Resistance to fusarium basal rot of onion in greenhouse and field and associated expression of antifungal compounds

Özer, Nuray; Köycü, Nagehan Desen; Chilosi, G.; Magro, P.

doi:10.1007/bf02979850

Cited by 46 publications

(45 citation statements)

References 13 publications

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“…Our results showed a significant variation in virulence among F. oxysporum isolates from wilted Welsh onion from six different regions of Japan. These results agree with previous studies (Ö zer et al 2004;Galván et al 2008), which showed variation in virulence of F. oxysporum isolates on bulb onion.…”

Section: Discussionsupporting

confidence: 95%

Pathogenic variation and molecular characterization of Fusarium species isolated from wilted Welsh onion in Japan

et al. 2008

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Thirty-two isolates of Fusarium species were obtained from wilted Welsh onion (Allium fistulosum) grown on nine farms from six regions in Japan and identified as F. oxysporum (18 isolates), F. verticillioides (7 isolates), and F. solani (7 isolates). The pathogenicity of 32 isolates was tested on five commercial cultivars of Welsh onion and two cultivars of bulb onion in a seedling assay in a greenhouse. The Fusarium isolates varied in the degree of disease severity on the cultivars. Five F. oxysporum isolates (08, 15, 17, 22, and 30) had a higher virulence on the cultivars than the other isolates. The host range of these five isolates was limited to Allium species. Molecular characterization of Fusarium isolates was performed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of the internal transcribed spacer (ITS) regions of ribosomal DNA. The 32 isolates were grouped into eight types (four types for F. oxysporum, one for F. verticillioides, and three for F. solani). Restriction patterns of the ITS region were not related to pathogenicity. However, the haplotypes obtained with five enzymes (RsaI, HinfI, HaeIII, ScrFI, and MspI) and the phylogenetic analysis permitted the discernment of the three Fusarium species. The PCR-RFLP analysis should provide a rapid, simple method for differentiating Fusaruim species isolated from wilted Welsh onion in Japan.

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Section: Discussionsupporting

confidence: 95%

Pathogenic variation and molecular characterization of Fusarium species isolated from wilted Welsh onion in Japan

et al. 2008

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“…Based on the MDSI, the virulence of each isolate was recorded as low (MDSI: \2), moderate (MDSI: 2-3) or high (MDSI: [3) (Table 1). These results agree with those of previous studies, which showed variation in the virulence of F. oxysporum isolates from bulb onion (Ö zer et al 2004;Galván et al 2008) and Welsh onion (Dissanayake et al 2009). …”

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confidence: 96%

Genetic diversity and pathogenicity of Fusarium oxysporum isolated from wilted Welsh onion in Japan

et al. 2009

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Thirty isolates of Fusarium oxysporum from wilted Welsh onion plants were examined for their diversity in nucleotide sequences of the ribosomal DNA (rDNA) intergenic spacer (IGS) region and for pathogenicity with regard to five Welsh onion cultivars. Phylogenetic analysis based on the IGS sequences revealed polyphyletic origins of the isolates and a relationship between phylogeny and pathogenicity; low virulence isolates differed genetically from those with high and moderate virulence. Mating type analysis revealed that all F. oxysporum isolates were MAT1-1 idiomorphs, suggesting that the pathogens may be clonal in the fields examined.

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“…Variation in aggressiveness between F. oxysporum isolates pathogenic on onion was previously reported (Villeveille 1996;Özer et al 2004;Valdez et al 2004). Variation among Fusarium isolates might explain differences in response of resistant selections, as was suggested by C. Galmarini and J.…”

Section: Introductionmentioning

confidence: 55%

Genetic variation among Fusarium isolates from onion, and resistance to Fusarium basal rot in related Allium species

et al. 2008

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The aim of this research was to study levels of resistance to Fusarium basal rot in onion cultivars and related Allium species, by using genetically different Fusarium isolates. In order to select genetically different isolates for disease testing, a collection of 61 Fusarium isolates, 43 of them from onion (Allium cepa), was analysed using amplified fragment length polymorphism (AFLP) markers. Onion isolates were collected in The Netherlands (15 isolates) and Uruguay (9 isolates), and received from other countries and fungal collections (19 isolates).

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Resistance to fusarium basal rot of onion in greenhouse and field and associated expression of antifungal compounds

Cited by 46 publications

References 13 publications

Pathogenic variation and molecular characterization of Fusarium species isolated from wilted Welsh onion in Japan

Pathogenic variation and molecular characterization of Fusarium species isolated from wilted Welsh onion in Japan

Genetic diversity and pathogenicity of Fusarium oxysporum isolated from wilted Welsh onion in Japan

Genetic variation among Fusarium isolates from onion, and resistance to Fusarium basal rot in related Allium species

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