is now known to occur in pea production regions throughout the world (Haglund and Pepin, 1987;McPhee et al, 1999).…”

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DNA Markers Linked to Fusarium Wilt Race 1 Resistance in Pea

McClendon¹,

Inglis²,

McPhee³

et al. 2002

jashs

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Dry pea (Pisum sativum L.) production in many areas of the world may be severely diminished by soil inhabiting pathogens such as Fusarium oxysporum f. sp. pisi race 1, the causal organism of fusarium wilt race 1. Our objective was to identify closely linked marker(s) to the fusarium wilt race 1 resistance gene (Fw) that could be used for marker assisted selection in applied pea breeding programs. Eighty recombinant inbred lines (RILs) from the cross of Green Arrow (resistant) and PI 179449 (susceptible) were developed through single-seed descent, and screened for disease reaction in race 1 infested field soil and the greenhouse using single-isolate inoculum. The RILs segregated 38 resistant and 42 susceptible fitting the expected 1:1 segregation ratio for a single dominant gene (χ2 = 0.200). Bulk segregant analysis (BSA) was used to screen 64 amplified fragment length polymorphism (AFLP) primer pairs and previously mapped random amplified polymorphic DNA (RAPD) primers to identify candidate markers. Eight AFLP primer pairs and 15 RAPD primers were used to screen the RIL mapping population and generate a linkage map. One AFLP marker, ACG:CAT_222, was within 1.4 cM of the Fw gene. Two other markers, AFLP marker ACC:CTG_159 at 2.6 cM linked to the susceptible allele, and RAPD marker Y15_1050 at 4.6 cM linked to the resistant allele, were also identified. The probability of correctly identifying resistant lines to fusarium wilt race 1, with DNA marker ACG:CAT_222, is 96% percent. These markers will be useful for marker assisted breeding in applied pea breeding programs.

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Resistance to Fusarium Wilt Race 2 in the Pisum Core Collection

Cited by 39 publications

References 10 publications

Resistance reaction of Medicago truncatula genotypes to Fusarium oxysporum: effect of plant age, substrate and inoculation method

Resistance reaction of Medicago truncatula genotypes to Fusarium oxysporum: effect of plant age, substrate and inoculation method

Achievements and Challenges in Legume Breeding for Pest and Disease Resistance

DNA Markers Linked to Fusarium Wilt Race 1 Resistance in Pea

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